Introgression of the high grain protein gene Gpc-B1 in an elite wheat variety of Indo-Gangetic Plains through marker assisted backcross breeding

Abstract

a b s t r a c tGrain protein content (GPC) in wheat has been a major trait of interest for breeders since it has enormous end use potential. In the present study, marker-assisted backcrossing (MABC) was successfully used to improve GPC in wheat cultivar HUW468. The genotype Glu269 was used as the donor parent for introgression of the gene Gpc-B1 that confers high GPC. In a segregating population, SSR marker Xucw108, with its locus linked to Gpc-B1 was used for foreground selection to select plants carrying Gpc-B1. Background selection, involving 86 polymorphic SSR markers dispersed throughout the genome, was exercised to recover the genome of HUW468. For eliminating linkage drag, markers spanning a 10 cM region around the gene Gpc-B1 were employed to select lines with a donor segment of the minimum size carrying the gene of interest. Improved lines had significantly higher GPC and displayed 88.4-92.3 per cent of the recurrent parent genome (RPG). For grain yield, selected lines were at par with the recurrent parent HUW468, suggesting that there was no yield penalty. The whole exercise of transfer of Gpc-B1 and reconstitution of the genome of HUW468 was completed within a period of two and half years (five crop cycles) demonstrating practical utility of MABC for developing high GPC lines in the background of any elite and popular wheat cultivar with relatively higher speed and precision.(http://creativecommons.org/licenses/by-nc-nd/3.0/).

Introgression of a gene for high grain protein content (Gpc-B1) into two leading cultivars of wheat in Eastern Gangetic Plains of India through marker assisted backcross breeding

“…The cultivar HUW 468 was also improved to see whether GPC can be improved through MABB using a donor different from that reported by Vishwakarma et al (2014).…”

“…In USA also, two hexaploid wheat cultivars (Lassik at University of California, Davis and Farnum at Washington State University, Pullman, USA) were developed using Gpc-B1 gene. Recently, a donor Glu-79 was used to successfully introgress Gpc-B1 gene in a wheat cultivar HUW468 (Vishwakarma et al, 2014) at Banaras Hindu University where the present investigation was conducted.This study was undertaken to improve GPC using MABB in a popular cultivar (HUW234) of EGP of India using a high GPC line 'PBW343+Gpc-B1+LR24' that contains Gpc-B1 gene linked to a known marker Xucw108. …”

“…Recently, a donor Glu-79 was used to successfully introgress Gpc-B1 gene in a wheat cultivar HUW468 (Vishwakarma et al, 2014) at Banaras Hindu University where the present investigation was conducted.…”

The Wheat Introgressive Form Evaluation by Grain Biochemical and Technological Properties

“…Благодаря разработке молекулярных маркеров, выявляю щих функционально активные и/или неактивные аллели Gpc-B1, стал возможен генетический скрининг образцов коллекций и ускоренный отбор из гиб-ридных популяций генотипов с определенным составом аллелей (Vishwakarma et al, 2014;Mishra et al, 2015). О возможности передачи генов высокого содержания бел-ка не только от T. dicoccum, но и от других образцов дикой двузернянки сообщалось в работах (Klindworth et al, 2009;Aykut Tonk et al, 2010;Hussein et al, 2014).…”

Properties of grain, flour and dough in bread wheat lines with Aegilops markgrafii introgressions

“…Its effect has been studied in different genetic backgrounds and in various environments (Kumar et al 2011;Tabbita et al 2013;Vishwakarma et al 2014). Thus, the gene can be used in breeding without any negative impact on yield.…”