Label free quantitative proteomics reveals the role of miR-200b in androgen-independent prostate cancer cells

Abstract

Background: Our previous studies indicated that miR-200b inhibits the growth of androgen-independent prostate cancer (AIPC) cells. In this study, we employed quantitative proteomics techniques to unravel the role of miR-200b in AIPC. Results: Thirteen proteins were up-regulated in miR-200b mimics/mimics NC (negative miRNA control) and 14 proteins were down-regulated; 67 proteins were up-regulated in miR-200b inhibitor/inhibitor NC and 98 proteins were down-regulated. There were seven proteins which were both down-regulated by miR-200b mimics and up-regulated by miR-200b inhibitor, TM4SF1, YAP1, PPP1R2, MARCKS, RTN4, GLIPR2 and SUCLG1. Among these, TM4SF1, YAP1, PPP1R2, MARCKS, RTN4 were predicted as target genes of miR-200b by miRBase, while GLIPR2 and SUCLG1 were not. Methods Conclusion:This work identified several target genes of miR-200b by label free proteomics method, i.e., TM4SF1, YAP1, PPP1R2, MARCKS, RTN4, GLIPR2, and SUCLG1. The signaling pathways regulated by these proteins such as Hippo signaling may contribute to the phenotype resulting from miR-200b.

Applications of Prostate Cancer Proteomics: A Review

“…A liquid chromatography analysis of the protein regulated by miR-200b confirmed the findings presented by Asuthkar et al (2016). He et al (2018) used prostate cancer cell lines to demonstrate the involvement of transmembrane 4 L6 family member 1 (TM4SF1), yes-associated protein 1 (YAP1), Protein phosphatase inhibitor 2 (PPP1R2), Myristoylated alanine-rich C-kinase substrate (MARCKS), and Reticulon 4 (RTN4) inhibitors in reducing the progression of prostate cancer. The inhibitors analysed targeted the Wnt pathway.…”

Cell fate regulation by reticulon‐4 in human prostate cancers

“…PC‐3, DU145, and LNCaP are prostate cancer cell lines that widely used in prostate cancer research. Cheng et al () reported that RTN4 was involved in the biological processes regulated by the androgen receptor, while He et al () label‐free quantitative proteomics study proved that RTN4 targeted by miR‐200b also played a role in androgen‐independent prostate cancer cells. Hence, we did not consider the androgen‐dependent or independent status of prostate cancer cells.…”

Advancements in Oncoproteomics Technologies: Treading toward Translation into Clinical Practice

“…Label-free quantitation is easy to use, yields highly reproducible results, and is reliable [ 243 , 244 , 245 ]. It is cost-effective (avoids expensive chemical and metabolic tags) and allows the profiling of a number of large samples with the flexibility of multiple comparisons [ 246 , 247 ]. It eliminates the chance of variability that chemical labeling/tagging introduces and significantly reduces the sample preparation time by eliminating numerous steps [ 248 ].…”