A Novel Fluorescence Quenching Method for the Determination of Aloe Polysaccharide

Abstract

Alizarin red (AR) can bind with aloe polysaccharide (APS) in doubly de-ionized water to form a red complex resulting in fluorescence quenching of it. The maximum fluorescence quenching wavelength is 572 nm. The chromogenic reaction is rapid and the fluorescence intensity remains stable for at least 2 h at room temperature. for APS and the mean recovery (100.2±2)%, RSD≤3%. The effect of various substances on the determination of APS was also investigated in detail, and the results show that most of the studied coexistent substances can be tolerated in considerable amounts. The proposed method is sensitive, simple, fast and suitable for routine assay.