Inverse Association between miR-194 Expression and Tumor Invasion in Gastric Cancer

Song, Yongxi; Zhao, Feng; Wang, Zhenning; Liu, Zhuangkai; Chiang, Yeunpo; Xu, Yingying; Gao, Peng; Xu, Hui

doi:10.1245/s10434-011-1999-2

Cited by 58 publications

(52 citation statements)

References 33 publications

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“…In addition, in cancer of the gut (37,38) and kidney (39), miR-194 served a similar inhibitory role. By contrast, it was suggested that miR-194 served a role in the promotion and progression of breast cancer (9) and pancreatic ductal adenocarcinoma (40).…”

Section: Discussionmentioning

confidence: 94%

“…By contrast, it was suggested that miR-194 served a role in the promotion and progression of breast cancer (9) and pancreatic ductal adenocarcinoma (40). These studies indicated that miR-194 had various target genes (31)(32)(33)(34)(35)(36)(37)(38)(39)(40). Previous studies have demonstrated that miR-194 inhibited cell migration and invasion, which may have contributed to the anti-tumor activity of trastuzumab on HER2-overexpressing breast cancer cells (41,42).…”

Section: Discussionmentioning

confidence: 99%

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Promotional effect of microRNA-194 on breast cancer cells via targeting F-box/WD repeat-containing protein 7

et al. 2018

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Abstract. Breast cancer is the most common type of malignant cancer in females. An increasing number of studies have revealed that microRNAs (miR), which belong to a class of small non-coding RNAs, serve an important role in a number of human cancer subtypes. In the present study, the role of miR-194 in breast cancer cells and its underlying mechanisms were investigated. The results demonstrated that the serum levels of miR-194 were significantly higher in patients of the poorly differentiated and well-differentiated groups, compared with in healthy adults. Additionally, the serum level of miR-194 was significantly higher in the poorly differentiated group compared with in the well-differentiated group. In order to further investigate the role of miR-194 in breast cancer cells, the present study transfected two breast cancer cell lines, MCF-7 and MDA-MB-231, with an empty vector (control), miR-194 (overexpression), antagomiR-194 (inhibitor, functional knock down) or antagomiR-194 and miR-194. An MTT assay was performed in order to detect the proliferation of breast cancer cells in the various groups. The results revealed that the overexpression of miR-194 significantly accelerated cell proliferation, whereas the inhibition of miR-194 significantly decelerated the proliferation of MCF-7 and MDA-MB-231 cells. Furthermore, the expression levels of cyclin D and cyclin E were significantly upregulated in miR-194 overexpressing cells, and the expression levels of cyclin D and cyclin E were significantly downregulated in miR-194 inhibited cells, as compared with in control cells. No significant change was observed in the level of proliferation of cells co-transfected with miR-194 and antagomiR-194, compared with in the control cells. According to the hypothesis suggesting possible target genes of miR-194, the present study proposed that F-box/WD repeat-containing protein 7 (Fbxw-7) may be a direct target of miR-194, which was confirmed by a luciferase reporter assay. The present study suggested that miR-194 expression promoted the proliferation of breast cancer cells by targeting Fbxw-7, and may serve as a biomarker and a novel target for breast cancer therapy.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Promotional effect of microRNA-194 on breast cancer cells via targeting F-box/WD repeat-containing protein 7

et al. 2018

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“…In these studies, dramatic decreases in miR-194 expression were found in hepatocellular carcinoma (HCC; refs. 20, 21), CRC (22), gastric cancer (23), and multiple myeloma (24), suggesting its tumor-suppressive function. Chiang and colleagues also confirmed miR-194 as a tumor-suppressor gene in patients with CRC (22), whereas another study revealed a proangiogenesis function of miR-194 in HCT116 cells (25).…”

Section: Discussionmentioning

confidence: 99%

miR-194 as a Predictor for Adenoma Recurrence in Patients with Advanced Colorectal Adenoma after Polypectomy

