Investigation of Contagious Agalactia by Bacteriological and PCR Methods in Sheep and Goats

Abstract

Investigation of Contagious Agalactia by Bacteriological and PCR Methods in Sheep and Goats AbstractThe aim of this study was diagnosis that occurrence of Contagious Agalactia by bacteriological and molecular methods in sheep and goats. A total of 339 samples from sheep and goats in Bursa, Balıkesir, Çanakkale and Edirne provinces were examined by bacteriological and molecular methods. The samples were 162 milk samples,147 eye swabs, 15 joint fluids, 11 nasal swabs and 4 lung tissue. In bacteriological examination, 29 isolates were evaluated as Mycoplasma sp.. As a result of biochemical tests and growth inhibition tests, 29 (8.55%) Mycoplasma sp. were identified as 25 (7.37%) Mycoplasma agalactiae, 2 (0.58%) Mycoplasma ovipneumoniae and 2 (0.58%) Mycoplasma arginini. In molecular diagnosis, polC gene-PCR results could be detected M. agalactiae positive with 9.14% rate. As a result of this, 5 milk samples and 1 lung tissue sample were detected positive by polC-PCR while negative by bacteriological examination. The results of polC-PCR detected M. agalactiae positive with 14.19% rate of milk samples, 13.33% rate of joint fluids, 2.72% rate of eye swabs and 50% rate of lung tissue samples but nasal swabs were detected as negative. In this study, presence of Contagious Agalactia were investigated by bacteriological and molecular methods and M. agalactiae was detected as a main agent which cause disease however other Mycoplasma species which cause disease were not observed.

Teke ve Koçların Prepusyal Svap Örneklerinden Genital Mikoplazma Türlerinin Karakterizasyonu

“…Rosendal [12] described it as occurring naturally in the genital tract of small ruminants. In recent years, M. arginini has been associated with various clinical signs in sheep and goats including pneumonia [13] . It is often thought to be a commensal or opportunistic organism and it is unusual for a mycoplasma species, in that it has been reported as occurring in many hosts, often with severe outcomes.…”

“…The use of PCR directly on the clinical sample or from washing of the clinical sample would allow for detection of both viable and non-viable Mycoplasma species. Use of culture enrichment also has the potential to further enhance the sensitivity of the PCR-DGGE method [13] .…”

The Investigation of the Infectious Agalactiae Infection in Sheep and Goat Milk Samples

“…(Table 5). In a study by Göçmen et al (2015) for investigating the infectious agalactiae disease in sheep and goats with bacteriological and PCR methods, Mycplasma sp. were identified in 29 samples of from 339 samples (162 milk samples, 147 eye swabs, 15 joint fluids, 11 nasal swabs and 4 lung tissues) collected from sheep and goats belonging to Canakkale and Edirne province.…”