Investigating Helicobacter pylori in nasal polyposis using polymerase chain reaction, urease test and culture

Nemati, Shadman; Mojtahedi, Ali; Naghavi, Seyyed Ebrahim; Banan, Rahmatollah; Zia, Firoozeh

doi:10.1007/s00405-011-1848-8

Cited by 20 publications

(18 citation statements)

References 12 publications

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“…It has to be freshly prepared before applying the test, making it time consuming. Szczygielski et al [36] and Nemati et al [33] could not prove any association between H. pylori and nasal polyps on the basis of urease test. Khademi et al [24] and Kaviani et al [19] reported false positivity in their study due to some other bacteria with urease activity.…”

Section: Resultsmentioning

confidence: 94%

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Detection of Helicobacter pylori in Nasal Polyps

Bansal

Sharma

Agarwal

et al. 2016

Head and Neck Pathol

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To detect the presence of Helicobacter pylori in nasal polyps. A case-control study was conducted enrolling 35 patients with nasal polyps (cases) and patients undergoing septoplasty (controls). Fresh tissue samples were used for urea broth test and imprint cytology, while formalin fixed tissue sections were used for morphology, special stains and immunohistochemistry for H. pylori. Fresh stool samples from both groups were tested to correlate the gastrointestinal status. H. pylori was detected in 40.0 % (14/35) of cases and 8.5 % of controls (3/35) (p = 0.004) by immunohistochemistry. Amongst cases, eight were positive with urea broth test, six with imprint cytology (Giemsa stain), three with H & E, and nine with modified McMullen's stain. Hyperplasia of the lining epithelium and lymphoid aggregates were significantly noticed in nasal polyps positive for H. pylori. Stool antigen test was positive in subjects who were positive for H. pylori in the nasal mucosa. There appears to be an association between H. pylori and nasal polyps. Immunohistochemistry is more sensitive and specific method to detect H. pylori.H. pylori induced inflammatory tissue reaction pattern indicates a possible causal association. Further studies are needed to prove the causal relationship between H. pylori and nasal polyps.

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Section: Resultsmentioning

confidence: 94%

“…Burduk et al [21] believed that PCR assay is an appropriate method for detection of H. pylori in biopsy specimens, as culture is difficult to achieve. However, Nemati et al [33], Abbasi et al [34], and Ozyurt et al [20] could not yield a significant result employing PCR.…”

Section: Resultsmentioning

confidence: 96%

Detection of Helicobacter pylori in Nasal Polyps

Bansal

Sharma

Agarwal

et al. 2016

Head and Neck Pathol

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“…Nemati (29) , who found no intranasal H. pylori in any subjects, had excluded GORD sufferers from the study.…”

Section: Pathogenic Factorsmentioning

confidence: 99%

Association of gastro-oesophageal reflux and chronic rhinosinusitis: systematic review and meta-analysis

Leason¹,

Barham²,

Oakley³

et al. 2017

Rhin

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Introduction: Gastro-oesophageal reflux disease (GORD) has been implicated in the development of chronic rhinosinusitis (CRS).The association of GORD with CRS is systematically assessed from the medical literature.Methodology: Embase and MEDLINE were searched using a comprehensive strategy limited to English language and Human subjects. Any study with original data on the experimental, diagnostic, treatment or prognostic association of CRS with GORD was included. Studies without a control group, case reports and review articles were excluded. Results:The search returned 958 records, with an additional 10 found from bibliographic lists; this produced 32 studies. The included studies (n=32) consisted of studies reporting pathogenic factors (n=20), epidemiological association (n=8), prognostic interactions (n=3), and a combination of these outcomes (n=1). Potential pathogenic roles for GORD in CRS were supported; CRS subjects had greater prevalence of intranasal Helicobacter pylori and acid reflux than subjects without CRS. CRS is more prevalent in GORD sufferers than those without GORD. Evidence is conflicting for GORD as a factor in CRS treatment failure. Conclusion:The results support a significant association of GORD with CRS. Physicians should be cognizant of the potential for acid and non-acid reflux as a driving factor in CRS.

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“…19,21 Nemati et al showed culture test negative in his studies, where as in our study 3 (8.57%) cases showed positive growth in culture media. 22 So IHC and culture test can be regarded as highly sensitive and specific which can detect the low concentration of bacteria and truly positive cases, so the true incidence of H. pylori in our study is 3 (8.57%). Culture is cheap and easily available method when compare to IHC which is costly and not easily available and particularly used in research purpose.…”

Section: Resultsmentioning

confidence: 71%

Association of Helicobacter pylori in nasal polyposis

Nandyal¹,

Naik²,

Sahana³

et al. 2018

Int J Otorhinolaryngol Head Neck Surg

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Background: A significant number of patients with gastro esophageal reflux disease (GERD) disease are actually infected with Helicobacter pylori, transmission of the bacteria from stomach to nose may occur. Few studies have revealed the presence of Helicobacter pylori in normal nasal mucosa, in patients with chronic rhino sinusitis and in nasal polyps but the results have been conflicting and further studies are required for its establishment. The aims and objectives of the study were to study the presence of Helicobacter Pylori in nasal polyp specimen by using non specific tests- rapid urease test, Giemsa stain and H&E stain, specific tests-immunohistochemistry and culture.Methods: 35 patients with nasal polyp were underwent endoscopic nasal polypectomy. Specimens were analyzed for presence of Helicobacter pylori by urease test, Giemsa stain, Hemotoxylin and Eosin stain, Immunohistochemistry and culture. Results: Out of 35 patients 10 patients had gastro esophageal reflux disease (GERD), in which 06 patients showed positive for urease test and Giemsa stain and 3 patients picked up by immunohistochemistry test and growth in culture media. Of which Helicobacter pylori positive cases by H&E stain showed typical hyperplastic epithelium and lymphoid aggregation.Conclusions: There exists an association between H. pylori and nasal polyps and GERD. Non-specific urease test and modified Giemsa stain were positive in considerable number of cases, but the statistical significance could not be established. Immunohistochemistry and culture can be regarded as the procedure of choice in truly positive cases infected with H. pylori along with hyperplastic epithelium in H & E stain, all with co-relative finding but require larger study to establish their association which will help in further research and treatment.

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Investigating Helicobacter pylori in nasal polyposis using polymerase chain reaction, urease test and culture

Cited by 20 publications

References 12 publications

Detection of Helicobacter pylori in Nasal Polyps

Detection of Helicobacter pylori in Nasal Polyps

Association of gastro-oesophageal reflux and chronic rhinosinusitis: systematic review and meta-analysis

Association of Helicobacter pylori in nasal polyposis

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