Clonal stability of <i>Porphyromonas gingivalis</i> in untreated periodontitis

Winkelhoff, A.J. van; Rijnsburger, Martine; Velden, U. van der

doi:10.1111/j.1600-051x.2008.01285.x

Cited by 19 publications

(18 citation statements)

References 26 publications

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“…Although the numbers of patients colonized before as well as after the intervention were too small to make final conclusions, this finding suggests that patients with periodontal disease are infected with a P. gingivalis clone over longer periods of time. The finding is in line with a recent report by van Winkelhoff et al (2008), who demonstrated by amplified fragment length polymorphism analysis the longitudinal within-host clonal stability of P. gingivalis in a human population lacking periodontal care over a period of 8 years, however without therapeutic intervention. Further investigations will be necessary to understand whether the observed recolonization of the periodontal pockets by P. gingivalis is the result of bacterial persistence in the periodontal pockets despite of therapy, or whether intrafamiliar transmission is causative (Asikainen et al, 1996;Saarela et al, 1993;van Winkelhoff and Boutaga, 2005).…”

Section: Article In Presssupporting

confidence: 92%

Microbial changes in periodontitis successfully treated by mechanical plaque removal and systemic amoxicillin and metronidazole

Valenza

Veihelmann

Peplies

et al. 2009

International Journal of Medical Microbiology

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Section: Article In Presssupporting

confidence: 92%

Microbial changes in periodontitis successfully treated by mechanical plaque removal and systemic amoxicillin and metronidazole

Valenza

Veihelmann

Peplies

et al. 2009

International Journal of Medical Microbiology

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“…However, there was a time lapse between periodontal examination and sample collection, and it was possible that P. gingivalis clones were replaced during that interval; still, the chances for such clonal change may be low because it is reported that P. gingivalis showed high clonal stability (Valenza et al, 2009; Van Winkelhoff, Rijnsburger & Van Der Velden, 2008). In addition, we would like to note that most similar studies have the same problems which are inherent to this type of clinical research, because generally the treatment for chronic periodontitis takes a long time.…”

Section: Discussionmentioning

confidence: 99%

Distribution ofPorphyromonas gingivalis fimAandmfa1fimbrial genotypes in subgingival plaques

Nagano

Hasegawa

Iijima

et al. 2018

PeerJ

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BackgroundStrains of periodontal disease-associated bacterium Porphyromonas gingivalis have different pathogenicity, which can be attributed to clonal genetic diversity. P. gingivalis typically expresses two types of fimbriae, FimA and Mfa1, which comprise six (I, Ib, II, III, IV, and V) and two (mfa53 and mfa70) genotypes, respectively. This study was conducted to investigate the distribution of the two fimbrial genotypes of P. gingivalis in clinical specimens.MethodsSubgingival plaques were collected from 100 participants during periodontal maintenance therapy and examined for P. gingivalis fimbrial genotypes by direct polymerase chain reaction and/or DNA sequencing. We also analyzed the relationship between fimbrial genotypes and clinical parameters of periodontitis recorded at the first medical examination.ResultsBoth fimbrial types could be detected in 63 out of 100 samples; among them, fimA genotype II was found in 33 samples (52.4%), in which the mfa70 genotype was 1.75 times more prevalent than mfa53. The total detection rate of fimA genotypes I and Ib was 38.1%; in these samples, the two mfa1 genotypes were observed at a comparable frequency. In two samples positive for fimA III (3.2%), only mfa53 was detected, whereas in four samples positive for fimA IV (6.3%), the two mfa1 genotypes were equally represented, and none of fimA V-positive samples defined the mfa1 genotype. No associations were found between clinical parameters and fimbrial subtype combinations.DiscussionBoth P. gingivalis fimbrial types were detected at various ratios in subgingival plaques, and a tendency for fimA and mfa1 genotype combinations was observed. However, there was no association between P. gingivalis fimbrial genotypes and periodontitis severity.

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“…Fourteen sibling pairs, one set of three siblings (family number 9) as well as one set of four siblings (family number 8) participated in our study. From genotyping studies it is known that both vertical (from mother to child) and horizontal (among siblings and between spouses) transmission of oral microorganisms can occur, e.g ., identical P. gingivalis genotypes among siblings were found in 26% of the sibships [29], while in two of the three families from which strains from siblings were available, a common Streptococcus mutans ribotype occurred in all family members [30]. Recently even the transmission of S. mutans between unrelated children at public schools was reported [31].…”

Section: Discussionmentioning

confidence: 99%

Exploring the oral microbiota of children at various developmental stages of their dentition in the relation to their oral health

Crielaard

Zaura

Schuller³

et al. 2011

BMC Med Genomics

261

242

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BackgroundAn understanding of the relation of commensal microbiota to health is essential in preventing disease. Here we studied the oral microbial composition of children (N = 74, aged 3 - 18 years) in natural transition from their deciduous to a permanent dentition and related the microbial profiles to their oral health status. The microbial composition of saliva was assessed by barcoded pyrosequencing of the V5-V6 hypervariable regions of the 16 S rRNA, as well as by using phylogenetic microarrays.ResultsPyrosequencing reads (126174 reads, 1045 unique sequences) represented 8 phyla and 113 higher taxa in saliva samples. Four phyla - Firmicutes, Bacteriodetes, Proteobacteria and Actinobacteria - predominated in all groups. The deciduous dentition harboured a higher proportion of Proteobacteria (Gammaproteobacteria, Moraxellaceae) than Bacteroidetes, while in all other groups Bacteroidetes were at least as abundant as Proteobacteria. Bacteroidetes (mainly genus Prevotella), Veillonellaceae family, Spirochaetes and candidate division TM7 increased with increasing age, reflecting maturation of the microbiome driven by biological changes with age.Microarray analysis enabled further analysis of the individual salivary microbiota. Of 350 microarray probes, 156 gave a positive signal with, on average, 77 (range 48-93) probes per individual sample.A caries-free oral status significantly associated with the higher signal of the probes targeting Porphyromonas catoniae and Neisseria flavescens.ConclusionsThe potential role of P. catoniae and N. flavescens as oral health markers should be assessed in large-scale clinical studies. The combination of both, open-ended and targeted molecular approaches provides us with information that will increase our understanding of the interplay between the human host and its microbiome.

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Clonal stability of Porphyromonas gingivalis in untreated periodontitis

Abstract: The clonal stability of P. gingivalis under natural conditions is high. Complete different genotype distribution was found in only 27% of the subjects. Transmission of P. gingivalis occurred frequently among siblings but not among spouses.

Cited by 19 publications

References 26 publications

Microbial changes in periodontitis successfully treated by mechanical plaque removal and systemic amoxicillin and metronidazole

Microbial changes in periodontitis successfully treated by mechanical plaque removal and systemic amoxicillin and metronidazole

Distribution ofPorphyromonas gingivalis fimAandmfa1fimbrial genotypes in subgingival plaques

Exploring the oral microbiota of children at various developmental stages of their dentition in the relation to their oral health

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