Investigation of antifungal effect of aqueous extract of garlic on Candida albicans (INVITRO)

Abstract

Background and aim: Candidiasis is a common fungal disease in the mouth. An appropriate treatment for candidiasis is the use of antifungal drugs. Due to drug resistance and known side effects of drugs, the tendency to use medicinal herbs is increasing. Garlic is identified as a strong antibacterial. Therefore, in this study, the effect of aqueous garlic extract and nystatin on Candida albicans was investigated.Materials and Methods: An experimental study was carried out. For each control and case group, one standard strain and 10 clinical strains were studied. The standard strain of Candida albicans was obtained from the Iranian Research Institute of Industrial Research and clinical strains from different patients. Garlic was prepared from the north of the country and was extracted by soaking method. Control groups containing nystatin suspension of 10,000 units / ml were prepared from Sigma, and finally, the diameter of the inhibition zone and MIC by Micro Broth Dialution method were studied. The results were analyzed by T test.Result: MIC of nystatin and the MIC and MBC of garlic show that Nystatin MIC was 3.49 ± 4.96 μg / ml and micronutrient was 1.42 ± 2.22 mg / ml and nystatin variation coefficient was 28% and garlic 15% as well as MBC of garlic equal to 58 /. ± 59.1 mg / ml with a coefficient of variation of 36%. In the meantime, mic is for standard samples of 78. 0 mg / ml and nystatin 0.5 μg / ml. The amount of nongrowth halo in nystatin was 29.1 ± 1.72 and in the garlic group was 20.1 ± 1.5, which was 9.1% or 31% higher in the nystatin group and the T test showed that this difference was statistically significant. (p <0/000) and the inferiority halo for standard sample 22 and nystatin 32. Conclusion:The study showed that the aqueous extract of garlic was able to inhibit the growth of Candida albicans but its effect was less than nystatin. .

Garlic (Allium sativum L.) Bioactives and Its Role in Alleviating Oral Pathologies

“…Thus, GE could be regarded as a better alternative in treating denture stomatitis [ 68 ]. Likewise, in vitro study the antifungal effect of AGE on C. albicans was investigated and they found that it was able to inhibit the growth of C. albicans, but its effect was less than nystatin [ 69 ].…”

Antimicrobial Activity of Aqueous Garlic Extract (Allium sativum) Against Porphyromonas gingivalis: An In-Vitro Study

“…(21) An investigation has shown the effect of aqueous garlic extract on Candida albicans (C. albicans) in the mouth. (22) The results of the cited study showed that the aqueous extract of garlic was able to inhibit the growth of C. albicans but its effect was less than that of nystatin. (22) Further research using different extraction methods and concentrations is suggested…”

Development of topical patches releasing allicin using garlic extract

“…It has been known to have hair growth stimulating properties 9 . Garlic ( Allium sativum ) from family Liliaceae has a wide range of medicinal properties, 10 it has antibacterial, 11 antiviral, 12 antifungal, 13 and antiprotozoal properties 14 . The main pharmacological effect of garlic is referred to presence of organosulfur volatile compounds, especially allicin which is produced when the garlic bulb is crushed 15,16 .…”

“…In addition, allicin has an immunomodulatory effect by enhancing the activity of macrophage during infection 22 . Moreover, it has antifungal properties against Candida albicans and antiprotozoal properties against amoeba parasite 13 …”

“…22 Moreover, it has antifungal properties against Candida albicans and antiprotozoal properties against amoeba parasite. 13 Some studies suggest that hair growth effect is attributed to the antibacterial and sterilizing properties of allicin and induction of vasodilation. 23 The mechanism of garlic actual effect is not entirely clear, whether it stimulates the hair growth or through its effect on the immune system.…”