Intracellular scavenging activity of Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid) in the fission yeast, Schizosaccharomyces pombe

Hamad, İsmail; Arda, Nazlı; Pekmez, Murat; Karaer, Semian; Temizkan, Güler

doi:10.4103/0976-9668.71667

Cited by 46 publications

(44 citation statements)

References 25 publications

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“…MitoVitE contains a 2-carbon chain which links the functional chromanol moiety of vitamin E to a TPP cation and is able to cross the outer mitochondrial membrane due to the positive charge of the TPP conjugate [6,30]. In Trolox, the chromanol is connected to a short-chain carboxylic acid making Trolox hydrophilic and cell-permeable such that it accumulates in the cell cytosol [6][7][8][9]. MitoVitE is more effective than Trolox both in vitro and in vivo [6,31].…”

Section: Discussionmentioning

confidence: 99%

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Differential Effects of MitoVitE, α-Tocopherol and Trolox on Oxidative Stress, Mitochondrial Function and Inflammatory Signalling Pathways in Endothelial Cells Cultured under Conditions Mimicking Sepsis

et al. 2020

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Sepsis is a life-threatening response to infection associated with inflammation, oxidative stress and mitochondrial dysfunction. We investigated differential effects of three forms of vitamin E, which accumulate in different cellular compartments, on oxidative stress, mitochondrial function, mRNA and protein expression profiles associated with the human Toll-like receptor (TLR) -2 and -4 pathways. Human endothelial cells were exposed to lipopolysaccharide (LPS)/peptidoglycan G (PepG) to mimic sepsis, MitoVitE, α-tocopherol, or Trolox. Oxidative stress, mitochondrial function, mitochondrial membrane potential and metabolic activity were measured. NFκB-P65, total and phosphorylated inhibitor of NFκB alpha (NFκBIA), and STAT-3 in nuclear extracts, interleukin (IL)-6 and IL-8 production in culture supernatants and cellular mRNA expression of 32 genes involved in Toll-like receptor-2 and -4 pathways were measured. Exposure to LPS/PepG caused increased total radical production (p = 0.022), decreased glutathione ratio (p = 0.016), reduced membrane potential and metabolic activity (both p < 0.0001), increased nuclear NFκB-P65 expression (p = 0.016) and increased IL-6/8 secretion (both p < 0.0001). MitoVitE, α- tocopherol and Trolox were similar in reducing oxidative stress, NFκB activation and interleukin secretion. MitoVitE had widespread downregulatory effects on gene expression. Despite differences in site of actions, all forms of vitamin E were protective under conditions mimicking sepsis. These results challenge the concept that protection inside mitochondria provides better protection.

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Section: Discussionmentioning

confidence: 99%

“…Trolox (6-hydroxy-2,5,7,8-tetra-methylchroman-2-carboxylic acid) is a synthetic, water soluble and cell-permeable derivative of vitamin E which accumulates in the cell cytosol. It is a potent antioxidant in several model systems [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Differential Effects of MitoVitE, α-Tocopherol and Trolox on Oxidative Stress, Mitochondrial Function and Inflammatory Signalling Pathways in Endothelial Cells Cultured under Conditions Mimicking Sepsis

et al. 2020

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“…These antioxidative effects may be due to the ability of these compounds to act as radical trapping agents [25,26], and the capacity of the organotellurium compound to mimic the action of the GPx-enzymes [32]. A previous study has shown that Trolox is likely to exert protection against intracellular H 2 O 2 by intracellular scavenging [40]. In our experimental model, the cells were exposed to the antioxidants and peroxide at the same time, which seems to indicate that it is the extracellular scavenging of peroxide which confers the protection seen with C1.…”

Section: Discussionmentioning

confidence: 99%

Selenium- and Tellurium-Based Antioxidants for Modulating Inflammation and Effects on Osteoblastic Activity

