Intraepithelial Lymphocytes in the Duodenal Mucosa of Meat Quails Receiving Diets Containing or Not Containing Probiotic and Different Levels of Crude Protein

Abstract

ZARDO, A.; OTUTUMI, L. K.; MEZALIRA, T. S.; FERREIRA, F. A.; CESTARI, I. E. D.; PREVIATO DO AMARAL, P. F. G.; SILVEIRA, A. P. Intraepithelial lymphocytes in the duodenal mucosa of meat quails receiving diets containing or not probiotic and different levels of crude protein. Arq. Ciênc. Vet. Zool. UNIPAR, Umuarama, v.18, n.2, p. 83-86, abr./jun. 2015. ABSTRACT:The aim of this study is to evaluate the effect of probiotic supplementation associated to different levels of crude protein (CP) on the count of intraepithelial lymphocytes (IELs) in the duodenum of meat quails. A total of 2304 quails were distributed in a completely randomized design in a 2 x 4 factorial scheme (with and without probiotic and four levels of CP -15, 20, 25 and 30%), with two replicates per treatment in two experimental periods, in a total of 32 experimental units. At seven days old, two quails from each experimental unit were euthanized to harvest the duodenum segment. Semi-serial 7-μm histological sections were obtained, which were subsequently stained with hematoxylin-eosin to perform the IEL count. For the calculation, first 2500 epithelial cells were counted from the mucosa of each animal, and then the IELs present between these cells were counted, with the results expressed in amounts of IELs/100 epithelial cells. No differences were found in the IEL count among the treatments. Under the experimental conditions, it can be concluded that the use of probiotic associated to different levels of CP supplementation does not alter the IEL count in the duodenum of meat quails. LINFÓCITOS INTRAEPITELIAISNA MUCOSA DO DUODENO DE CODORNAS DE CORTE RECEBENDO DIETAS CONTENDO OU NÃO PROBIÓTICO E DIFERENTES NÍVEIS DE PROTEÍNA BRUTA RESUMO: O objetivo deste trabalho foi avaliar o efeito da suplementação de probiótico associado com diferentes níveis de proteína bruta (PB) sobre a contagem de linfócitos intraepiteliais (LIEs) no duodeno de codornas de corte. Um total de 2304 codornas foram distribuídas em um delineamento inteiramente casualizado em esquema fatorial 2 x 4 (com e sem probiótico e quatro níveis de PB -15, 20, 25 e 30%), com duas repetições por tratamento em dois períodos experimentais, totalizando 32 unidades experimentais. Aos sete dias de idade, duas codornas de cada unidade experimental foi eutanasiada para colheita do segmento do duodeno. Cortes histológicos semi-seriados de sete μm foram obtidos, os quais foram posteriormente corados com hematoxilina-eosina para realização da contagem de LIEs. Para o cálculo, primeiro foram contados 2500 células epiteliais da mucosa de cada animal, e então os LIEs presentes entre essas células foram contados e os resultados expressos como quantidade de LIEs /100 células epiteliais. Não foram encontradas diferenças entre os tratamentos na contagem de LIEs. Nas condições em que o experimento foi desenvolvido, conclui-se que o uso de probiótico associado a diferentes níveis de PB não altera a contagem de LIEs do duodeno de codornas de corte. PALAVRAS-CHAVE: Aves. Enterócitos. Linfócitos. Mucosa intestinal...

Effect of crude protein reduction in blood, performance, immunological, and intestinal histological parameters of broiler chickens

“…We also found different estimate levels for CP requirements for maximum response of CD8+TCRVβ1‐, which represents peripheral cytotoxic cells, indicating differences in peripheral and mucosal immunity in healthy animals (as CD8 + TCRVβ1 + presented a trend to an increased requirement). This was observed in quails, which no effect on the amount of intestinal intraepithelial lymphocytes could be observed, probably reflecting the fact that mucosal immunity responds to stimuli quite differently from the peripheral immune system (Zardo et al, 2015). Also, CD8+CD28+ presented the highest requirements.…”

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