Fluorescent labeled anti‐EGFR antibody for identification of regional and distant metastasis in a preclinical xenograft model

Gleysteen, John P.; Newman, J. Robert; Chhieng, David C.; Frost, Andra R.; Zinn, Kurt R.; Rosenthal, Eben L.

doi:10.1002/hed.20782

Cited by 61 publications

(68 citation statements)

References 20 publications

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“…Gleysteen et al conjugated Cetuximab to Cy5.5 and used it in SCID mice in a pulmonary metastatic model as well as a model of regional head and neck metastases. 72 In this study, the fluorescence was successfully confirmed by histopathology in all mice.…”

Section: Article In Presssupporting

confidence: 59%

The use of fluorescent dyes and probes in surgical oncology

Velde

Veerman

Subramaniam

et al. 2010

European Journal of Surgical Oncology (EJSO)

130

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Aims and background: Improved visualization of surgical targets inside of the patient helps to improve radical resection of the tumor while sparing healthy surrounding tissue. In order to achieve an image, optical contrast must be generated by properties intrinsic to the tissue, or require the attachment of special visualization labels to the tumor. In this overview the current status of the clinical use of fluorescent dyes and probes are reviewed. Methods:In this review, all experimental and clinical studies concerning fluorescent imaging were included. In addition, in the search for the optimal fluorescent imaging modality, all characteristics of a fluorescent dye were described. Findings and Conclusions:Although the technique of imaging through fluorescence sounds promising and several animal models show efficacy, official approval of these agents for further clinical evaluation, is eagerly awaited.

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Section: Article In Presssupporting

confidence: 59%

The use of fluorescent dyes and probes in surgical oncology

Velde

Veerman

Subramaniam

et al. 2010

European Journal of Surgical Oncology (EJSO)

130

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“…In order to detect tumors using NIR fluorescence, contrast agents that target tumor-specific characteristics can be used to selectively label tumor cells [16,17,18,19]. Novel NIR fluorescent agents have been developed that target tumor-specific cell surface markers [16,20], enzymatic activity [17,19,21] or increased glucose metabolism [22]. However, these tumor-specific agents are not yet available for clinical use.…”

Section: Introductionmentioning

confidence: 99%

Near-Infrared Fluorescence Imaging in Patients Undergoing Pancreaticoduodenectomy

Hutteman¹,

Vorst²,

Mieog³

et al. 2011

Eur Surg Res

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Background: Intraoperative visualization of pancreatic tumors has the potential to improve radical resection rates. Intraoperative visualization of the common bile duct and bile duct anastomoses could be of added value. In this study, we explored the use of indocyanine green (ICG) for these applications and attempted to optimize injection timing and dose. Methods: Eight patients undergoing a pancreaticoduodenectomy were injected intravenously with 5 or 10 mg ICG. During and after injection, the pancreas, tumor, common bile duct and surrounding organs were imaged in real time using the Mini-FLARE™ near-infrared (NIR) imaging system. Results: No clear tumor-to-pancreas contrast was observed, except for incidental contrast in 1 patient. The common bile duct was clearly visualized using NIR fluorescence, within 10 min after injection, with a maximal contrast between 30 and 90 min after injection. Patency of biliary anastomoses could be visualized due to biliary excretion of ICG. Conclusion: No useful tumor demarcation could be visualized in pancreatic cancer patients after intravenous injection of ICG. However, the common bile duct and biliary anastomoses were clearly visualized during the observation period. Therefore, these imaging strategies could be beneficial during biliary surgery in cases where the surgical anatomy is aberrant or difficult to identify.

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“…We opted for oral cavity implantation of tumor fragments rather than directly injecting tumor cells [5]. In other orthotopic models described in the literature, cell injection may induce dissemination of cancer cells by the hydrostatic pressure from the syringe during injection, resulting in the rapid development of large tumors, requiring sacrifice and thus making it difficult for lymph-node metastasis to develop [7][8][9]. In certain orthotopic animal HNSCC models using cell injection, the cervical lymph-node metastases that did develop may have been due to cell dissemination by hydrostatic pressure in the lymph vessels during injection rather than to cell migration from the primary site [9,10].…”

Section: Discussionmentioning

confidence: 99%

Role of near-infrared fluorescence imaging in the resection of metastatic lymph nodes in an optimized orthotopic animal model of HNSCC

Atallah

Milet

Quatre

et al. 2015

European Annals of Otorhinolaryngology, Head and Neck Diseases

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Fluorescent labeled anti‐EGFR antibody for identification of regional and distant metastasis in a preclinical xenograft model

Abstract: Background-Detection of regional and distant metastatic disease has significant implications for patient management. Fluorescent imaging may be a useful technique for metastasis detection and removal.

Cited by 61 publications

References 20 publications

The use of fluorescent dyes and probes in surgical oncology

The use of fluorescent dyes and probes in surgical oncology

Near-Infrared Fluorescence Imaging in Patients Undergoing Pancreaticoduodenectomy

Role of near-infrared fluorescence imaging in the resection of metastatic lymph nodes in an optimized orthotopic animal model of HNSCC

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