Wang

Ren

Zheng

et al. 2014

Cancer Prevention Research

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microRNAs (miRNA) are promising predictors in colorectal cancer (CRC). We investigated whether miRNAs could predict adenoma recurrence in patients with advanced colorectal adenoma (ACRA) after polypectomy. miRNA expression profiling was performed by miRNA microarray to identify recurrencerelated miRNAs. Candidate miRNAs extracted from formalin-fixed paraffin-embedded blocks of patients with ACRA were measured using real-time PCR. Logistic regression analysis was conducted to investigate whether validated miRNA expression profiles were independent from other known adenoma recurrence risk factors. The prognostic values of six miRNAs and three independent risk factors were assessed by the area under the receiver operating characteristic (ROC) curve analysis. The expressions of six candidate miRNAs were significantly decreased from levels in normal colorectal tissue compared with ARCA with adenoma recurrence (RACRA) in this retrospective cohort. However, only miRNA (miR)-194 emerged as a practical predictor. The sensitivity and specificity of miR-194 as a predictor were 71.0% and 78.0%, respectively, at a cutoff value of 0.1311 in the retrospective cohort. Sensitivity and specificity were 76.1% and 77.2%, respectively, in the prospective cohort using the same cutoff value. Low expression levels of miR-194, adenoma size !2 cm, and !3 adenomas were independent risk factors for adenoma recurrence. Moreover, low expression of miR-194 was a better predictor of adenoma recurrence than the adenoma size and numbers according to ROC curve analysis. miR-194 may be an independent predictor for adenoma recurrence in patients with ACRA after polypectomy. Cancer Prev Res; 7(6); 607-16. Ó2014 AACR.

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“…The function of these seven microRNAs in metastasis is not clear. Among those five up-regulated microRNAs in SW620, miR-194 has been shown to be a suppressor of metastasis in endometrial cancer, liver cancer and gastric cancer [34][35][36]. MiR-375 can inhibit tumor growth and metastasis in oesophageal squamous cell carcinoma by repressing insulin-like growth factor 1 receptor [37] and inhibit metastasis in squamous cervical cancer by targeting Fig.…”

Section: Discussionmentioning

confidence: 99%

miR-200c inhibits invasion and migration in human colon cancer cells SW480/620 by targeting ZEB1

Chen¹,

Liang²,

Wang³

2012

Clin Exp Metastasis

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MicroRNAs are a class of ≈22-nt noncoding single-strand RNAs regulating gene expression postscriptionally. Metastasis caused poor prognosis in colorectal cancer patients and half of the patients developed metastatic lesions when admission. Here we investigated the possible roles of microRNAs in regulating metastasis in the paired colon cancer cells SW480 and SW620. Among those dysregulated microRNAs, miR-200c was speculated to inhibit metastasis by targeting ZEB1. Overexpression of miR-200c was concurrent with downregulation of ZEB1 mRNA and protein. Functional assays demonstrated that modulation of miR-200c with mimics or inhibitors changed potential of metastasis in SW480/620 cancer cells in vitro. Taken together, our study demonstrated that miR-200c inhibits metastatic ability by targeting ZEB1 in colon cancer cells SW480/620 and suggested that modulation of miR-200c could serve as therapeutic tool for inhibiting metastasis in colorectal cancer.

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Inverse Association between miR-194 Expression and Tumor Invasion in Gastric Cancer

Abstract: These findings imply that miR-194 might play an important role in gastric cancer invasion and progression.

Cited by 58 publications

References 33 publications

Promotional effect of microRNA-194 on breast cancer cells via targeting F-box/WD repeat-containing protein 7

Promotional effect of microRNA-194 on breast cancer cells via targeting F-box/WD repeat-containing protein 7

miR-194 as a Predictor for Adenoma Recurrence in Patients with Advanced Colorectal Adenoma after Polypectomy

miR-200c inhibits invasion and migration in human colon cancer cells SW480/620 by targeting ZEB1

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