Imaging the pharmacology of nanomaterials by intravital microscopy: Toward understanding their biological behavior

Miller, Miles A.; Weissleder, Ralph

doi:10.1016/j.addr.2016.05.023

Cited by 60 publications

(41 citation statements)

References 402 publications

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“…IVM also allows determination of a response to therapeutic intervention, for example by measuring response to angiogenesis inhibitors such as α-VEGFR antibodies [86]. In peritoneal research, due to the superficial localization of microvessels, high-definition IVM may eventually allow for greater mechanistic understanding of transport processes, particularly extravasation and the role of movement through intercellular gaps, transendothelial cell pores, and transcytosis (reviewed in [87]). …”

Section: Enhanced Permeability and Retention (Epr)mentioning

confidence: 99%

Functional vascular anatomy of the peritoneum in health and disease

Solaß

Horvath

Struller

et al. 2019

Pleura and Peritoneum

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Abstract:The peritoneum consists of a layer of mesothelial cells on a connective tissue base which is perfused with circulatory and lymphatic vessels. Total effective blood flow to the human peritoneum is estimated between 60 and 100 mL/min, representing 1-2 % of the cardiac outflow. The parietal peritoneum accounts for about 30 % of the peritoneal surface (anterior abdominal wall 4 %) and is vascularized from the circumflex, iliac, lumbar, intercostal, and epigastric arteries, giving rise to a quadrangular network of large, parallel blood vessels and their perpendicular offshoots. Parietal vessels drain into the inferior vena cava. The visceral peritoneum accounts for 70 % of the peritoneal surface and derives its blood supply from the three major arteries that supply the splanchnic organs, celiac and superior and inferior mesenteric. These vessels give rise to smaller arteries that anastomose extensively. The visceral peritoneum drains into the portal vein. Drugs absorbed are subject to firstpass hepatic metabolism. Peritoneal inflammation and cancer invasion induce neoangiogenesis, leading to the development of an important microvascular network. Anatomy of neovessels is abnormal and characterized by large size, varying diameter, convolution and blood extravasation. Neovessels have a defective ultrastructure: formation of large "mother vessels" requires degradation of venular and capillary basement membranes. Mother vessels give birth to numerous "daughter vessels". Diffuse neoangiogenesis can be observed before appearance of macroscopic peritoneal metastasis. Multiplication of the peritoneal capillary surface by neoangiogenesis surface increases the part of cardiac outflow directed to the peritoneum.

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Section: Enhanced Permeability and Retention (Epr)mentioning

confidence: 99%

Functional vascular anatomy of the peritoneum in health and disease

Solaß

Horvath

Struller

et al. 2019

Pleura and Peritoneum

View full text Add to dashboard Cite

show abstract

“…For example, positron emission tomography (PET) imaging in the clinic can guide tumour biopsies, deliver drugs via image guidance, and detect therapeutic response in the absence of tumour shrinkage 129,146 . In research settings, intravital microscopy is used to study drug pharmacokinetics and pharmacodynamics using fluorescent companion imaging drugs 147–149 , combined with imaging of orthotopic tumour xenograft mouse models and methods to study drug targeting 150 .…”

Section: Cellular and Molecular Imagingmentioning

confidence: 99%

Engineering and physical sciences in oncology: challenges and opportunities

2017

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The principles of engineering and physics have been applied to oncology for nearly 50 years. Engineers and physical scientists have made contributions to all aspects of cancer biology, from quantitative understanding of tumour growth and progression to improved detection and treatment of cancer. Many early efforts focused on experimental and computational modelling of drug distribution, cell cycle kinetics and tumour growth dynamics. In the past decade, we have witnessed exponential growth at the interface of engineering, physics and oncology that has been fuelled by advances in fields including materials science, microfabrication, nanomedicine, microfluidics, imaging, and catalysed by new programmes at the National Institutes of Health (NIH), including the National Institute of Biomedical Imaging and Bioengineering (NIBIB), Physical Sciences in Oncology, and the National Cancer Institute (NCI) Alliance for Nanotechnology. Here, we review the advances made at the interface of engineering and physical sciences and oncology in four important areas: the physical microenvironment of the tumour and technological advances in drug delivery; cellular and molecular imaging; and microfluidics and microfabrication. We discussthe research advances, opportunities and challenges for integrating engineering and physical sciences with oncology to develop new methods to study, detect and treat cancer, and we also describe the future outlook for these emerging areas.

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“…Currently, the widely used imaging modalities include magnetic resonance imaging, computerized tomography, and whole‐body fluorescence imaging, which usually provide “bulk” picture showing the morphology and size changes of tumor rather than dynamic structures and activities within tumors . For imaging and monitoring the tumor vasculature structures, intravital fluorescence microscopy with cellular resolution and high sensitivity can be applied . Specifically, two‐photon fluorescence microscopy (2PFM) enables noninvasive and real‐time imaging with intrinsic 3D optical sections of living subjects .…”

Section: Methodsmentioning

confidence: 99%

NIR‐II‐Excited Intravital Two‐Photon Microscopy Distinguishes Deep Cerebral and Tumor Vasculatures with an Ultrabright NIR‐I AIE Luminogen

et al. 2019

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Intravital fluorescence imaging of vasculature morphology and dynamics in the brain and in tumors with large penetration depth and high signal‐to‐background ratio (SBR) is highly desirable for the study and theranostics of vascular‐related diseases and cancers. Herein, a highly bright fluorophore (BTPETQ) with long‐wavelength absorption and aggregation‐induced near‐infrared (NIR) emission (maximum at ≈700 nm) is designed for intravital two‐photon fluorescence (2PF) imaging of a mouse brain and tumor vasculatures under NIR‐II light (1200 nm) excitation. BTPETQ dots fabricated via nanoprecipitation show uniform size of around 42 nm and a high quantum yield of 19 ± 1% in aqueous media. The 2PF imaging of the mouse brain vasculatures labeled by BTPETQ dots reveals a 3D blood vessel network with an ultradeep depth of 924 µm. In addition, BTPETQ dots show enhanced 2PF in tumor vasculatures due to their unique leaky structures, which facilitates the differentiation of normal blood vessels from tumor vessels with high SBR in deep tumor tissues. Moreover, the extravasation and accumulation of BTPETQ dots in deep tumor (more than 900 µm) is visualized under NIR‐II excitation. This study highlights the importance of developing NIR‐II light excitable efficient NIR fluorophores for in vivo deep tissue and high contrast tumor imaging.

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Imaging the pharmacology of nanomaterials by intravital microscopy: Toward understanding their biological behavior

Cited by 60 publications

References 402 publications

Functional vascular anatomy of the peritoneum in health and disease

Functional vascular anatomy of the peritoneum in health and disease

Engineering and physical sciences in oncology: challenges and opportunities

NIR‐II‐Excited Intravital Two‐Photon Microscopy Distinguishes Deep Cerebral and Tumor Vasculatures with an Ultrabright NIR‐I AIE Luminogen

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