Role of near-infrared fluorescence imaging in the resection of metastatic lymph nodes in an optimized orthotopic animal model of HNSCC

Abstract

Near infrared fluorescence imaging provides real-time contrast between normal and malignant tissue, allowing intraoperative detection of metastatic lymph nodes. This preclinical stage is essential before testing the technique in humans.

RGD-Binding Integrins in Head and Neck Cancers

“…This orthotopic model allows long term follow up of mice, but the CAL33 tumour cell line used was highly αvβ3-expressing which does not represent HNSCC clinical integrin expression profiles [123]. The same group has used intraoperative αvβ3 imaging to improve the resection of lymph node metastases [124]. An indocyanine green-labelled αvβ6 antibody has also been proposed to improve resection margins by detecting tumour-specific expression at the invading tumour edge [118].…”

Gold nanoclusters as a contrast agent for image-guided surgery of head and neck tumors

“…In this context, nanotechnology might bring a strong support to overcome these issues, especially in the field image-guided surgery (2,3). When the surgery is assisted by real-time imaging, the tumor resection might be improved with the removal of small residues in particular and sufficient margin, the healthy tissues might be spared, and the time of surgery might be reduced (4)(5)(6). NIR imageguided surgery has already been validated in small animal studies (5,7) and is currently under investigation in clinical trials.…”

The feasibility of folate receptor alpha- and HER2-targeted intraoperative fluorescence-guided cytoreductive surgery in women with epithelial ovarian cancer: A systematic review

“…To accomplish this, the targeting component can be changed to one less likely to cross-react with other targets or different imaging agents with multiple targets can be combined. For example, metastatic lymph nodes could be detected with fluorescence imaging in animal models of head and neck cancer using the αvβ3 integrin as a target [43]. This integrin is also overexpressed in some EOCs and shows promising results as a target in FGCS on EOC animal-models [44][45][46].…”