Investigating the binding interaction of azur A with hyaluronic acid via spectrophotometry and its analytical application

Chen, Q.; Li, X. L.; Liu, Q.; Jiao, Qingcai; Cao, Wen-gen; Wan, Hang

doi:10.1007/s00216-005-3337-7

Cited by 11 publications

(7 citation statements)

References 15 publications

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“…However, we were somewhat surprised that this relatively low-charged dye (when compared with MalB) could apparently still operate under such competitive conditions. Interestingly, the binding of AA to hyaluronic acid and heparin has been probed previously, with studies showing increasing electrolyte concentration disrupted the AA-substrate interaction. pH was also demonstrated to affect the response of AA regardless of which absorption maximum was monitored.…”

mentioning

confidence: 94%

Mallard Blue: A High-Affinity Selective Heparin Sensor That Operates in Highly Competitive Media

et al. 2013

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We report the simple synthesis and full investigation of a novel heparin binding dye, mallard blue, an arginine-functionalized thionine. This dye binds heparin in highly competitive media, including water with high levels of competitive electrolyte, buffered aqueous solution and human serum. The dye reports on heparin levels by a significant change in its UV–vis spectroscopic profile. Molecular dynamics modeling provides detailed insight into the binding mode. Heparin binding is shown to be selective over other glycosaminoglycans, such as hyaluronic acid and chondroitin sulfate. Importantly, we demonstrate that, in the most competitive conditions, mallard blue outperforms standard dyes used for heparin sensing such as azure A.

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confidence: 94%

Mallard Blue: A High-Affinity Selective Heparin Sensor That Operates in Highly Competitive Media

et al. 2013

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“…It is very likely that AA molecules that are close to a PS macromolecule will block other molecules that are far from the PS macromolecule from approaching the PS macromolecule at high AA concentrations. This self-interference of AA on AA -PS interaction, which was also reported for the AA binding interaction to hyaluronic acid, [12] suggests that the molecular weights of polysaccharides such as PS in this work can easily be overestimated if the N value obtained at high AA concentrations is used to calculate the molecular weight. …”

Section: Q Chen Et Al 92mentioning

confidence: 64%

“…The mechanism of positively charged dyes interacting with negatively charged glycosaminoglycans was proposed by Jiao et al [10,11] A mathematical model based on this mechanism agreed very well with experimental results. [12] Based on this mechanism, Chen et al [12] proposed an improved method of estimating the molecular weight of the sulfated polysaccharides. However, interaction of pachyman sulfate with azur A was not studied.…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic Characterization of Pachyman Sulfate and Its Binding Interaction with Azur A

Chen

Wang

et al. 2007

Spectroscopy Letters

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High-purity pachyman isolated from the sclerotium of Poria cocos was reacted with chlorosulfonic acid to obtain pachyman sulfate (PS). The FTIR spectrum indicated sulfation of glucoside hydroxyl. The NMR results indicated almost complete sulfation on C-6, C-2, and C-4 of glucoside. Interaction between azur A (AA) and PS with AA concentration from 2.745 Â 10 25 to 6.863 Â 10 25 M was investigated using spectrophotometry at 625 nm. It was found that AA was a promising spectroscopic probe for the detection of sulfated groups in the PS macromolecule. Self-interference of AA on AA -PS interaction caused an overestimation of the molar mass of PS. The molecular weight mass of 13,110 Da obtained with an improved method for PS used in the current work agreed well with the value of 10,233 Da calculated from HPLC results.

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“…Similar phenomenons were observed in the interactions between heparin and methylene blue, 22 azur A and hyaluronic acid. 23 The described AR-APS quenching is mainly due to static fluorescence quenching. Interaction of the fluorophore AR with the quencher APS forms a non-fluorescent complex (as shown in Figure 2).…”

Section: Intermolecular Weak Interactions Between Ar and Aps And Specmentioning

confidence: 99%

A Novel Fluorescence Quenching Method for the Determination of Aloe Polysaccharide

Gu¹,

Song²,

Wen³

et al. 2011

Chin. J. Chem.

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Alizarin red (AR) can bind with aloe polysaccharide (APS) in doubly de-ionized water to form a red complex resulting in fluorescence quenching of it. The maximum fluorescence quenching wavelength is 572 nm. The chromogenic reaction is rapid and the fluorescence intensity remains stable for at least 2 h at room temperature. for APS and the mean recovery (100.2±2)%, RSD≤3%. The effect of various substances on the determination of APS was also investigated in detail, and the results show that most of the studied coexistent substances can be tolerated in considerable amounts. The proposed method is sensitive, simple, fast and suitable for routine assay.

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Investigating the binding interaction of azur A with hyaluronic acid via spectrophotometry and its analytical application

Cited by 11 publications

References 15 publications

Mallard Blue: A High-Affinity Selective Heparin Sensor That Operates in Highly Competitive Media

Mallard Blue: A High-Affinity Selective Heparin Sensor That Operates in Highly Competitive Media

Spectroscopic Characterization of Pachyman Sulfate and Its Binding Interaction with Azur A

A Novel Fluorescence Quenching Method for the Determination of Aloe Polysaccharide

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