Human Adipose-Derived Stem Cells Delay Muscular Atrophy after Peripheral Nerve Injury in Rats

Abstract

Introduction: Muscle is often accompanied by denervation atrophy after peripheral nerve injury. Human adipose-derived stem cells (hADSCs) and platelet-rich plasma injections into a muscle after peripheral nerve injury were examined.Methods: The rats were randomly divided into 5 groups. First group rats' sciatic nerve was exposed with untreated, others groups rats' sciatic nerve injury model was created rst. PBS, hADSCs, PRP, and a mixture of hADSCs and PRP were injected into the gastrocnemius immediately postoperatively. Quantitation of gross musculature and muscle ber area, SFI were investigated.Results: In 4 weeks post-surgery, post hoc Bonferroni tests showed signi cant differences in wet weight ratios when the hADSC group, the PRP group, and the combine group were compared with the PBS group.Areas of muscle ber were larger in the hADSC group and the combine group compared with the PBS group at 4 weeks post-surgery.Discussion: hADSCs injection may delay muscular atrophy after sciatic nerve injury in rats, and PRP injection has little effect on delayed muscular atrophy.

Platelet Concentrates Preconditioning of Mesenchymal Stem Cells and Combined Therapies: Integrating Regenerative Strategies for Enhanced Clinical Applications

“…However, it is important to note that some studies have reported mixed results, with the combined treatment not consistently yielding significantly greater outcomes than individual treatments in certain scenarios. For example, muscle atrophy was observed in rats after superior sciatic nerve dissection following single or combined treatment 93 . In addition, in a 12-month follow-up study of knee OA from Brazil, researchers found that intra-articular injection of BMSCs, even without PRP, was effective in improving function and reducing symptoms caused by knee OA 106 .…”

Experimental study on the repair of peripheral nerve injuries via simultaneously coapting the proximal and distal ends of peripheral nerves to the side of nearby intact nerves

“…According to the severity and nature, peripheral nerve injury can be divided into nerve conduction dysfunction, nerve axon interruption and nerve rupture. After the peripheral nerve ruptures, pathological changes occur in nerve fibers, neuronal bodies and target organs (1)(2)(3). At present, common methods for repairing peripheral nerve injuries include neurolysis, nerve suture, nerve transplantation, nerve transfer and nerve implantation.…”