Design of genetically modified soybean proglycinin A1aB1b with multiple copies of bioactive peptide sequences

Comparative proteome analysis of seed storage and allergenic proteins among four narrow-leafed lupin cultivars

“…Most of the differentiating proteins were identified as b-conglutin, the major seed storage protein of lupin similar to 7s glubulins of soybean which has been investigated for some biological activity (Maruyama et al, 2003;Prak, Maruyama, Maruyama, & Utsumi, 2006). In contrast, c-conglutins having blood glucose lowering effect is the only lupin seed protein has been reported for individual bioactivity (Duranti et al, 2008) that showed similar expression among the studied cultivars.…”

Production and purification of recombinant hypocholesterolemic peptides

“…The change in pH can also be performed smoothly to recover different peptide groups. Nagaoka et al (2005) used weak anionexchange chromatography to isolate PHY, a polypeptide with hypocholesterolemic properties derived from Spirulina platensis and Prak et al (2006) used strong anion-exchange chromatography to purify sequences containing the hypocholesterolemic peptide IIAEK.…”

“…Zhong et al (2007a, b) also used gel filtration to separate peptide fractions with hypocholesterolemic activity. Similarly, Prak et al (2006) separated polypeptide fractions, with hypocholesterolemic activity, having the peptide sequence IIAEK. Alhaj et al (2010) used size-exclusion chromatography to separate a casein hydrolysate looking for peptides, below 1,200 Da, with cholesterol lowering activities.…”

Angiotensin-I-converting enzyme and prolyl endopeptidase inhibitory peptides from natural sources with a focus on marine processing by-products

“…Other methods include endogenous microbial activity of fermented foods such as cheese and yogurt (Je, Park, Jung, Park, & Kim, 2005). Also genetically modified proteins, such as the soybean proglycin A1aB1b, can be designed to carry multiple copies of bioactive sequences (Prak, Maruyama, Maruyama, & Utsumi, 2006).…”