Intra-articular injections of ketamine and 25% dextrose improve clinical and pathological outcomes in the monosodium iodoacetate model of osteoarthritis

Shetty, Yashashri C; Patil, Amol E.; Jalgaonkar, Sharmila; Rege, Nirmala N; Salgaonkar, Sweta; Teltumbde, Prachi A.; Kshirsagar, Sarang; Koli, Paresh G; Brahma, Smita

doi:10.1515/jbcpp-2016-0135

Cited by 8 publications

(5 citation statements)

References 18 publications

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“…In addition, the fluorescence intensity of the TKCP was also investigated. The mice were randomly sorted into three groups (n = 3): 0.05 mg MIA + inhibitor group, IA injection of 0.05 mg MIA concomitant with 5 mM NAC; 0.05 mg MIA group, IA injection of 0.05 mg MIA; 0.1 mg MIA group, IA injection of 0.1 mg MIA [ 44 , 45 ]. Finally, the mice were sacrificed and the macroscopic evaluations of joints were performed.…”

Section: Methodsmentioning

confidence: 99%

Reactive oxygen species (ROS)-responsive nanoprobe for bioimaging and targeting therapy of osteoarthritis

et al. 2021

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Stimulus-responsive therapy that allows precise imaging-guided therapy is limited for osteoarthritis (OA) therapy due to the selection of proper physiological markers as stimulus. Based on that the over-production of Reactive Oxygen Species (ROS) is associated with the progression in OA, we selected ROS as markers and designed a cartilage targeting and ROS-responsive theranostic nanoprobe that can be used for effective bioimaging and therapy of OA. This nanoprobe was fabricated by using PEG micelles modified with ROS-sensitive thioketal linkers (TK) and cartilage-targeting peptide, termed TKCP, which was then encapsulated with Dexamethasone (DEX) to form TKCP@DEX nanoparticles. Results showed that the nanoprobe can smartly “turn on” in response to excessive ROS and “turn off” in the normal joint. By applying different doses of ROS inducer and ROS inhibitor, this nanoprobe can emit ROS-dependent fluorescence according to the degree of OA severity, helpful to precise disease classification in clinic. Specifically targeting cartilage, TKCP@DEX could effectively respond to ROS and sustained release DEX to remarkably reduce cartilage damage in the OA joints. This smart, sensitive and endogenously activated ROS-responsive nanoprobe is promising for OA theranostics. Graphical Abstract

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Section: Methodsmentioning

confidence: 99%

Reactive oxygen species (ROS)-responsive nanoprobe for bioimaging and targeting therapy of osteoarthritis

et al. 2021

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“…In 2017, Yashashri C. Shetty and his colleagues did research work on chemicalinduced models of rats. Their results confirmed that triamcinolone reduces the histopathological severity of disease as compared to the disease control group [21] . In vitro study of E. Frank and fellows affirmed that triamcinolone has some chondroprotective effects on injured and inflamed cartilage via inhibiting sulfate incorporation and glycosaminoglycan loss [22] .…”

Section: Discussionmentioning

confidence: 55%

Evaluation of Chondroprotective Efficacy of Triamcinolone in Osteoarthritis Induced Rat Model

Ishaq¹,

Mehdi

Bajwa

et al. 2021

JUMDC

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ABSTRACT BACKGROUND AND OBJECTIVE: Osteoarthritis is one of the most common joint diseases afflicting human, characterized by progressive degeneration of articular cartilage in which chondrocytes fails to adequately repair. Objective of this study is to evaluate the chondroprotection offered by triamcinolone in osteoarthritis induced rat model METHODOLOGY: This Laboratory based experimental study was conducted in Department of Pharmacology, Army Medical College, Rawalpindi, in collaboration with National Institute of health, Islamabad from April-June2019. Osteoarthritis was induced by surgical removal of medial meniscus and anterior cruciate ligament resection in right knee joint of Sixteen (16) anesthetized rats of Sprague Dawley breed. They were divided in two (02) groups with eight (08) rats in each group. Group I was disease control in which 0.2 ml Intra articular saline was administered for three weeks. While group II was treatment group that was treated by 70 µl intra articular triamcinolone once weekly for three weeks. After that gait pattern of rats was scored. Animals were euthanized with over dosage of inhaled chloroform and sample of proximal tibia was taken for histopathological analysis.RESULTS: Mean gait score of control group and treatment group was 3.25±.707 and 2.25±.463 with a p value of .028 that is statistically significant. While mean histopathological modified Mankin score of control and treatment group was 11.5±1.195 and 8.5±1.195 respectively with a significant P-value of <0.01. CONCLUSION: Intra articular administration of triamcinolone in Osteoarthritis induced rats resulted in improvement in gait pattern and histopathology. Keywords: Chondroprotective efficacy, Osteoarthritis, Triamcinolone.

