Intracellular fluorescence decay time of anilinonaphthalene sulfonate

Loeser, Ch.N.; Clark, Ellen

doi:10.1016/0014-4827(72)90018-3

Cited by 9 publications

(1 citation statement)

References 11 publications

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“…These measurements were carried out by comparing decay curves from an analog simulator with the fluorescence decay on an oscilloscope 45,46 , with a homemade digital averager [47][48][49] , and by using Ortec photon counting instrumentation [50][51][52] . Because of low signal to noise, the measurement times were very long especially for photon counting methods when multi-exponential analyses were attempted.…”

Section: Fluorescence Lifetime-resolved Imagingmentioning

confidence: 99%

What is behind all those lifetimes anyway, and where do we go from here?

et al. 2009

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Fluorescence lifetime-resolved imaging microscopy (FLIM) has made tremendous strides in the last two decades. Exciting applications are being presented weekly, an extensive diversity of instrumentation and commercial devices have appeared and have improved dramatically, sophisticated algorithms for analysis and interpretation are now available, and FLIM is being coupled to other imaging modalities, such as spectral dispersion and anisotropy. In other words, FLIM has matured considerably, and is approaching a point where it can be routinely applied by researchers who are not involved with the instrumentation and analysis side of things. The number of interested users in FLIM has almost certainly surpassed that of the audience that previously employed single channel fluorescence lifetime measurements (in a cuvette). The reason is, of course, the imaging capability of FLIM and its exciting possibilities for biological applications, especially FRET. In this lecture I will attempt to give an overview of where we have come from together with a personal judgment on where we stand, propose possible areas of growth that will be of importance for the future of FLIM, and discuss some specific challenges that remain, especially considering the unique nature of the FLIM measurement and the complex biological and medical systems that require innovative and novel solutions from the FLIM community.

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Section: Fluorescence Lifetime-resolved Imagingmentioning

confidence: 99%

What is behind all those lifetimes anyway, and where do we go from here?

et al. 2009

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Fluorescent Probing of Dynamic and Molecular Organization of Biological Membranes

Badley¹

1976

Modern Fluorescence Spectroscopy

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Fluorescence lifetime-resolved imaging

Chen

Clegg

2009

Photosynth Res

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This is a short account of fluorescence lifetime-resolved imaging, in order to acquaint readers who are not experts with the basic methods for measuring lifetime-resolved signals throughout an image. We present the early FLI (fluorescence lifetime imaging) history, review shortly the instrumentation and experimental design, discuss briefly the fundamentals of the measured fluorescence response, and introduce the basic measurement methodologies. We also emphasize the complex nature of the fluorescence response in FLI signals, and introduce certain analysis methods that are appropriate and informative for complex fluorescence decays. The advantages of model independent analyses are discussed and examples given.

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Intracellular fluorescence decay time of anilinonaphthalene sulfonate

Cited by 9 publications

References 11 publications

What is behind all those lifetimes anyway, and where do we go from here?

What is behind all those lifetimes anyway, and where do we go from here?

Fluorescent Probing of Dynamic and Molecular Organization of Biological Membranes

Fluorescence lifetime-resolved imaging

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