Investigation of Antioxidant and Enzyme Inhibition Activities and Phenolic Compound of Medicago rigidula (L.) All.

Çakmak, Yavuz Selim; Zengin, Gökhan; Eskin, Bülent; Yıldırım, Kevser; Topal, Müge; Aydın, Gözde Hatun; Ünlü, Emre; M, Baydemir; Erten, Kübra

doi:10.12991/marupj.311816

Cited by 5 publications

(2 citation statements)

References 12 publications

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“…Vol.65: e22210651, 2022 www.scielo.br/babt the pyocyanin pigment, which was shown to have antioxidant activity, can be used as a protective agent against neural injury and as an anticancer drug through its inhibitory activity on in vitro and cellular AChE enzyme activity. Studies in the literature have established the association between AChE-i activity and antioxidant activity [78][79][80][81]. Mounting evidence suggesting that neuroprotection and anticancer activity can be achieved by inhibiting AChE enzyme activity has transformed AChE enzyme activity into a guide for researchers who aim to develop novel effective agents against neurodegenerative diseases and cancer [82].…”

Section: Discussionmentioning

confidence: 99%

Pyocyanin Isolated from Pseudomonas aeruginosa: Characterization, Biological Activity and Its Role in Cancer and Neurodegenerative Diseases

Koyun

Şirin

Erdem

et al. 2022

Braz. arch. biol. technol.

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This study aimed to find a pigment of bacterial origin with protective/preventive activity against neurological injury and carcinogenesis, which may be used as a natural food additive in food activity or an active drug ingredient of an anti-neural injury/anticancer agent in the pharmaceutical industry. Within this scope, the Pseudomonas aeruginosa MB713 strain was used to produce pyocyanin. Characterization of the pyocyanin was carried out using UV-VIS, FT-IR, and GC-MS analyses. In addition, its inhibitory action on AChE and antioxidant activities were determined. The cytotoxic activities of the pyocyanin as well as its antiproliferative activities were detected. It was determined that the maximum production of the pyocyanin (51 μg/mL). The pyocyanin did not cause a cell death rate above 50% in L929 cells. The pyocyanin was found to exert an inhibitory effect on the AChE. The pyocyanin was found effective on all antioxidant parameters tested. The IC50 values of the pyocyanin on SK-MEL-30 and HT-29 cells were calculated as 72 and 179 μM, respectively. And due to the pyocyanin's antioxidant and inhibitory effects on AChE, antiproliferative effects, and protective effects against neurodegenerative injury, the use of the pyocyanin as HIGHLIGHTS• The maximum production of the pyocyanin was found to occur in MFDM and with shaking.• UV-VIS spectroscopy, GC-MS, and FT-IR analyses showed the presence of pyocyanin.• Pyocyanin had no cytotoxic impact on L929 cells (except 50-100 μM concentrations).• Pyocyanin had an antiproliferative impact on SK-MEL-30 cells.• Pyocyanin had in vitro and cellular AChE inhibitory and antioxidant properties.• The use of the pyocyanin as a neuroprotective agent or an anticancer agent is advised.

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Section: Discussionmentioning

confidence: 99%

Pyocyanin Isolated from Pseudomonas aeruginosa: Characterization, Biological Activity and Its Role in Cancer and Neurodegenerative Diseases

Koyun

Şirin

Erdem

et al. 2022

Braz. arch. biol. technol.

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“…Phosphate buffer and tyrosinase enzyme solution were added on it and incubated. After L-DOPA was added and incubated again for 10 minutes, absorbances were read at 492 nm (Orhan et al, 2012;Çakmak et al, 2017). Kojic acid was used as the standard substance.…”

Section: Enzyme Inhibition Analysismentioning

confidence: 99%

Thermopsis turcica Kit Tan, Vural&Küçüködük’ ün α-amilaz, α-glukozidaz, tirozinaz, asetilkolin esteraz enzim inhibisyon özellikleri ve uçucu yağ bileşimi

Aksoy¹,

Suyundikov²,

Düz³

2021

Anadolu Journal of Agricultural Sciences

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Thermopsis turcica Kit Tan, Vural & Küçüködük is a member of the legume family (Fabaceae), which has many plant species with economic value. It is one of the most narrowly distributed endemic plant species in Turkey. The most important feature that distinguishes Thermopsis turcica from other legume species is that it forms 3 fruits in one flower. α-amylase, α-glucosidase, acetylcholine esterase and tyrosinase inhibitory properties of different extracts and essential oil content of Thermopsis turcica were determined. Inhibition activities of enzymes were determined α-amylase using Caraway-Somoygi iodide/potassium iodide method, α-glucosidase with Palanisamy method, acetylcholine esterase enzyme with Ellman method and tyrosinase using dopachrome method. Hydrodestilation process (HD) was applied by using Clevenger device to determine the amount and components of essential oils and analyzed in GC-MS. The highest content of plant fatty acid was palmitic acid (51.8 %), myristic acid (17.5 %) and hexahydrofarnesyl acetone (5.3 %). It was determined that the plant did not show inhibitory activity against acetyl choline esterase and tyrosinase enzymes. Acetone and diethyl ether extracts have been shown to inhibit α-amylase. In particular, the diethyl ether extract has been found to exhibit an inhibitory activity close to the standard substance acarbose. Methanol and acetone extracts of the plant have a high inhibitory effect on the α-glucosidase enzyme. It is important with studies on the antidiabetic effects of this valuable species, which is in danger of extinction.

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Comparative study of in vitro antioxidant, acetylcholinesterase and butyrylcholinesterase activity of alfalfa (Medicago sativa L.) collected during different growth stages

et al. 2018

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Medicago sp. are often consumed as vegetable, salad and herbal tea as a form of fresh leaves and herbs. It is also very important in animal feeding, because it contains high percentage of protein and some important phytochemicals. In addition, traditionally, the leaves or other parts of the plant were used for treatment and preventing of kidney disorders, osteoporosis, anemia, diabetes, ulcer, coronary diseases, some cancers, and menopausal symptoms. In previous reports, it has been demonstrated that this plant has scavenging activity of the free radicals, which are involve in the development of the hypertension, Ischemia, neurodegenerative and rheumatoid diseases. In this study, the antioxidant, enzyme inhibitory activities of M. sativa L. collected in two growing period and phenolic substances that may be responsible for these activities were investigated. In this way, the beneficial effects of the plant will be revealed and a scientific work will be provided for the evaluation of the pharmaceutical and food industry.

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Investigation of Antioxidant and Enzyme Inhibition Activities and Phenolic Compound of Medicago rigidula (L.) All.

Cited by 5 publications

References 12 publications

Pyocyanin Isolated from Pseudomonas aeruginosa: Characterization, Biological Activity and Its Role in Cancer and Neurodegenerative Diseases

Pyocyanin Isolated from Pseudomonas aeruginosa: Characterization, Biological Activity and Its Role in Cancer and Neurodegenerative Diseases

Thermopsis turcica Kit Tan, Vural&Küçüködük’ ün α-amilaz, α-glukozidaz, tirozinaz, asetilkolin esteraz enzim inhibisyon özellikleri ve uçucu yağ bileşimi

Comparative study of in vitro antioxidant, acetylcholinesterase and butyrylcholinesterase activity of alfalfa (Medicago sativa L.) collected during different growth stages

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