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“…21 In 2017, Yashashri C. Shetty and his colleagues did research work on chemically induced models of rats and concluded that TRI lessens the histopathological severity of disease (p<0.01) as compared to disease control group. 22 In vitro study of E. Frank also affirmed that TRI has some chondroprotective effects ( p value <0.05) on injured and inflamed cartilage inhibiting sulphate incorporation and glycosaminoglycan loss. 23 When gait and Modified Mankin score of HA group was statistically compared with TRI group, it was analysed that HA is more efficacious than TRI.…”

Section: Discussionmentioning

confidence: 88%

“…These results are according to the research work of Yashashri C. Shetty who found that HA has better chondroprotective effects than TRI in chemically induced murine model of OA (p<0.001). 22 Likewise, human study by Soad A Elsawy and his colleagues depicted superior chondroprotection offered by HA as compared to TRI (p=0.01). 24 Similarly, metaanalysis by Egemen Ayhan and colleagues also verified that HA has better chondroprotective effects as compared to TRI.…”

Section: Discussionmentioning

confidence: 96%

Comparative Chondroprotective Effects of Intra-Articular Hyaluronic Acid and Triamcinolone in Murine Model of Osteoarthritis

Ishaq

Gul

et al. 2022

J Ayub Med Coll Abbottabad

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Osteoarthritis is a heterogeneous disease of joints that affects mainly older population. Definitive cure of osteoarthritis is still undiscovered. This study was designed to evaluate and compare the chondroprotective effects of hyaluronic acid and triamcinolone in murine model of osteoarthritis. Methods: This Laboratory based experimental study was carried out in Pharmacology Department, Army Medical College Rawalpindi, from April-June 2019. Osteoarthritis was induced by medial menisectomy and anterior cruciate ligament resectioning of knee joints of twenty-four rats which were then divided into three groups with eight rats in each. Group I, II and III were named control, hyaluronic acid and triamcinolone groups respectively, which were given intra-articular injections of these drugs once weekly for four consecutive weeks and then gait pattern was scored. Animals were sacrificed thereafter and samples were collected for histopathological analysis. Results: Comparison of gait score of control, hyaluronic acid and triamcinolone groups exhibited a p value of <0.01 while intergroup comparison between group I and II, group I and III and group II and III depicted p value of <0.001, 0.016 and 0.003 respectively. Likewise, collective histopathological analysis of the three groups showed p value of <0.01 while intergroup comparison of group I and II, group I and III and group II and III showed p value of <0.001 for all. Conclusion: Comparison of control group with treatment groups proved chondroprotective effects of hyaluronic acid and triamcinolone. Additionally, hyaluronic acid proved to provide better chondroprotection as compared to triamcinolone.

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Intra-articular injections of ketamine and 25% dextrose improve clinical and pathological outcomes in the monosodium iodoacetate model of osteoarthritis

Abstract: The NMDA antagonist effect of ketamine and the proliferative effect of 25% dextrose showed a reduction in pain and disease activity in the OA model.

Cited by 8 publications

References 18 publications

Reactive oxygen species (ROS)-responsive nanoprobe for bioimaging and targeting therapy of osteoarthritis

Reactive oxygen species (ROS)-responsive nanoprobe for bioimaging and targeting therapy of osteoarthritis

Evaluation of Chondroprotective Efficacy of Triamcinolone in Osteoarthritis Induced Rat Model

Comparative Chondroprotective Effects of Intra-Articular Hyaluronic Acid and Triamcinolone in Murine Model of Osteoarthritis

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