Modern Fluorescence Spectroscopy 1976
DOI: 10.1007/978-1-4684-2586-4_3
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Fluorescent Probing of Dynamic and Molecular Organization of Biological Membranes

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Cited by 33 publications
(14 citation statements)
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“…order parameter) on cholesterol but also its interaction strengths with phospholipid constituents of the membrane (12,15,16). While 1 H and 13 C labeled cholesterols provide probes with structure most closely resembling that of cholesterol, it is difficult to incorporate large amounts of these labeled sterols into biological membranes and the time-scale of NMR measurements precludes resolution of cholesterol dynamics on the biological time-scale (17). Another technique, electron spin resonance (EPR) involves the exploitation of the paramagnetism of certain molecules with unpaired spins, usually 14 N (15).…”
Section: Historical Perspectivementioning
confidence: 99%
See 1 more Smart Citation
“…order parameter) on cholesterol but also its interaction strengths with phospholipid constituents of the membrane (12,15,16). While 1 H and 13 C labeled cholesterols provide probes with structure most closely resembling that of cholesterol, it is difficult to incorporate large amounts of these labeled sterols into biological membranes and the time-scale of NMR measurements precludes resolution of cholesterol dynamics on the biological time-scale (17). Another technique, electron spin resonance (EPR) involves the exploitation of the paramagnetism of certain molecules with unpaired spins, usually 14 N (15).…”
Section: Historical Perspectivementioning
confidence: 99%
“…1B-F) in living cultured cells or in vivo. Fluorescent sterols, like other fluorescent probes, provide ease of handling with very high level sensitivity of detection, relatively short detection times, and multiplexing of several probes (17). These characteristics of fluorescence detection have made it very beneficial in utilizing small quantities of fluorescent sterols not only for use with traditional spectrofluorometers but also in newly developed systems.…”
Section: Advent Of Fluorescent Sterolsmentioning
confidence: 99%
“…As can be seen, the FP of SConA-F is significantly higher than that of ConA-F (0.258 versus 0.188). This may be explained by assuming two competing causes of homotransfer, which both, by and large, lower FP (Badley, 1976): the first, between fluorescein molecules settling on different ConA molecule, the second, between fluorescein molecules settling on the same ConA molecule. The first cause might be dominant in the case of capping, which increase the proximity among fluorescein molecules, such as in the case of ConA-F treated cells in comparison with SConA-F treated cells.…”
Section: Use Of Succinylated Cona For No-capping Situationmentioning
confidence: 99%
“…Working towards this goal several laboratories have performed fluorescence measurements on globally oriented samples (Yguerabide and Stryer, 1971;Badley et al, 1971Badley et al, , 1973Badley et al, , 1976Frehland et al, 1982;Kooyman et al, 1981Kooyman et al, , 1983Vos et al, 1983). The data are acquired in anisotropic fashion, that is the orientation of the lipid bilayer in the laboratory frame is known.…”
Section: Introductionmentioning
confidence: 99%