Teke ve Koçların Prepusyal Svap Örneklerinden Genital Mikoplazma Türlerinin Karakterizasyonu

Abstract

Mycoplasma species are a major cause of mastitis, arthritis, pneumonia and reproductive disorders in small ruminants. Mycoplasmas in reproductive systems of males have been associated with diseases as orchitis, balanoposthitis and abnormal spermatozoa activity. The aim of this study was to detect Mycoplasma species that cause reproductive infections in bucks and rams. Total of 27 preputial swabs was collected from Saanen bucks and Kıvırcık rams at the Artificial Insemination (AI) Center of Uludag University in Turkey. Bacteriological culture methods, followed by Polymerase Chain Reaction (PCR) and Denaturing Gradient Gel Electrophoresis (DGGE) were used to detect and identify Mycoplasma species. The PCR-DGGE method identified one M. bovigenitalium and one M. arginini from two cases of orchitis in rams, and another M. bovigenitalium was identified from a buck with no clinical signs. The results showed that Mycoplasma species were present in the testicles of rams and bucks that were negative for Brucellosis, and likely causative organisms of orchitis which lead to reduced fertility.

Lesions of the prepuce and penis in rams: A retrospective study

“…During ejaculation, the urethral process rotates spirally, making a wave-like movement, and sprays the semen around the external cervical os of the female genital tract (Hafez 2000).In the literature, little can be found on the current incidence of prepuce and penis abnormalities in rams. The bestknown and studied are lesions caused by infectious agents such Orf virus (Gouletsou and Fthenakis 2015), ovine herpesvirus 2 (Pritchard et al 2008), enzootic posthitis (pizzle-rot) or ulcerative balanitis associated with Mycoplasma mycoides (Kidanemariam et al 2005;Gocmen et al 2019). The present retrospective study reports the incidence of pathological conditions of the penis and prepuce in Sarda rams either undergoing breeding soundness evaluation or admitted for genital disorders to the Section of Obstetrics and Gynaecology of the…”