Intracellular Temperature Sensing: An Ultra-bright Luminescent Nanothermometer with Non-sensitivity to pH and Ionic Strength

Liu, Helin; Fan, Yanyan; Wang, Jianhai; Song, Zhongsen; Shi, Hao; Ren, Han; Yan, Sha; Jiang, Yuqiang

doi:10.1038/srep14879

Cited by 59 publications

(43 citation statements)

References 31 publications

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“…NPs have been developed to respond to a diverse range of cues, for instance including use of carbon dots to detect intracellular copper ions [345] and Qdot-encapsulated polymer to monitor temperature [346]; however, the majority of environmentally responsive systems have been directed towards measuring local pH, oxygen, and activities of enzymes such as proteases.…”

Section: The Nuts and Bolts Of Ivm Nanoparticle Imagingmentioning

confidence: 99%

Imaging the pharmacology of nanomaterials by intravital microscopy: Toward understanding their biological behavior

Miller

Weissleder

2017

Advanced Drug Delivery Reviews

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Therapeutic nanoparticles (NPs) can deliver cytotoxic chemotherapeutics and other drugs more safely and efficiently to patients; furthermore, selective delivery to target tissues can theoretically be accomplished actively through coating NPs with molecular ligands, and passively through exploiting physiological “enhanced permeability and retention” features. However, clinical trial results have been mixed in showing improved efficacy with drug nano-encapsulation, largely due to heterogeneous NP accumulation at target sites across patients. Thus, a clear need exists to better understand why many NP strategies fail in vivo and not result in significantly improved tumor uptake or therapeutic response. Multicolor in vivo confocal fluorescence imaging (intravital microscopy; IVM) enables integrated pharmacokinetic and pharmacodynamic (PK/PD) measurement at the single-cell level, and has helped answer key questions regarding the biological mechanisms of in vivo NP behavior. This review summarizes progress to date and also describes useful technical strategies for successful IVM experimentation.

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Section: The Nuts and Bolts Of Ivm Nanoparticle Imagingmentioning

confidence: 99%

Imaging the pharmacology of nanomaterials by intravital microscopy: Toward understanding their biological behavior

Miller

Weissleder

2017

Advanced Drug Delivery Reviews

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“…Among the non-invasive thermometric methods, luminescence thermometry has been established as the key alternative and an accurate technique with high detection sensitivity, spatial resolution and short acquisition times567. The temperature sensors using luminescent materials are based on the change in photoluminescence properties as a function of temperatures.…”

mentioning

confidence: 99%

“…During the past decade, abundant research has been carried out based on organic dyes and QD thermometry. Nano-thermometers based on rare earth doped hosts with wide band gap have also been reported123456789101112. According to previous reports on luminescence thermometry, the temperature sensing via the measurements of emission lifetime requires more complex equipment.…”

mentioning

confidence: 99%

“…According to previous reports on luminescence thermometry, the temperature sensing via the measurements of emission lifetime requires more complex equipment. Regarding the luminescence thermometric applications, the FIR technique is found to be faster and simpler than the luminescence lifetime method123456789101112. Recently, increased interest is focused on developing thermometers using wide band gap hosts doped with suitable rare earth ions via the FIR method.…”

mentioning

confidence: 99%

“…The performance of luminescent thermometers significantly depends on the photoluminescence efficiency of the corresponding luminescent materials123456789101112. Luminescent materials with different microstructures can be synthesized via different methods13.…”

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confidence: 99%

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Structural evaluations and temperature dependent photoluminescence characterizations of Eu3+-activated SrZrO3 hollow spheres for luminescence thermometry applications

Das

Som

Yang

et al. 2016

Sci Rep

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This research is focused on the temperature sensing ability of perovskite SrZrO 3 :Eu 3+ hollow spheres synthesized via the sol-gel method followed by heating. The Rietveld refinement indicated that the precursors annealed at 1100 °C were crystallized to form orthorhombic SrZrO 3 . SrZrO 3 particles exhibited non-agglomerated hollow spherical morphology with an average particle size of 300 nm. The UV-excited photoluminescence spectrum of SrZrO 3 :Eu 3+ consisted of two regions. One region was associated with SrZrO 3 trap emission, and the other one was related to the emission of Eu 3+ ions. The intensity ratio of the emission of Eu 3+ ions to the host emission (FIR) and the emission lifetime of Eu 3+ ions were measured in the temperature range of 300-550 K. The sensitivity obtained via the lifetime method was 7.3× lower than that measured via the FIR. Within the optimum temperature range of 300-460 K, the as-estimated sensor sensitivity was increased from 0.0013 to 0.028 K −1 . With a further increase in temperatures, the sensitivity started to decline. A maximum relative sensitivity was estimated to be 2.22%K −1 at 460 K. The resolutions in both methods were below 1K in the above temperature range. The results indicated the suitability of SrZrO 3 :Eu 3+ for the distinct high temperature sensing applications.In daily human life, temperature is recognized as one of the most important measured physical quantities. An accurate and consistent measurement of temperature is essential for the devices related to chemistry, medicine, biology and metrology owing to the cooling and heating phenomena. In the modern world, temperature sensors are widely used, accounting for about 75-80% of the overall sensor market. The conventional temperature sensing measurement mainly depends on the ability of materials in response to heat. Direct contact of the instrument with the heated objects is the main requirement for making such thermometric measurements [1][2][3] . Nowadays, non-contact specific thermometry with high resolution for sensing temperatures in nanoscale environments has emerged as a very dynamic field of research 4 . Among the non-invasive thermometric methods, luminescence thermometry has been established as the key alternative and an accurate technique with high detection sensitivity, spatial resolution and short acquisition times [5][6][7] . The temperature sensors using luminescent materials are based on the change in photoluminescence properties as a function of temperatures. The photoluminescence properties include the emission intensity, peak position, full width at half maxima of the emission spectrum and the characteristic lifetime of the excited state 8,9 .

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Spotlight on Luminescence Thermometry: Basics, Challenges, and Cutting‐Edge Applications

et al. 2023

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Luminescence (nano)thermometry is a remote sensing technique that relies on the temperature dependency of the luminescence features (e.g., bandshape, peak energy or intensity, and excited state lifetimes and risetimes) of a phosphor to measure temperature. This technique provides precise thermal readouts with superior spatial resolution in short acquisition times. Although luminescence thermometry is just starting to become a more mature subject, it exhibits enormous potential in several areas, e.g., optoelectronics, photonics, micro‐ and nanofluidics, and nanomedicine. This work reviews the latest trends in the field, including the establishment of a comprehensive theoretical background and standardized practices. The reliability, repeatability, and reproducibility of the technique are also discussed, along with the use of multiparametric analysis and artificial‐intelligence algorithms to enhance thermal readouts. In addition, examples are provided to underscore the challenges that luminescence thermometry faces, alongside the need for a continuous search and design of new materials, experimental techniques, and analysis procedures to improve the competitiveness, accessibility, and popularity of the technology

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Intracellular Temperature Sensing: An Ultra-bright Luminescent Nanothermometer with Non-sensitivity to pH and Ionic Strength

Cited by 59 publications

References 31 publications

Imaging the pharmacology of nanomaterials by intravital microscopy: Toward understanding their biological behavior

Imaging the pharmacology of nanomaterials by intravital microscopy: Toward understanding their biological behavior

Structural evaluations and temperature dependent photoluminescence characterizations of Eu3+-activated SrZrO3 hollow spheres for luminescence thermometry applications

Spotlight on Luminescence Thermometry: Basics, Challenges, and Cutting‐Edge Applications

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