Introduction to Tissue Engineering

Mhanna, Rami; Hasan, Anwarul

doi:10.1002/9783527689934.ch1

Cited by 27 publications

(27 citation statements)

References 144 publications

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“…The main approach in tissue engineering utilizes cells, signals and scaffolds for the design and construction of biological substitutes that should result in tissue regeneration [ 2 ]. Biomaterials are used as scaffolds for spatial distribution of specific cell types [ 3 ]. Biomaterials can potentially influence cell proliferation and extracellular matrix formation, both in positive and negative ways [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Assessment of a PCL-3D Printing-Dental Pulp Stem Cells Triplet for Bone Engineering: An In Vitro Study

et al. 2021

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The search of suitable combinations of stem cells, biomaterials and scaffolds manufacturing methods have become a major focus of research for bone engineering. The aim of this study was to test the potential of dental pulp stem cells to attach, proliferate, mineralize and differentiate on 3D printed polycaprolactone (PCL) scaffolds. A 100% pure Mw: 84,500 ± 1000 PCL was selected. 5 × 10 × 5 mm3 parallelepiped scaffolds were designed as a wood-pilled structure composed of 20 layers of 250 μm in height, in a non-alternate order ([0,0,0,90,90,90°]). 3D printing was made at 170 °C. Swine dental pulp stem cells (DPSCs) were extracted from lower lateral incisors of swine and cultivated until the cells reached 80% confluence. The third passage was used for seeding on the scaffolds. Phenotype of cells was determined by flow Cytometry. Live and dead, Alamar blue™, von Kossa and alizarin red staining assays were performed. Scaffolds with 290 + 30 μm strand diameter, 938 ± 80 μm pores in the axial direction and 689 ± 13 μm pores in the lateral direction were manufactured. Together, cell viability tests, von Kossa and Alizarin red staining indicate the ability of the printed scaffolds to support DPSCs attachment, proliferation and enable differentiation followed by mineralization. The selected material-processing technique-cell line (PCL-3D printing-DPSCs) triplet can be though to be used for further modelling and preclinical experiments in bone engineering studies.

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Section: Introductionmentioning

confidence: 99%

Assessment of a PCL-3D Printing-Dental Pulp Stem Cells Triplet for Bone Engineering: An In Vitro Study

et al. 2021

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“…Tissue engineering is one of the major components of regenerative medicine, which utilizes biomaterials, cells, and biochemical factors engineered together for tissue replacement . This technology helps to restore, maintain, or improve functions of tissues/organs that were damaged or lost due to injury, trauma, diseases, etc .…”

Section: Applicationsmentioning

confidence: 99%

Collagen from Marine Biological Sources and Medical Applications

Felician

Xia

et al. 2018

Chemistry & Biodiversity

202

140

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Collagen is the most studied protein with a wide range of applications including pharmaceutical, biomedical, cosmetics, leather, and film industries due to its special characteristics that are high biocompatibility, good bioactivity, and weak antigenicity. Although collagen sources are abundant, the outbreak of varied diseases among land animals posed threat to its utilization in our daily life. Thus, a probe for an alternative source began, which in turn revealed the immense untapped marine sources, such as fish, jellyfish, and some marine Mammals. The present article deals with a brief description of collagen, its characteristics, marine sources, extraction, collagen peptides and their biological activities, potential use and application in various field.

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“…Tissue engineering strategies combining material science, engineering, and life science will become new clinical therapeutic approaches in the future. Tissue engineering strategies contain three key elements: cells, scaffolds, and biomechanical or biochemical signals ( Mhanna and Hasan, 2017 ). These elements have been applied and extended in IVD tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Intervertebral Disk Degeneration: The Microenvironment and Tissue Engineering Strategies

Dou

Sun

et al. 2021

Front. Bioeng. Biotechnol.

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Intervertebral disk degeneration (IVDD) is a leading cause of disability. The degeneration is inevitable, and the mechanisms are complex. Current therapeutic strategies mainly focus on the relief of symptoms, not the intrinsic regeneration of the intervertebral disk (IVD). Tissue engineering is a promising strategy for IVDD due to its ability to restore a healthy microenvironment and promote IVD regeneration. This review briefly summarizes the IVD anatomy and composition and then sets out elements of the microenvironment and the interactions. We rationalized different scaffolds based on tissue engineering strategies used recently. To fulfill the complete restoration of a healthy IVD microenvironment, we propose that various tissue engineering strategies should be combined and customized to create personalized therapeutic strategies for each individual.

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Introduction to Tissue Engineering

Cited by 27 publications

References 144 publications

Assessment of a PCL-3D Printing-Dental Pulp Stem Cells Triplet for Bone Engineering: An In Vitro Study

Assessment of a PCL-3D Printing-Dental Pulp Stem Cells Triplet for Bone Engineering: An In Vitro Study

Collagen from Marine Biological Sources and Medical Applications

Intervertebral Disk Degeneration: The Microenvironment and Tissue Engineering Strategies

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