“…A study to compare the conventional isolation methods vs molecular identification procedure to detect Salmonella in broiler chicken meat has revealed that prevalence of Salmonella in meat samples was 12% in molecular method whereas it was 22% in the conventional method (Ibrahim et al, 2014). Use of PCR by targeting the sequences of hns and invA genes for rapid detection of Salmonella has been proven earlier too (El-Sebay et al, 2017). As a unique sequence to this genus available in fragment of invA gene, it has been verified as a precise PCR target (Rahn et al, 1992).…”
Section: Isolation Identification Confirmation and Serotyping Of Samentioning
Purpose : Salmonella infections continue to be a global problem with millions of humans and animal cases occurring annually. Broiler chicken plays a significant role causing Salmonella infections in Sri Lanka. Consumption of food contaminated with antimicrobial resistant Salmonella aggravates the problem. This study isolated, identified, and serotype the Salmonella spp. from broiler chicken meat in Sri Lanka and examined their antimicrobial susceptibility to be used in establishment of control measures. Research Method : Isolation of Salmonella species from broiler chicken meat was done by conventional method of isolation followed by polymerase chain reaction (PCR) confirmation. All PCR confirmed isolates of Salmonella were serotype and then, isolates were tested for antibiotic susceptibility using disc diffusion assay followed by the detection of antibiotic resistance genes using PCR. Findings : Broiler chicken meat in Sri Lanka is contaminated with Salmonella spp. at the prevalence of 11.6% and 8.9% of them carried hns and invA specific genes. Isolates were serotyped as Salmonella Typhimurium (47.8%), Salmonella Enteritidis (26.1%) and non typable (26.1%). Three isolates were resistant to ampicillin. Intermediate resistance was shown to three antibiotics and all the isolates were sensitive to nine antibiotics. Majority (56.5%) of Salmonella were sensitive to all the tested antibiotics. Prevalence of resistant genes for tetracycline, sulfonamides and aminoglycosides were within 4%-26%. None of the isolates except one (4%) carried chloramphenicol resistance genes. Originality / Value : Steps must be taken to minimize contamination of broiler chicken meat with Salmonella spp in Sri Lanka. Although, there is a low prevalence of antibiotic resistant isolates, its mere presence in broiler chicken is a warning signal of possibility of emergence of multidrug resistant strains.
“…A study to compare the conventional isolation methods vs molecular identification procedure to detect Salmonella in broiler chicken meat has revealed that prevalence of Salmonella in meat samples was 12% in molecular method whereas it was 22% in the conventional method (Ibrahim et al, 2014). Use of PCR by targeting the sequences of hns and invA genes for rapid detection of Salmonella has been proven earlier too (El-Sebay et al, 2017). As a unique sequence to this genus available in fragment of invA gene, it has been verified as a precise PCR target (Rahn et al, 1992).…”
Section: Isolation Identification Confirmation and Serotyping Of Samentioning
Purpose : Salmonella infections continue to be a global problem with millions of humans and animal cases occurring annually. Broiler chicken plays a significant role causing Salmonella infections in Sri Lanka. Consumption of food contaminated with antimicrobial resistant Salmonella aggravates the problem. This study isolated, identified, and serotype the Salmonella spp. from broiler chicken meat in Sri Lanka and examined their antimicrobial susceptibility to be used in establishment of control measures. Research Method : Isolation of Salmonella species from broiler chicken meat was done by conventional method of isolation followed by polymerase chain reaction (PCR) confirmation. All PCR confirmed isolates of Salmonella were serotype and then, isolates were tested for antibiotic susceptibility using disc diffusion assay followed by the detection of antibiotic resistance genes using PCR. Findings : Broiler chicken meat in Sri Lanka is contaminated with Salmonella spp. at the prevalence of 11.6% and 8.9% of them carried hns and invA specific genes. Isolates were serotyped as Salmonella Typhimurium (47.8%), Salmonella Enteritidis (26.1%) and non typable (26.1%). Three isolates were resistant to ampicillin. Intermediate resistance was shown to three antibiotics and all the isolates were sensitive to nine antibiotics. Majority (56.5%) of Salmonella were sensitive to all the tested antibiotics. Prevalence of resistant genes for tetracycline, sulfonamides and aminoglycosides were within 4%-26%. None of the isolates except one (4%) carried chloramphenicol resistance genes. Originality / Value : Steps must be taken to minimize contamination of broiler chicken meat with Salmonella spp in Sri Lanka. Although, there is a low prevalence of antibiotic resistant isolates, its mere presence in broiler chicken is a warning signal of possibility of emergence of multidrug resistant strains.
