Investigating the effect of multi‐coated hydrogel layer on characteristics of electrospun <scp>PCL</scp> membrane coated with gelatin/silver nanoparticles for wound dressing application

Nguyen, Tien Ngoc-Thuy; Bui-Thuan, Thien; Ho, Minh Hieu; Tran, Nam H.; Dang, Nhi Ngoc‐Thao; Minh, Thai; Nguyen, Hoai Thi-Thu; Phan, Thắng Bách; Tran, Quyen Ngoc; Vo, Toi Van; Nguyen, Hoa

doi:10.1002/jbm.a.37222

Cited by 8 publications

(6 citation statements)

References 50 publications

(48 reference statements)

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“…It could be seen here and from our previous study [28] that the supportive effect of gelatin coating layer on proliferation of cells was overshadowed by the effect of AgNPs. This explains why PCLGelAg membrane in general did not result in better recovery compared to the other two commercial products.…”

Section: Discussionsupporting

confidence: 60%

“…In previous studies, we have fabricated an innovative electrospun polycaprolactone (PCL) membrane coated with multi-layers of gelatin (Gel) carrying AgNPs by immersing technique [26][27][28]. The PCLGelAg membrane (at third coating time) was proven to have sufficient tensile strength, excellent absorptive capacity, in vitro biocompatibility and effective bactericidal property on both Gram-positive and Gram-negative bacteria.…”

Section: Introductionmentioning

confidence: 99%

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The Efficacy of Silver-Based Electrospun Antimicrobial Dressing in Accelerating the Regeneration of Partial Thickness Burn Wounds Using a Porcine Model

Thuan

Nguyen

et al. 2021

Polymers

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(1) Background: Wounds with damages to the subcutaneous are difficult to regenerate because of the tissue damages and complications such as bacterial infection. (2) Methods: In this study, we created burn wounds on pigs and investigated the efficacy of three biomaterials: polycaprolactone-gelatin-silver membrane (PCLGelAg) and two commercial burn dressings, Aquacel® Ag and UrgoTulTM silver sulfadiazine. In vitro long-term antibacterial property and in vivo wound healing performance were investigated. Agar diffusion assays were employed to evaluate bacterial inhibition at different time intervals. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and time-kill assays were used to compare antibacterial strength among samples. Second-degree burn wounds in the pig model were designed to evaluate the efficiency of all dressings in supporting the wound healing process. (3) Results: The results showed that PCLGelAg membrane was the most effective in killing both Gram-positive and Gram-negative bacteria bacteria with the lowest MBC value. All three dressings (PCLGelAg, Aquacel, and UrgoTul) exhibited bactericidal effect during the first 24 h, supported wound healing as well as prevented infection and inflammation. (4) Conclusions: The results suggest that the PCLGelAg membrane is a practical solution for the treatment of severe burn injury and other infection-related skin complications.

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Section: Discussionsupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

The Efficacy of Silver-Based Electrospun Antimicrobial Dressing in Accelerating the Regeneration of Partial Thickness Burn Wounds Using a Porcine Model

Thuan

Nguyen

et al. 2021

Polymers

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“…Moreover, after 90 days, the nanocomposite films were completely degraded, indicating that the AgNPs did not affect the degradability of WPU-g-GH. As the AgNP content increased, the biodegradation rate of the nanocomposite films increased, whereas the weight loss was extremely small, confirming chelation between AgNP/WPU-g-GH and its role in dispersing and adhering the AgNPs to the films . Further, the degradation process of the AgNP/WPU-g-GH nanocomposite films was controllable and relatively long, meeting the requirements of modern biomedical materials.…”

Section: Resultsmentioning

confidence: 68%

“…As the AgNP content increased, the biodegradation rate of the nanocomposite films increased, whereas the weight loss was extremely small, confirming chelation between AgNP/WPU-g-GH and its role in dispersing and adhering the AgNPs to the films. 55 Further, the degradation process of the AgNP/WPU-g-GH nanocomposite films was controllable and relatively long, meeting the requirements of modern biomedical materials. The results reveal that the prepared AgNP/WPU-g-GH show excellent biodegradability, which is essential for preparing GH-based biomass materials in the biomedical field.…”

Section: Resultsmentioning

confidence: 92%

Silver Nanoparticle-Loaded Gelatin-Based Nanocomposite Films toward Enhanced Mechanical Properties and Antibacterial Activity

Yang

Zhang

Wang

et al. 2022

ACS Appl. Bio Mater.