Mestres

Singh

et al. 2017

Antioxidants

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Increased oxidative stress plays a significant role in the etiology of bone diseases. Heightened levels of H2O2 disrupt bone homeostasis, leading to greater bone resorption than bone formation. Organochalcogen compounds could act as free radical trapping agents or glutathione peroxidase mimetics, reducing oxidative stress in inflammatory diseases. In this report, we synthesized and screened a library of organoselenium and organotellurium compounds for hydrogen peroxide scavenging activity, using macrophagic cell lines RAW264.7 and THP-1, as well as human mono- and poly-nuclear cells. These cells were stimulated to release H2O2, using phorbol 12-myristate 13-acetate, with and without organochalogens. Released H2O2 was then measured using a chemiluminescent assay over a period of 2 h. The screening identified an organoselenium compound which scavenged H2O2 more effectively than the vitamin E analog, Trolox. We also found that this organoselenium compound protected MC3T3 cells against H2O2-induced toxicity, whereas Trolox did not. The organoselenium compound exhibited no cytotoxicity to the cells and had no deleterious effects on cell proliferation, viability, or alkaline phosphatase activity. The rapidity of H2O2 scavenging and protection suggests that the mechanism of protection is due to the direct scavenging of extracellular H2O2. This compound is a promising modulators of inflammation and could potentially treat diseases involving high levels of oxidative stress.

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“…Ultimately, these enzymes are trafficked to sources of particular ROSs where they are most effective. In contrast, Trolox appears to need higher concentrations than used here thus likely to be a directly acting antioxidant against H2O2 and other ROSs [37]. …”

Section: Post-treatment With Antioxidantsmentioning

confidence: 77%

Neuroprotective Effect of Artocarpus Lakoocha Extract and Oxyresveratrol Against Hydrogen Peroxide-Induced Toxicity in Sh-Sy5y Cells

Hasriadi

Wongon

Lapphanichayakool

et al. 2017

Int J Pharm Pharm Sci

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Objective: Artocarpuslakoocha Roxb. Is a traditional medical plant native to Southeast Asia and used as a dried aqueous extract so-called puaghaad. Its role (and its major ingredient, oxyresveratrol) as an antioxidant neuroprotectant were explored.Methods: Differentiated SH-SY5Y neuroblastoma cells in 96-well plates were challenged with 200 µM H2O2 for 4h and either Trolox (100 µM), oxyresveratrol (5-100 µM), or puaghaad (1.2-25 µg/ml) applied 2h before H2O2 or for 20 h after H2O2Results: Continuous presence of both H washout. Cell viability, mitochondrial function, intracellular ROS, and lipid peroxidation were assessed.2O 2 and antioxidant reduced mitochondrial function by ~50% but only by 30% with antioxidant. Sustained 24 h H2O2 showed no recoveries with antioxidants. Cell viability was modestly restored when antioxidants accompanied H2O2 for 4 h and both washed for another 20 h, but little recovery of mitochondrial function even though antioxidants removed ROS and prevent lipid peroxidation. Antioxidants added for 20 h after H2O2 Conclusion:These results show that mitochondrial protection was illusive, yet both tested compounds, puaghaad and oxyresveratrol, improved cell viability and especially ROS levels and lipid peroxidation. The potency oxyresveratrol on theredox-sensitive expression of antioxidant enzymes and its pharmacokinetics suggests that oral puaghaad could provide effective protection in transient neurodegenerative disease. marginally improve mitochondria and modestly restore cell viability, but lipid peroxidation was completely reversed.

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Intracellular scavenging activity of Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid) in the fission yeast, Schizosaccharomyces pombe

Cited by 46 publications

References 25 publications

Differential Effects of MitoVitE, α-Tocopherol and Trolox on Oxidative Stress, Mitochondrial Function and Inflammatory Signalling Pathways in Endothelial Cells Cultured under Conditions Mimicking Sepsis

Differential Effects of MitoVitE, α-Tocopherol and Trolox on Oxidative Stress, Mitochondrial Function and Inflammatory Signalling Pathways in Endothelial Cells Cultured under Conditions Mimicking Sepsis

Selenium- and Tellurium-Based Antioxidants for Modulating Inflammation and Effects on Osteoblastic Activity

Neuroprotective Effect of Artocarpus Lakoocha Extract and Oxyresveratrol Against Hydrogen Peroxide-Induced Toxicity in Sh-Sy5y Cells

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