3D-Cultured Adipose-Derived Stem Cell Spheres Using Calcium-Alginate Scaffolds for Osteoarthritis Treatment in a Mono-Iodoacetate-Induced Rat Model

Lin, Yu Ying; Kuan, Che‐Yung; Chang, Chia-Tien; Chuang, Ming‐Hsi; Syu, Wan-Sin; Zhang, Kai-Ling; Lee, Chia-Hsin; Lin, Po Ting; Dong, Guo-Chung; Lin, Feng Huei

doi:10.3390/ijms24087062

Cited by 2 publications

(3 citation statements)

References 40 publications

(45 reference statements)

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“… 22 The isolation of ADSCs is minimally invasive and cells are easily isolated from subcutaneous fat. 84 Additionally, ADSCs have low immunogenic reactivity due to the limited expression of immunogenic antigens such as CD40, CD40L, CD80, and CD86. 85 A study conducted by Lin et al investigated the application of ADSCs in calcium-alginate hydrogel spheres and applied to a monosodium iodoacetate (MIA)-induced OA in vivo model in rats.…”

Section: Cell Therapies For Cartilage Regenerationmentioning

confidence: 99%

“…It was reported that the OA score was significantly reduced in the spheroid group as well. 84 A clinical study using the intra-articular injection of autologous ADSCs demonstrated an improvement in pain, function and mobility in patients with severe knee OA. 83 A meta-analysis comparing ADSCs and BM-MSCs found that ADSCs had a statistically significant and consistent improvement of outcome measurements compared to BM-MSCs.…”

Section: Cell Therapies For Cartilage Regenerationmentioning

confidence: 99%

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Cell Therapy Approaches for Articular Cartilage Regeneration

Makarczyk

2023

Organogenesis

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Articular cartilage is a common cartilage type found in a multitude of joints throughout the human body. However, cartilage is limited in its regenerative capacity. A range of methods have been employed to aid adults under the age of 45 with cartilage defects, but other cartilage pathologies such as osteoarthritis are limited to non-steroidal anti-inflammatory drugs and total joint arthroplasty. Cell therapies and synthetic biology can be utilized to assist not only cartilage defects but have the potential as a therapeutic approach for osteoarthritis as well. In this review, we will cover current cell therapy approaches for cartilage defect regeneration with a focus on autologous chondrocyte implantation and matrix autologous chondrocyte implantation. We will then discuss the potential of stem cells for cartilage repair in osteoarthritis and the use of synthetic biology to genetically engineer cells to promote cartilage regeneration and potentially reverse osteoarthritis.

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Section: Cell Therapies For Cartilage Regenerationmentioning

confidence: 99%

Section: Cell Therapies For Cartilage Regenerationmentioning

confidence: 99%

Cell Therapy Approaches for Articular Cartilage Regeneration

Makarczyk

2023

Organogenesis

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“…3D in vitro modelling is an important technique to investigate musculoskeletal conditions as it allows cell-cell and cell-ECM interactions in 3D, mimicking the complexity of physiological tissues [47]. The 3D culture models have been attempted for bone research, especially to represent bone diseases such as OA [48,49], OP [50,51], osteomyelitis [52,53], and cancer [53,54]. Such 3D culture models representing bone led to understanding the mechanism of action of factors such as irisin, an endogenous myokine secreted during sports, which has an anabolic effect [55].…”

Section: D Models and Organoids For The Bm 41 Current In Vitro Models...mentioning

confidence: 99%

Organoid Models and Next-Generation Sequencing for Bone Marrow and Related Disorders

Rausch

Iqbal

Pathak³

et al. 2023

Organoids

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Challenges to the musculoskeletal system negatively impact the quality of life of people suffering from them, leading to pain, a decline in mobility, genetic alterations, and potential disorders. The bone marrow (BM) forms an integral part of the musculoskeletal system responsible for erythropoiesis and optimal survival of the various immune and stem cells within the BM. However, due to its dynamic and complex three-dimensional (3D) structure, replicating the BM physiologically in traditional two-dimensional (2D) cell culture settings is often challenging, giving rise to the need for 3D in vitro models to better dissect the BM and its regeneration. Several researchers globally have been investigating various approaches to define an appropriate 3D model for their research. Organoids are novel preclinical models that provide a 3D platform for several tissues and have been analysed using next-generation sequencing (NGS) to identify new molecular pathways at the genetic level. The 3D in vitro models and organoids are increasingly considered important platforms for precision medicine. This review outlines the current knowledge of organoid and 3D in vitro models for the BM. We also discuss different types of 3D models and which may be more adaptable for the BM. Finally, we critically review the NGS techniques used for such models and the future combination of these techniques.

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3D-Cultured Adipose-Derived Stem Cell Spheres Using Calcium-Alginate Scaffolds for Osteoarthritis Treatment in a Mono-Iodoacetate-Induced Rat Model

Cited by 2 publications

References 40 publications

Cell Therapy Approaches for Articular Cartilage Regeneration

Cell Therapy Approaches for Articular Cartilage Regeneration

Organoid Models and Next-Generation Sequencing for Bone Marrow and Related Disorders

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