Intracellular and extracellular microRNA: An update on localization and biological role

Makarova, J. A.; Shkurnikov, M. Yu.; Wicklein, Daniel; Lange, Tobias; Samatov, Timur R.; Turchinovich, Andrey; Tonevitsky, Alexander G.

doi:10.1016/j.proghi.2016.06.001

Cited by 201 publications

(147 citation statements)

References 184 publications

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“…This fact supports the idea that EVs should alter the host cell. MicroRNAs have been shown to have a role in cancer, cell reprogramming, hypertension regulation and other chronic diseases (reviewed by Tao et al, 2016;Stepicheva and Song, 2016;Makarova et al, 2016), and could be essential in host-parasite cell interactions.…”

Section: Exosome and Microvesicles Content Can Alter Neighbouring Cellsmentioning

confidence: 99%

Microvesicles released from Giardia intestinalis disturb host-pathogen response in vitro

Evans-Osses

Mojoli

Monguió‐Tortajada

et al. 2017

European Journal of Cell Biology

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a b s t r a c tGiardia intestinalis (G.I), is an anaerobic protozoan and the aetiological agent of giardiasis, a diarrhoea present worldwide and associated with poverty. G.I has a simple life cycle alternating between cyst and trophozoite. Cysts are transmitted orally to the stomach and transform to trophozoites in the intestine by a multifactorial process. Recently, microvesicles (MVs) have been found to be released from a wide range of eukaryotic cells. We have observed a release of MVs during the life cycle of G.I., identifying MVs from active trophozoites and from trophozoites differentiating to the cyst form. The aim of the current work was to investigate the role of MVs from G.I in the pathogenesis of giardiasis. MVs from log phase were able to increase the attachment of G. intestinalis trophozoites to Caco-2 cells. Moreover, MVs from G. intestinalis could be captured by human immature dendritic cells, resulting in increased activation and allostimulation of human dendritic cells. Lipid rafts participate in the MV biogenesis and in the attachment to Caco-2 cells. Nevertheless, proteomic analysis from two types of MVs has shown slight differences at the protein levels. An understanding of biogenesis and content of MVs derived from trophozoites might have important implications in the pathogenesis of the disease.

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Section: Exosome and Microvesicles Content Can Alter Neighbouring Cellsmentioning

confidence: 99%

Microvesicles released from Giardia intestinalis disturb host-pathogen response in vitro

Evans-Osses

Mojoli

Monguió‐Tortajada

et al. 2017

European Journal of Cell Biology

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“…Previous studies have revealed that miR-200b are down-regulated in AIPC cells and that this phenomenon contributes to androgenindependent growth [3,4]. Functional characterization of miRNAs depends strongly on identification of their specific mRNA binding partners because miRNAs can prevent protein expression by binding either the 3′UTR of the mRNA target through imperfect complementarity or via multiple sites by inhibition of the mRNA interaction with ribosomal complex and the translational machinery [5]. This kind of imperfect complementarity with target ensures that miRNAs have multiple intracellular targets that lead to amplification of the biological effects [6,7].…”

Section: Introductionmentioning

confidence: 99%

Label free quantitative proteomics reveals the role of miR-200b in androgen-independent prostate cancer cells

Gou

et al. 2018

Clin Proteom

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Background: Our previous studies indicated that miR-200b inhibits the growth of androgen-independent prostate cancer (AIPC) cells. In this study, we employed quantitative proteomics techniques to unravel the role of miR-200b in AIPC. Results: Thirteen proteins were up-regulated in miR-200b mimics/mimics NC (negative miRNA control) and 14 proteins were down-regulated; 67 proteins were up-regulated in miR-200b inhibitor/inhibitor NC and 98 proteins were down-regulated. There were seven proteins which were both down-regulated by miR-200b mimics and up-regulated by miR-200b inhibitor, TM4SF1, YAP1, PPP1R2, MARCKS, RTN4, GLIPR2 and SUCLG1. Among these, TM4SF1, YAP1, PPP1R2, MARCKS, RTN4 were predicted as target genes of miR-200b by miRBase, while GLIPR2 and SUCLG1 were not. Methods Conclusion:This work identified several target genes of miR-200b by label free proteomics method, i.e., TM4SF1, YAP1, PPP1R2, MARCKS, RTN4, GLIPR2, and SUCLG1. The signaling pathways regulated by these proteins such as Hippo signaling may contribute to the phenotype resulting from miR-200b.

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“…However, recent findings suggest miRNA stabilization can be different as well. Makarova et al, 2016, revealed that in HeLa cells, the amount of miRNA is approximately 13 times higher than the amount of AGO proteins (~200,000 and 15,000 molecules per cell, respectively) (20). Moreover, two recent studies have reported the discovery of more than a thousand new human miRNAs (19,33).…”

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confidence: 99%

“…Many of these miRNA structures strongly resembled to anti-tenascin C aptamers (37) implying that miRNAs may directly modulate protein activity (36), but also it may imply that miRNAs may have the ability to bind to different proteins and become stabilized or be sorted for EV loading. Exosomes studies have shown that among several pathways of exosome biogenesis, the ceramide-dependent mechanism is a way contributing to circulating miRNA release since export of miRNA outside the cell was impaired upon inhibition of neutral sphingomyelinase 2 (nSMase2) an enzyme mediating ceramide biosynthesis (20,(38)(39)(40). Another way of miRNAs secretion mediated by ESCRT machinery of exosome formation seems to be more controversial since in HEK293 cells, after transient knock-down of the ESCRTassociated protein ALIX, which regulates ESCRT-dependent intraluminal vesicle (ILV) formation (41) the levels of miRNA in conditioned media remained unaltered (42).…”

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confidence: 99%

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Future directions of extracellular vesicle-associated miRNAs in metastasis

López

Granados-López

2017

Ann. Transl. Med.

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with the near and long distance microenvironment allows metastasis. These studies will surely conduce to additional patient studies to prove the relevance of EV miRNAs in metastasis in vivo. It remains to be elucidated how the tumoral cell sorts the miRNAs for secretion to send a message, and to well recognize the type of EV performing this message delivering. It will be very useful to identify whether miRNAs are delivered with post-transcriptional modifications since this is an important feature for miRNAs activity and stability.

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Intracellular and extracellular microRNA: An update on localization and biological role

Cited by 201 publications

References 184 publications

Microvesicles released from Giardia intestinalis disturb host-pathogen response in vitro

Microvesicles released from Giardia intestinalis disturb host-pathogen response in vitro

Label free quantitative proteomics reveals the role of miR-200b in androgen-independent prostate cancer cells

Future directions of extracellular vesicle-associated miRNAs in metastasis

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