Fluorescence lifetime-resolved imaging

Chen, Yi‐Chun; Clegg, Robert M.

doi:10.1007/s11120-009-9458-7

Cited by 41 publications

(28 citation statements)

References 48 publications

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“…The decay from the acceptor, after energy transfer, should be longer than if the acceptor was directly excited due to the time delay from absorption by the donor, energy transfer between the donor/acceptor pair and the subsequent emission from the acceptor. This delay is manifested as a lengthening of the measured phase and such a delay will move the phasor point outside the universal circle (see for example [57,58]). Fig.…”

Section: Resultsmentioning

confidence: 99%

Applications of phasors to in vitro time-resolved fluorescence measurements

Štefl

James

Ross

et al. 2011

Analytical Biochemistry

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The phasor method of treating fluorescence lifetime data provides a facile and convenient approach to characterize lifetime heterogeneity and to detect the presence of excited state reactions, such as solvent relaxation and Förster Resonance Energy Transfer. The method utilizes a plot of M sin(Φ) versus M cos(Φ), where M is the modulation ratio and Φ is the phase angle taken from frequency domain fluorometry. A principle advantage of the phasor method is that it provides a model-less approach to time-resolved data, amenable to visual inspection. Although the phasor approach has been recently applied to Fluorescence Lifetime Imaging Microscopy it has not been extensively utilized for cuvette studies. In the present study we explore the applications of the method to in vitro samples. The phasors of binary and ternary mixtures of fluorescent dyes demonstrates the utility of the method for investigating complex mixtures. Data from excited state reactions, such as dipolar relaxation in membrane and protein systems and also energy transfer from the tryptophan residue to the chromophore in EGFP, are also presented.

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Section: Resultsmentioning

confidence: 99%

Applications of phasors to in vitro time-resolved fluorescence measurements

Štefl

James

Ross

et al. 2011

Analytical Biochemistry

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“…FReI may thus provide a measure of the conformational space accessible to IDPs in cells, the dynamics of in-cell conformational changes and how these properties vary between organelles in the same cell, and from cell to cell. Such new and improved technologies along with continuing advancements in fluorescence microscopy methods, including both single molecule fluorescence microscopy and ensemble methods such as fluorescence lifetime imaging, 876 are likely to increase the utility of fluorescence microscopy for studying IDPs in cells.…”

Section: Theoretical and Experimental Methods To Study Idpsmentioning

confidence: 99%

Physicochemical Properties of Cells and Their Effects on Intrinsically Disordered Proteins (IDPs)

Binolfi²,

et al. 2014

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“…The observed decrease in phosphorescence lifetime makes this class of compounds a good candidate for applications in phosphorescence lifetime imaging microscopy, 27 in addition to its use as steady-state ratiometric O 2 sensor probes.…”

Section: Acs Medicinal Chemistry Lettersmentioning

confidence: 99%

Platinum(II) Ring-Fused Chlorins as Near-Infrared Emitting Oxygen Sensors and Photodynamic Agents

Pereira

Laranjo

Casalta-Lopes

et al. 2017

ACS Med. Chem. Lett.

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Novel near-infrared luminescent compounds based on platinum(II) 4,5,6,7-tetrahydropyrazolo[1,5-a]-pyridine-fused chlorins are described. These compounds have high photostability and display light emission, in particular simultaneous fluorescence and phosphorescence emission in solution at room temperature, in the biologically relevant 700−850 nm red and near-infrared (NIR) spectral region, making them excellent materials for biological imaging. The simultaneous presence of fluorescence and phosphorescence emission at room temperature, with the phosphorescence strongly quenched by oxygen whereas fluorescence remains unaffected, allows these compounds to be used as ratiometric oxygen sensors in chemical and biological media. Both steady-state (fluorescence vs phosphorescence intensities) and dynamic (dependence of phosphorescence lifetimes upon oxygen concentration) luminescence approaches can be used. Photocytotoxicity studies against human melanocytic melanoma cells (A375) indicate that these compounds display potential as photosensitizers in photodynamic therapy.

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Fluorescence lifetime-resolved imaging

Cited by 41 publications

References 48 publications

Applications of phasors to in vitro time-resolved fluorescence measurements

Applications of phasors to in vitro time-resolved fluorescence measurements

Physicochemical Properties of Cells and Their Effects on Intrinsically Disordered Proteins (IDPs)

Platinum(II) Ring-Fused Chlorins as Near-Infrared Emitting Oxygen Sensors and Photodynamic Agents

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