Introduction of DPR, an Enterostatin Fragment Peptide, into Soybean β-Conglycinin α′ Subunit by Site-directed Mutagenesis

Takenaka, Yasuyuki; Doyama, Naomi; Maruyama, Nobuyuki; Utsumi, Shigeru; Yoshikawa, Masaaki

doi:10.1271/bbb.68.253

Cited by 10 publications

(8 citation statements)

References 12 publications

(11 reference statements)

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“…The rapid hypocholesterolemic effect of DPR might be mediated, at least in part, by stimulation of bile acid secretion. In fact, DPR stimulated bile acid secretion in rats while VPDPR did not (Takenaka et al 2004;Shimizu 2004).…”

Section: Production Of Vpdpr Dpr Lpypr and Lplpr Peptides By Genetimentioning

confidence: 87%

“…The soluble fraction thus obtained showed ileum-contracting activity using longitudinal muscle strips of guinea pig (Takenaka et al 2001). Following the same approach Takenaka et al (2004), showed that the DPR sequence of the VPDPR fragment has hypocholesterolemic activity. The DPR sequence obtained by site directed mutagenesis, in the soybean b-conglycinin a-subunit, was expressed in E. coli and an accumulation of 15 % total protein was demonstrated.…”

Section: Production Of Vpdpr Dpr Lpypr and Lplpr Peptides By Genetimentioning

confidence: 98%

“…For example, Takenaka et al (2003) demonstrated that enterostatin did not increase excretion of bile acids in feces, suggesting that these peptides act by different mechanisms. It is proposed that this type of peptides may also bind to phospholipids and exert serum cholesterol lowering activity in humans (Takenaka et al 2000(Takenaka et al , 2004. In this section, we particularly focus on the functionality of lactostatin, since as of today it is the most studied peptide.…”

Section: Functionality Of Hypocholesterolemic Bioactive Peptidesmentioning

confidence: 99%

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Production and purification of recombinant hypocholesterolemic peptides

et al. 2014

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Elevated concentrations of cholesterol in plasma are associated with increased risk for heart diseases in humans. Bioactive peptides can be considered as an option to prevent or treat this condition. Currently, there are wide sources of bioactive peptides with hypocholesterolemic activities; however, most researches are focused in bioactive peptides derived from soybean and milk protein. Although there are several preparation methods for these peptides, it is a novel process to prepare bioactive peptides by genetic engineering techniques. In this review, after a general introduction on approaches and advances in bioactive peptides, recombinant strategies to generate hypocholesterolemic peptides and their purification are discussed as well as their application in food and drug design.

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Section: Production Of Vpdpr Dpr Lpypr and Lplpr Peptides By Genetimentioning

confidence: 87%

Section: Production Of Vpdpr Dpr Lpypr and Lplpr Peptides By Genetimentioning

confidence: 98%

Section: Functionality Of Hypocholesterolemic Bioactive Peptidesmentioning

confidence: 99%

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Production and purification of recombinant hypocholesterolemic peptides

et al. 2014

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“…This peptide has structural homology to enterostatin (Val-Pro-Asp-Pro-Arg). Although both have hypocholesterolemic activities, enterostatin did not increased excretion of bile acids in feces, suggesting that they may act by different mechanisms (Takenaka et al, 2004). Bioactivity of isoflavones.…”

Section: Improvement In Serum Lipid Profilementioning

confidence: 97%

“…For example, Leu-Pro-Tyr-Pro-Arg, a peptide from soybean glycinin A5A4B3 subunit (Takenaka et al, 2004) and Pro-Gly-Pro have been found to have anorectic activities. The consumption of soy protein may also lead to low hepatic deposits of triglycerides (Ascencio et al, 2004), reducing the risk of hepatic lipotoxicity and therefore of steatosis (Tovar et al, 2005).…”

Section: Effects On Obesity and Metabolic Syndromementioning

confidence: 99%