Intra- and inter-individual comparison of Porphyromonas gingivalis genotypes

Saarela, Maria

doi:10.1016/0928-8244(93)90076-g

Cited by 16 publications

(20 citation statements)

References 13 publications

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“…III (VMGA III) medium, and cultured. A. actinomycetemcomitans and P. gingivalis were identified as previously described (18, 19). Briefly, for A. actinomycetemcomitans , 100-µl aliquots of undiluted and 10 −2 dilutions of samples were inoculated on Trypic soy bacitracin vancomycin (TSBV)-agar plates and incubated in 5% CO 2 in air at 36°C for three days.…”

Section: Methodsmentioning

confidence: 99%

Tracking of plasma antibodies againstAggregatibacter actinomycetemcomitansandPorphyromonas gingivalisduring 15 years

Lakio

Antinheimo

Paju

et al. 2009

Journal of Oral Microbiology

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Background Plasma antibody measurements of antibody levels to periodontal pathogens may be used to support diagnosis, disease activity, classification, and prognosis of periodontitis.Objective The aim of this study was to investigate the long-term stability of plasma antibody levels against Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis.Design Plasma immunoglobulin G (IgG) antibody levels against the pathogens were analyzed annually during 15 years from 21 voluntary subjects, whose periodontal status was not known at the point of selection. The total number of plasma samples was 315. In connection of the last sampling, the clinical and radiographic periodontal status was examined. Pooled bacterial samples from periodontal pockets, as well as salivary samples were collected for A. actinomycetemcomitans and P. gingivalis detection, and antibody determinations, respectively. According to the clinical status, six subjects had periodontitis, whereas 15 did not.Results Plasma IgG-class antibody levels to periodontal pathogens remained extremely stable during the 15-year period and no significant (p>0.05) intra-individual variations were observed. Retrospectively, the average plasma IgG antibody levels against A. actinomycetemcomitans and P. gingivalis were 1.6–2.3 (p<0.05) and 1.4–1.7 (p<0.05) fold higher in the subjects with periodontitis than those without, respectively, during the whole 15-year tracking. As expected, at the time of the periodontal examination the plasma and salivary IgG antibody levels were associated both with periodontitis and bacterium-positivity.ConclusionsPlasma IgG levels against A. actinomycetemcomitans and P. gingivalis are extremely stable during 15 years both in subjects with and without periodontitis.

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Section: Methodsmentioning

confidence: 99%

Tracking of plasma antibodies againstAggregatibacter actinomycetemcomitansandPorphyromonas gingivalisduring 15 years

Lakio

Antinheimo

Paju

et al. 2009

Journal of Oral Microbiology

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“…Caucasian subjects usually harbour one P . gingivalis clonal type (Loos et al 1992, Saarela et al 1993a, 1996, van Steenbergen et al 1993) but multiple clones per subject have also been reported (Enersen et al 2006). Recently, van Winkelhoff et al (2007) reported on multiple clonal types in 34.5% of the subjects in an Indonesian population with varying degrees of periodontal disease.…”

mentioning

confidence: 99%

Clonal stability of Porphyromonas gingivalis in untreated periodontitis

Winkelhoff¹,

Rijnsburger²,

Velden

2008

J Clinic Periodontology

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“…Socransky and Martin [34] and Saarela et al [35] used ribotyping to distinguish clonal types of P. gingivalis. In this method, chromosomal DNA is digested with restriction endonucleases, subjected to electrophoresis and Southern blotting, after which it is hybridised with ribosomal DNA.…”

Section: Present Statusmentioning

confidence: 99%

“…In this method, chromosomal DNA is digested with restriction endonucleases, subjected to electrophoresis and Southern blotting, after which it is hybridised with ribosomal DNA. Isolates from six [34] and nine [35] different subjects, respectively, were found to be of different types. An advantage of this method compared with whole genomic DNA fingerprinting is the lower number of bands obtained, which facilitates comparison of lanes.…”

Section: Present Statusmentioning

confidence: 99%

Classification and typing methods of black-pigmented Gram-negative anaerobes

VANSTEENBERGEN¹,

VANWINKELHOFF²,

DEGRAAFF³

1993

FEMS Immunology and Medical Microbiology

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Until recently, black-pigmented Gram-negative anaerobes were classified as 'black-pigmented Bacteroides'. At present, 11 distinct species are recognized in this group. Because of major differences with Bacteroides fragilis, the type species of the genus Bacteroides, new genera have been proposed: Porphyromonas for three asaccharolytic species, and Prevotella for the saccharolytic species. Typing methods have been developed for some species of black-pigmented Gram-negative anaerobes. These include biotyping and serotyping, but relatively few types can be distinguished with these methods. Recently, DNA restriction endonuclease analysis has been used for typing of P. gingivalis, Pr. intermedia and P. endodontalis strains. Great heterogeneity was observed within all three species. This typing method can be useful for epidemiological studies.

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Intra- and inter-individual comparison of Porphyromonas gingivalis genotypes

Cited by 16 publications

References 13 publications

Tracking of plasma antibodies againstAggregatibacter actinomycetemcomitansandPorphyromonas gingivalisduring 15 years

Tracking of plasma antibodies againstAggregatibacter actinomycetemcomitansandPorphyromonas gingivalisduring 15 years

Clonal stability of Porphyromonas gingivalis in untreated periodontitis

Classification and typing methods of black-pigmented Gram-negative anaerobes

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