Thermopsis turcica Kit Tan, Vural&Küçüködük’ ün α-amilaz, α-glukozidaz, tirozinaz, asetilkolin esteraz enzim inhibisyon özellikleri ve uçucu yağ bileşimi

Abstract

Thermopsis turcica Kit Tan, Vural & Küçüködük is a member of the legume family (Fabaceae), which has many plant species with economic value. It is one of the most narrowly distributed endemic plant species in Turkey. The most important feature that distinguishes Thermopsis turcica from other legume species is that it forms 3 fruits in one flower. α-amylase, α-glucosidase, acetylcholine esterase and tyrosinase inhibitory properties of different extracts and essential oil content of Thermopsis turcica were determined. Inhibition activities of enzymes were determined α-amylase using Caraway-Somoygi iodide/potassium iodide method, α-glucosidase with Palanisamy method, acetylcholine esterase enzyme with Ellman method and tyrosinase using dopachrome method. Hydrodestilation process (HD) was applied by using Clevenger device to determine the amount and components of essential oils and analyzed in GC-MS. The highest content of plant fatty acid was palmitic acid (51.8 %), myristic acid (17.5 %) and hexahydrofarnesyl acetone (5.3 %). It was determined that the plant did not show inhibitory activity against acetyl choline esterase and tyrosinase enzymes. Acetone and diethyl ether extracts have been shown to inhibit α-amylase. In particular, the diethyl ether extract has been found to exhibit an inhibitory activity close to the standard substance acarbose. Methanol and acetone extracts of the plant have a high inhibitory effect on the α-glucosidase enzyme. It is important with studies on the antidiabetic effects of this valuable species, which is in danger of extinction.