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Gelatin (GH) is a natural polymer material with unique physical, chemical, and biological properties that render it a good base material for biomedical material production. Herein, Ag nanoparticles (NPs) were loaded onto a waterborne polyurethane−GH composite (WPU-g-GH) to prepare a GH-based nanocomposite (AgNP/WPU-g-GH) films). The prepared nanocomposite films were characterized using several analyses including Fourier transform infrared spectroscopy, ultraviolet−visible spectroscopy, X-ray diffraction, transmission emission microscopy, mechanical strength tests, and other analyses. The results demonstrated that the nanocomposite films had high mechanical strength, good thermal stability, and controllable biodegradability. In particular, when the AgNP loading content was 0.03%, the tensile strength, elongation at break, and average particle size of the nanocomposite film reached 45.13 MPa, 476.04%, and 13.02978 ± 1.64406 nm, respectively. Disk diffusion and cytotoxicity analyses revealed that the nanocomposite films exhibited significant antibacterial activity against Gram-negative and Gram-positive bacteria without affecting the cell viability of fibroblasts. These findings indicate that the nanocomposite films with high mechanical strength and antibacterial activity could be used for wound management, tissue adhesion, and biomaterial surface coating.

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“…Recently, many studies demonstrated that metallic oxide-incorporated electrospun nanofiber membranes show great capacity in inhibiting bacterial infection and accelerating wound healing. [13][14][15][16][17] Despite promising outcomes, the underlying mechanisms are not fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of magnesium oxide‐incorporated electrospun membrane modulating inflammation and accelerating wound healing

Liu

Zhang

Chen

et al. 2022

J Biomedical Materials Res

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Previously, we demonstrated that magnesium oxide (MgO)‐incorporated electrospun membranes show powerful antibacterial activity and promote wound healing, but the underlying mechanisms have not been entirely understood. Herein, we investigated the relationship between structure and function of MgO‐incorporated membranes and interrogated critical bioactive cues that contribute to accelerated wound healing and functional restoration. Our results show that MgO‐incorporated membranes exhibit good flexibility and improved water vapor transmission rates (WVTRs) and sustained Mg2+ release in a simulated model of wounds. MgO‐incorporated membranes modulate macrophage phenotype to downregulate inflammatory response, contributing to alleviated inflammation and creating a favorable microenvironment for wound healing. Specifically, MgO‐incorporated membranes stimulate macrophages to shift to a pro‐healing M2 phenotype and upregulate pro‐healing cytokine of transforming growth factor‐beta 1 (TGF‐β1) and downregulate pro‐inflammatory cytokines under lipopolysaccharide (LPS) challenge conditions. Together with increased TGF‐β1 by macrophages, MgO‐incorporated membranes significantly boost the proliferation of fibroblasts and upregulate collagen production, thus driving granulation tissue formation and wound closure. MgO‐incorporated membranes promote angiogenesis by promoting tube formation and upregulating vascular endothelial growth factor (VEGF) production of endothelial cells. Rapid epithelialization of regenerated skin tissue is attributed to the balanced phenotype of keratinocytes between proliferative and terminally differentiated populations. In addition to coordinating keratinocyte phenotype, MgO‐incorporated membranes reduce the expression of inflammatory cytokine interleukin 1‐alpha (IL‐1α) therefore promoting hair follicle regeneration. These data provide mechanisms of MgO‐incorporated membranes that inhibit bacterial infection, alleviate inflammation, facilitate extracellular matrix production and epithelialization, and potentiate hair follicle regeneration.

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Investigating the effect of multi‐coated hydrogel layer on characteristics of electrospun PCL membrane coated with gelatin/silver nanoparticles for wound dressing application

Cited by 8 publications

References 50 publications

The Efficacy of Silver-Based Electrospun Antimicrobial Dressing in Accelerating the Regeneration of Partial Thickness Burn Wounds Using a Porcine Model

The Efficacy of Silver-Based Electrospun Antimicrobial Dressing in Accelerating the Regeneration of Partial Thickness Burn Wounds Using a Porcine Model

Silver Nanoparticle-Loaded Gelatin-Based Nanocomposite Films toward Enhanced Mechanical Properties and Antibacterial Activity

Mechanisms of magnesium oxide‐incorporated electrospun membrane modulating inflammation and accelerating wound healing

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