Intraoperative identification of esophageal sentinel lymph nodes with near-infrared fluorescence imaging

Parungo, Cherie P.; Ohnishi, Shunsuke; Kim, Sang–Wook; Kim, Sunjee; Laurence, Rita G.; Soltesz, Edward G.; Chen, Frederick Y.; Colson, Yolonda L.; Cohn, Lawrence H.; Bawendi, Moungi G.; Frangioni, John V.

doi:10.1016/j.jtcvs.2004.08.001

Cited by 176 publications

(110 citation statements)

References 20 publications

(3 reference statements)

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“…Optical imaging using NIR fluorescent lymphatic tracers provides real-time assessment of lymphatic flow from the point of injection to the SLN(s), relatively high sensitivity due to the penetrating ability of NIR photons, and elimination of the need for radioactivity. NIRFI has been validated in large animal models for SLN mapping of skin [16,17], lung [21], esophagus [22], pleural space [22], GI tract [16,21], and spontaneous melanoma [23]. Our study of invasive bladder cancer confirms that the anatomy of lymphatic flow is highly variable among individuals, and can involve structures such as the urachus and presacral nodes, and that SLN mapping must be performed on an individual basis.…”

Section: Discussionsupporting

confidence: 56%

Sentinel Lymph Node Mapping of Invasive Urinary Bladder Cancer in Animal Models Using Invisible Light

Knapp¹,

Adams²,

DeGrand

et al. 2007

European Urology

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Objectives-With conventional methodology, sentinel lymph node (SLN) mapping of invasive urinary bladder cancer is technically challenging. This study was performed to determine the utility of invisible, near-infrared fluorescent (NIRF) light for patient-specific SLN mapping, in real time under complete image guidance.Methods-Lymphatic tracers, injection volume, NIRF excitation fluence rate, light collection of emitted fluorescence, and degree of bladder distension were systematically optimized in normal dogs and pigs. SLN mapping was then performed in pet dogs with naturally occurring invasive transitional cell carcinoma (InvTCC) of the urinary bladder, which closely mimics the human disease.Results-NIRF albumin (hydrodynamic diameter [HD], 7.4 nm) and NIRF quantum dots (15-20 nm HD) injected into the bladder wall resulted in identification of draining lymph nodes (LNs) in under 3 min. In both species, considerable variability in the lymphatic drainage was observed among individuals. Optimal SLN mapping was achieved with the use of superficial, serosal injection of NIRF tracer, with the bladder distended to an intraluminal pressure of 20-40 cm H 2 O. In dogs with InvTCC, NIRF tracers identified SLNs that were confirmed histologically to harbor metastases.Conclusions-The use of invisible NIRF light permits real-time, patient-specific identification of SLNs that drain bladder cancer. Intraluminal bladder pressure is a key parameter that needs to be controlled for optimal results. KeywordsBladder cancer; Fluorescence; Intraoperative imaging; Sentinel lymph node mapping * Corresponding author. Purdue University, Department of Veterinary Clinical Sciences, Lynn Hall of Veterinary Medicine, 625 Harrison St, West Lafayette, IN 47907-2026. Tel.765-494-9900; Fax: 765-496-1108. E-mail address: knappd@purdue.edu (D.W. Knapp). Take-home message This article describes successful use of intraoperative near-infrared light to identify sentinel lymph nodes (SLNs) draining invasive urinary bladder cancer in animals. This technology will permit studies of a new approach to identify SLNs at the time of cystectomy.Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers

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Section: Discussionsupporting

confidence: 56%

Sentinel Lymph Node Mapping of Invasive Urinary Bladder Cancer in Animal Models Using Invisible Light

Knapp¹,

Adams²,

DeGrand

et al. 2007

European Urology

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“…Recently, there have been clinical imaging studies using the FDA-approved dye indocyanine green (ICG) for detection of sentinel lymph nodes in patients with gastric, 15 intrathoracic, 16,17 and lung cancer. 18 Surgical oncologists are currently investigating mapping regional metastasis by fluorescent optical imaging using ICG, noncovalently bound to human serum albumin.…”

Section: Discussionmentioning

confidence: 99%

Fluorescent labeled anti‐EGFR antibody for identification of regional and distant metastasis in a preclinical xenograft model

et al. 2008

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“…The recently developed cadmium telluride (Cd/Te) QDs emit fluorescence in the red and near-infrared range, which is ideal for in vivo imaging to avoid tissue auto-fluorescence. In fact, it has been applied to map sentinel lymph nodes in animal cancer models (Kim et al, 2004;Parungo et al, 2005;Soltesz et al, 2005Soltesz et al, , 2006Hama et al, 2007;Knapp et al, 2007).…”

mentioning

confidence: 99%

Fluorescent tumour imaging of type I IGF receptor in vivo: comparison of antibody-conjugated quantum dots and small-molecule fluorophore

Zhang

Zeng

et al. 2009

Br J Cancer

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BACKGROUND: The type I insulin-like growth factor receptor (IGF1R) is a transmembrane tyrosine kinase involved in cancer proliferation, survival, and metastasis. METHODS: In this study, we used two different fluorescent technologies (small-molecule fluorophores and quantum dot (QD) nanoparticles) to detect receptor expression and its downregulation by antibodies in vivo. RESULTS: After conjugation with AVE-1642, a humanised anti-IGF1R monoclonal antibody, both QDs (705 nm) or Alexa 680 (smallmolecule fluorophore) detected expression and downregulation of IGF1R in vitro. To examine their utility in vivo, either AVE-1642 conjugates were intravenously delivered to mice bearing xenograft tumours of mouse embryo fibroblasts expressing human IGF1R or MCF-7 human breast cancer cells. Quantum dot fluorescence was mainly localised to the reticuloendothelial system in several organs and engulfed by macrophages, with only very small amount of QDs detected in the xenograft tumours. Depletion of macrophages by clodronate liposomes did not alter the nonspecific uptake of QDs. In contrast, AVE-1642-conjugated Alexa 680 solely targeted to xenograft tumour and was able to detect IGF1R downregulation, with little nonspecific targeting to other tissues or organs in mice. CONCLUSION: Taken together, our data suggest that small-molecule fluorophores, not QDs, are suitable to detect the expression and downregulation of IGF1R in vivo.

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Intraoperative identification of esophageal sentinel lymph nodes with near-infrared fluorescence imaging

Abstract: Near-infrared fluorescence imaging of sentinel lymph nodes is a novel and reliable intraoperative technique with the power to assist with identification and resection of esophageal sentinel lymph nodes.

Cited by 176 publications

References 20 publications

Sentinel Lymph Node Mapping of Invasive Urinary Bladder Cancer in Animal Models Using Invisible Light

Sentinel Lymph Node Mapping of Invasive Urinary Bladder Cancer in Animal Models Using Invisible Light

Fluorescent labeled anti‐EGFR antibody for identification of regional and distant metastasis in a preclinical xenograft model

Fluorescent tumour imaging of type I IGF receptor in vivo: comparison of antibody-conjugated quantum dots and small-molecule fluorophore

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