Investigation of Antioxidant Properties of Polygonatum orientale Desf and Tilia dasystyla Extracts by Different Methods and Solvents

Abstract

Background: Free radicals cause many diseases in humans. Antioxidants reduce the risk of cardiovascular disease and stroke by neutralizing free radicals and on the other hand, prevent the progression of cancer. The natural antioxidant enhances the antioxidant properties of plasma to prevent diseases such as heart disease, cancer, and stroke. Secondary metabolites derived from plants have a strong potential for antioxidant activity. Methods: The purpose of this study was to investigate the antioxidant activities of two native plants species, including Polygonatum orientale Desf and Tilia dasystyla extracts, which are obtained with three different solvents (water, ethanol, and methanol) and two extraction methods (sonication and water bath) by DPPH (2,2-Diphenyl-1-Picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Potential) methods. This study is the first research to investigate the antioxidant properties of Polygonatum orientale Desf and Tilia dasystyla. Results: The results showed that methanol solvent had a higher efficiency among the other solvents in extracting of antioxidant compounds. Also, antioxidant level of Tilia dasystyla was higher than Polygonatum oriental Desf. In this regard, the highest FRAP values of Tilia dasystyla belonged to methanol solvent and water bath method with the amount of 2.72 ± 0.36 (mM). Whereas the highest FRAP values of Polygonatum orientae Desf belonged to methanol solvent and sonication method with the amount of 0.147 ± 0.01 (mM). Conclusions: Both plants showed antioxidant properties but Tilia dasystyla had higher antioxidant activities than Polygonatum orientale Desf. Methanol was more effective solvent in extracting antioxidant compounds and also water bath method was generally an effective method for extraction.

The effect of silver nanoparticles of aqueous Matricaria chamomilla extract on acute liver toxicity caused by acetaminophen in mice

“…Basil's strong action against free radicals can be attributed to the presence of these two substances. Basically, with the increase of total phenolic compounds, their antioxidant property also increases [23][24][25]. Hashemi et al [26] reported that the maximum amount of antioxidant activity in basil plant is under the effect of extract extraction by ultrasonic method and temperature of 45 degrees Celsius.…”

Total Phenolic and Flavonoid Content and Antibacterial Properties ofPolygonatum orientaleDesf andTiliadasystyla

“…According to Hanachi et al (2018), first methanol extracts showed more antioxidant properties than water extracts of T. dasytyla and P. orientale Desf (25). Thus, there is a direct correlation between phenolic compounds and antioxidant properties in these two plants.…”

“…Thus, there is a direct correlation between phenolic compounds and antioxidant properties in these two plants. Methanol solvent can extract more phenolic compounds and show more antioxidant properties in both plant species (25).…”

Antioxidant Activity and Phenolic Compounds Profile of Pistachio Skins (Pistacia vera L., cultivars Kallehghuchi and Ohadi)

“…Absolute methanol as blank and a sample containing absolute methanol 2mL and 1ml DPPH was considered as control. The absorption of the samples was read in a 517 nm (UNICO spectrophotometer UV/Vis 2100), and the percentage of free radicals cleared (RSA%) was calculated (14,15).…”