Aim of the study: Multi-drug resistant tuberculosis (MDR-TB) is still an
important public problem. Rapid diagnosis of the agent and determination
of resistance status are critical in establishing the correct treatment
protocol. This study was conducted with the aim of determining
resistance mutations against first and second line drugs in MDR-MTB
strain isolated from respiratory tract specimens . Material and method:
After the subculture of these strains in Löwenstein - Jensen medium, DNA
isolation was performed according to the boiling method. DNA isolates
were kept at -40 0C until the working day. Primer sequences specific to
rpoB, InhA, katG, gyrA, eis and rrs regions were used to determine
isoniazid, rifampicin, quinolone and aminoglycoside resistance. Results:
The positivity rate of rpoB, InhA, katG, gyrA, eis and rrs in 33 MDR-TB
isolates was 27 (81.8 %), 31 (93.9 %), 25 (75.7 %), 25 (75.7 %), 20
(60.6 %) and 14 (60.6 %), respectively. Resistance mutations were not
detected in susceptible isolates. Conclusion: According to the data
obtained from the study, it was found that fluoroquinolone resistance
mutations were higher in isolates defined as MDR-TB by conventional and
molecular methods, and in-house PCR method was a useful method for rapid
resistance detection.