Investigation of an active lipid in aqueous extracts of rabbit brain, and of some further hydroxy‐acids

Ambache, N.; Reynolds, M.; Whiting, Judith

doi:10.1113/jphysiol.1963.sp007104

Cited by 22 publications

(13 citation statements)

References 14 publications

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“…The results are shown in Table 14. The activity in terms of (Ambache & Reynolds, 1960Kirschner & Vogt, 1961;Ambache et a!., 1963;Toh, 1963). One of these substances has been isolated from ox brain and identified as prostaglandin F2a by chromatographic and mass spectrographic analysis (Samuelsson, 1964).…”

Section: Parallel Biological Assaysmentioning

confidence: 99%

Identification of Prostaglandins in Central Nervous Tissues of the Cat and Chicken

Horton

Main

1967

British Journal of Pharmacology and Chemotherapy

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Section: Parallel Biological Assaysmentioning

confidence: 99%

Identification of Prostaglandins in Central Nervous Tissues of the Cat and Chicken

Horton

Main

1967

British Journal of Pharmacology and Chemotherapy

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“…Most of the activity went into the acid ether phase. The active principle appears therefore to be a lipid acid resembling the hydroxy acid in extracts of rabbit brain (Ambache & Reynolds, 1960;Ambache, Reynolds & Whiting, 1963), i.e. an acid related to irin and the prostaglandins, which in relatively low concentrations caused contraction of the rat fundus preparation.…”

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confidence: 99%

Appearance of 5‐hydroxytryptamine and an unidentified pharmacologically active lipid acid in effluent from perfused cerebral ventricles

Feldberg¹,

Myers²

1966

The Journal of Physiology

125

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SUMMARY1. In cats anaesthetized with intraperitoneal pentobarbitone sodium, three regions of the cerebral ventricles, the third ventricle, the inferior or the anterior horn, were perfused with artificial c.s.f. and the effluent was tested on the fundus strip of the rat's stomach.2. Effluent from all three regions contracted the fundus strip. The contractions were due to at least two substances as revealed by treatment of the strip with 2-bromolysergic acid diethylamide (BOL). The contractions that were sensitive to BOL are attributed to 5-hydroxytryptamine (5-HT) whereas the BOL resistant contractions appear to be due to an unknown hydroxy acid related to irin or the prostaglandins.3. The contractions produced by effluent collected from the third ventricle were due wholly or mainly to 5-HT, those from the inferior horn to the unknown hydroxy acid, and those from the anterior horn to both substances in varying proportions. In addition, some samples of effluent from the third ventricle seemed to contain catecholamines as well.4. The 5-HT in the effluent from the third ventricle is thought to be derived from the hypothalamus. The amounts assayed in 1 ml. effluentthe volume collected during 10 or 20 min perfusion-varied between 0 4 and 12 ng 5-HT. Output of 5-HT was initially high, then usually decreased but sometimes increased again during prolonged perfusion when temperature began to rise as anaesthesia lightened or when additional pentobarbitone sodium was given intravenously.5. When perfusion of the third ventricle was continued after death the 5-HT content in the effluent increased 3 to 24-fold during the first hour and then gradually declined. This post mortem rise in 5-HT output * Supported by U.S

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“…3 (Ambache & Reynolds, 1960, 1961Ambache, Reynolds & Whiting, 1963). Both in the original crude extracts and in their ether-purified residues, however, the activity obtained per mg of brain tissue is usually lower than that of iris extracts from the same species, often considerably so.…”

Section: Rabbit Cerebral Hemisphere Extractsmentioning

confidence: 99%

A Simple Chemical Procedure for Distinguishing E From F Prostaglandins, With Application to Tissue Extracts

Ambache

Brummer

1968

British Journal of Pharmacology and Chemotherapy

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This paper describes an easy and relatively rapid procedure for distinguishing the ketonic prostaglandins E (PGEs) from the non-ketonic prostaglandins F (PGFs) and other hydroxy-acid lipid spasmogens. The method depends on the ability of certain hydrazine derivatives to combine specifically with keto groups. The condensation reaction leads to the formation of hydrazones according to the equation: RR'CO + H2N.NHR". 'RR'C=N.NHR" + H2OTo obtain this ketonic condensation we have used Girard's reagent T (trimethylammonium-acetohydrazide chloride, H2N.NH.CO.CH2.N(CH3)3C1), which is one of a series of reagents developed by Girard & Sandulesco (1936); these reagents are soluble in water and insoluble in non-hydroxylated organic solvents. The hydrazones formed from ketones and reagent T are also more water and less ether-soluble than the original ketones. In preliminary trials with known compounds it was found that treatment of ketonic PGEs in aqueous solution with reagent T leads, as expected, to an apparent retention of the product in the water phase during subsequent partitions with diethyl ether at pH 3-4; this reduced extractability of PGEs will be referred to as an apparent inactivation. On the other hand, the non-ketonic PGFs were recovered fully from the ether phase, indicating absence of reaction with the reagent, as expected from the difference in its structure at C9, which is shown above.Reagent T is itself insoluble in ether and did not interfere with the assays of prostaglandins.The reduction in ether-extractability of PGEs by reagent T was applied for the detection of prostaglandin-like spasmogenic keto-hydroxy-acids previously found in this laboratory in

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Investigation of an active lipid in aqueous extracts of rabbit brain, and of some further hydroxy‐acids

Cited by 22 publications

References 14 publications

Identification of Prostaglandins in Central Nervous Tissues of the Cat and Chicken

Identification of Prostaglandins in Central Nervous Tissues of the Cat and Chicken

Appearance of 5‐hydroxytryptamine and an unidentified pharmacologically active lipid acid in effluent from perfused cerebral ventricles

A Simple Chemical Procedure for Distinguishing E From F Prostaglandins, With Application to Tissue Extracts

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