Intratesticular Human Adipose-Derived Stem Cell (hADSC) Transplantation Decreased Oxidative Stress in Testicular Torsion Model of Wistar Rat

Siregar, Safendra; Noegroho, Bambang Sasongko; Adriansjah, Ricky; Mustafa, Akhmad; Wijayanti, Zola

doi:10.2147/rru.s283861

Cited by 6 publications

(3 citation statements)

References 20 publications

(21 reference statements)

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“…Moreover, compared with BMSCs, ADSCs can be harvested at a level of 100–1000 times the number of cells under the same extraction volume and can be a potential candidate for stem cell therapy [ 30 , 31 ]. In this study, we isolated ADSCs from human adipose tissue and used OA treatments for rats for the following reasons: (1) adipose tissue is an abundant source of hADSCs; (2) it has been reported that hADSCs do not express MHC class II, i.e., hADSCs do not trigger a xenogenic immunologic reaction [ 32 ]; and (3) the final goal of our OA treatment strategy is to use human autologous hADSCs to treat OA.…”

Section: Discussionmentioning

confidence: 99%

3D-Cultured Adipose-Derived Stem Cell Spheres Using Calcium-Alginate Scaffolds for Osteoarthritis Treatment in a Mono-Iodoacetate-Induced Rat Model

Lin

Kuan

Chang

et al. 2023

IJMS

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Osteoarthritis (OA) is a degenerative disease that causes pain, cartilage deformation, and joint inflammation. Mesenchymal stem cells (MSCs) are potential therapeutic agents for OA treatment. However, the 2D culture of MSCs could potentially affect their characteristics and functionality. In this study, calcium-alginate (Ca-Ag) scaffolds were prepared for human adipose-derived stem cell (hADSC) proliferation with a homemade functionally closed process bioreactor system; the feasibility of cultured hADSC spheres in heterologous stem cell therapy for OA treatment was then evaluated. hADSC spheres were collected from Ca-Ag scaffolds by removing calcium ions via ethylenediaminetetraacetic acid (EDTA) chelation. In this study, 2D-cultured individual hADSCs or hADSC spheres were evaluated for treatment efficacy in a monosodium iodoacetate (MIA)-induced OA rat model. The results of gait analysis and histological sectioning showed that hADSC spheres were more effective at relieving arthritis degeneration. The results of serological and blood element analyses of hADSC-treated rats indicated that the hADSC spheres were a safe treatment in vivo. This study demonstrates that hADSC spheres are a promising treatment for OA and can be applied to other stem cell therapies or regenerative medical treatments.

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Section: Discussionmentioning

confidence: 99%

3D-Cultured Adipose-Derived Stem Cell Spheres Using Calcium-Alginate Scaffolds for Osteoarthritis Treatment in a Mono-Iodoacetate-Induced Rat Model

Lin

Kuan

Chang

et al. 2023

IJMS

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“…Human adipose stem cells have low immunoantigenicity, due to their great commercial value, hADSC has been applied in animal experiments to expand their clinical adaptability [20,21]. We selected hADSC for injection and further explored it based on Schilling's study [11].…”

Section: Discussionmentioning

confidence: 99%

Human Adipose-Derived Stem Cells Delay Muscular Atrophy after Peripheral Nerve Injury in Rats

Wang

et al. 2022

Cell Biochem Biophys

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Introduction: Muscle is often accompanied by denervation atrophy after peripheral nerve injury. Human adipose-derived stem cells (hADSCs) and platelet-rich plasma injections into a muscle after peripheral nerve injury were examined.Methods: The rats were randomly divided into 5 groups. First group rats' sciatic nerve was exposed with untreated, others groups rats' sciatic nerve injury model was created rst. PBS, hADSCs, PRP, and a mixture of hADSCs and PRP were injected into the gastrocnemius immediately postoperatively. Quantitation of gross musculature and muscle ber area, SFI were investigated.Results: In 4 weeks post-surgery, post hoc Bonferroni tests showed signi cant differences in wet weight ratios when the hADSC group, the PRP group, and the combine group were compared with the PBS group.Areas of muscle ber were larger in the hADSC group and the combine group compared with the PBS group at 4 weeks post-surgery.Discussion: hADSCs injection may delay muscular atrophy after sciatic nerve injury in rats, and PRP injection has little effect on delayed muscular atrophy.

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“…The study of Siregar et al [ 166 ] established that hADSC protected the testis against I/R-induced oxidative stress. Also, Hsiao et al [ 167 ] revealed that Mesenchymal stem cells restored sperm quality and glycogenesis/glycolysis imbalance following I/R testicular injury via the modulation of Akt/GSK3 signaling.…”

Section: Anti-diabeticsmentioning

confidence: 99%

Pathophysiology and management of testicular ischemia/reperfusion injury: Lessons from animal models

Akhigbe,

Odetayo,

Akhigbe

et al. 2024

Heliyon

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Intratesticular Human Adipose-Derived Stem Cell (hADSC) Transplantation Decreased Oxidative Stress in Testicular Torsion Model of Wistar Rat

Cited by 6 publications

References 20 publications

3D-Cultured Adipose-Derived Stem Cell Spheres Using Calcium-Alginate Scaffolds for Osteoarthritis Treatment in a Mono-Iodoacetate-Induced Rat Model

3D-Cultured Adipose-Derived Stem Cell Spheres Using Calcium-Alginate Scaffolds for Osteoarthritis Treatment in a Mono-Iodoacetate-Induced Rat Model

Human Adipose-Derived Stem Cells Delay Muscular Atrophy after Peripheral Nerve Injury in Rats

Pathophysiology and management of testicular ischemia/reperfusion injury: Lessons from animal models

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