“…Although PGF2a (03 jig/g) has been definitely identified in bovine brain by mass spectrometry (Samuelsson 1964), the identification and estimation of prostaglandins and prostaglandin-like substances within the central nervous system of dog, cat, and chick brain (Horton & Main 1967a;Holmes & Horton 1968a), as well as superfusates of cerebral cortex , cerebellum (Coceani & Wolfe 1965) and cerebroventricular system of the cat (Feldberg & Myers 1966), is tentative, and has depended upon the use of bioassay and thin-layer chromatography procedures similar to those described here. The symbols represent the specific areas of the AI chromatogram.…”