“…detection as it contains sequences that are unique to the genus Salmonella . [34] Invasion A is a factor in the outer membrane of Salmonella spp. that is responsible for entering the host epithelial cells in the intestines thus initiating infection [34].…”
Section: Introductionmentioning
confidence: 99%
“…[34] Invasion A is a factor in the outer membrane of Salmonella spp. that is responsible for entering the host epithelial cells in the intestines thus initiating infection [34]. The inv locus in S. enterica serovar Typhimurium was characterized and it was reported that invA is essential in the display of virulence in the intestine [35].…”
Livestock are an important source of protein and food for humans, however opportunistic pathogens such as Salmonella spp. turn livestock into vehicles of foodborne diseases. This study investigated the prevalence of virulence genes in Salmonella spp. isolated from livestock production systems in two provinces of South Africa. During the period from May to August, 2018, a total of 361 faecal (189), oral (100), environmental (soil (36) and water (27)) and feed (9) samples were randomly collected from different animals (cattle, sheep, goats, pigs, ducks and chickens) that were housed in small-scale livestock production systems from Eastern Cape and KwaZulu-Natal Provinces in South Africa. Salmonella spp. were isolated and identified using microbiological and DNA molecular methods. Salmonella spp. were present in 29.0% of the samples of which 30.2% belonged to the Salmonella enterica species as confirmed by the positive amplification of the species specific iroB gene. Virulence genes that were screened from livestock-associated Salmonella were invA, iroB, spiC, pipD and int1. Statistically significant associations (p < 0.05) were established between the virulence genes, sampling location, animal host as well as the season when samples were collected. Furthermore, statistically significant (p < 0.05) positive correlations were observed between most of the virulence genes investigated. This is one of the recent studies to detect and investigate livestock-associated Salmonella spp. in South Africa. This study highlights the importance of continuous monitoring and surveillance for pathogenic salmonellae. It also demonstrated the detection and prevalence of virulent Salmonella spp. harbored by livestock in South Africa. This study demonstrated the potential risks of pathogenic Salmonella enterica to cause foodborne diseases and zoonotic infections from farm-to-fork continuum using the global one-health approach.
“…Salmonella is one of the most important foodborne pathogens which caused food safety hazards for the food industry. Salmonellosis is a critical medical issue and a major challenge worldwide having greater significance in developing countries [1]. The danger of Salmonella may vary between the production systems, caused by components of the husbandry systems affecting disease development and pathogen shedding or differences in the level of resistance to the pathogen [2].…”
Background and Aim: Salmonellosis is one of the most common foodborne bacterial diseases in the world. The great majority of Salmonella infections in humans are foodborne with Salmonella enterica and Salmonella Typhimurium accounting for a major part of the problem. The objective of this study was to investigate the presence of invA gene in strains of Salmonellae isolated from eggs and diarrheal swabs from human cases. In addition, the relationship between invA gene nucleotide sequences from different sources (human stool and egg samples) have been studied through phylogenetic tree.
Materials and Methods: One hundred and seventy eggs (eggshell and its contents) and 160 stool swabs samples were collected from four poultry farms and medical hospital in Giza Governorate.
Results: The study reported the presence of two Salmonella strains in eggshell surface with an overall isolation rate of 1.2 and 0% of the egg content. Salmonella Enteritidis and Salmonella Typhimurium were isolated from eggshell surface with an incidence of 50% for each strain. Six salmonella strains were isolated from human stool with an incidence of 3.75%; the isolated strains are S. Typhimurium, S. Enteritidis, Salmonella Virchow, Salmonella Haifa, and Salmonella Kentucky with an incidence of 33.3%, 16.6%, 16.6%, 16.6%, and 16.6%, respectively. Among eight Salmonella strains, invA gene was detected with percentage of 50%. The phylogenetic analysis of the sequences invA gene, from two isolates included in this study and five isolates retrieved from GenBank showed that sequence from human, layer hens, egg, and water in the same clusters.
Conclusion: Close relation between drinking contaminated water and layer hens and contaminated water is one such source.
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