Intestinal morphology and activity of digestive enzymes in broilers fed crude propolis

Duarte, Cristiane Regina do Amaral; EyngC.,; MurakamiA., E.; Santos, T. C.

doi:10.4141/cjas2013-059

Cited by 10 publications

(14 citation statements)

References 48 publications

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“…Results indicate that inclusion levels of RP and EEP could be used in chicken diets without any adverse effect on performance from 1 to 21 days of age, as previously observed by Duarte et al. () and Eyng et al. ().…”

Section: Discussionsupporting

confidence: 86%

“…The performance was determined at the end of experiment to exclude any negative effects of RP or EEP inclusion on feed ingestion or growth. Results indicate that inclusion levels of RP and EEP could be used in chicken diets without any adverse effect on performance from 1 to 21 days of age, as previously observed by Duarte et al (2014) and Eyng et al (2014). Although no differences in body weight gain were found among the treatments with both propolis products, the body weight gain in chickens fed RP was higher than those showed by chickens fed EEP, which cannot be explained only by the diets, considering the composition of basal diet was the same for the both experiments.…”

Section: Discussionsupporting

confidence: 80%

“…Morphophysiological changes in the gastrointestinal tract of chickens fed raw propolis were reported by Duarte et al (2014) and it is possible that this product could also modulate the intestinal microbiota, with lower cost compared to EEP. It is important to evaluate the effects of both propolis-derived products, due to differences in the concentration of active compounds to obtain a dose adjustment in order to promote a stimulation of such beneficial bacteria and protection against pathogenic micro-organisms.…”

Section: Introductionmentioning

confidence: 94%

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Caecal microbiota of chickens fed diets containing propolis

Eyng

Murakami

Pedroso³

et al. 2016

Animal Physiology Nutrition

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The present study aimed to evaluate the effect of different levels of ethanolic extract of propolis (EEP) and raw propolis (RP) on broiler performance and on selected bacterial groups in caecal microbiota using fluorescent in situ hybridization (FISH) measured by fluorescent activated cell sorting. Two experiments were conducted with 120 male chicks from 1 to 21 days of age for each, raised in cages and distributed in a completely randomized experimental design; there were five replicates with four birds per experimental unit and six treatments for each experiment (trial 1 - EEP - 0, 1000, 2000, 3000, 4000 and 5000 ppm and trial 2 - RP - 0, 100, 200, 300, 400 and 500 ppm). Fluorescent probes were used against the bacterial groups in caecal samples collected at 21 days of age. The data were subjected to one-way anova followed by Tukey's and regression analyses were used to assess the relationship between dietary levels of EEP or RP on performance and intestinal microbiota (p < 0.05). In the trial 1, results showed that the EEP did not cause any significant (p > 0.05) modification in the performance and caecal microbiota. In the trial 2, RP inclusion did not affect the performance but changed the bacterial composition (p < 0.05). Clostridiaceae, Gammaproteobacteria excluding Enterobacteriaceae and Lactobacillus spp. showed a quadratic response (p < 0.05), with the lowest value predicted to occur at 240 ppm, 221 ppm and 213 ppm of RP respectively. The proportion of Bacteroidaceae and Gammaproteobacteria did not differ (p > 0.05) among the experimental groups. The inclusion of ethanolic extract of propolis did not affect the performance and intestinal microbiota, whereas the supplementation of raw propolis modulates the caecal microbiota composition without any effects on chicken performance.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 94%

See 1 more Smart Citation

Caecal microbiota of chickens fed diets containing propolis

Eyng

Murakami

Pedroso³

et al. 2016

Animal Physiology Nutrition

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“…Many researchers have reported that diets supplemented with propolis affected body weight gain, feed intake, and FCR of broilers positively (Roodsari et al, 2004;Shalmany and Shivazad, 2006;Tekeli et al, 2011;Seven et al, 2012;Attia et al, 2014). In contrast to these researchers, Duarte et al (2014) reported that propolis did not affect broiler performance. Acikgöz et al (2005) and Danesgmand et al (2015) reported a decrease in the growth performance of broilers fed diets supplemented with propolis and EEP, respectively.…”

Section: Discussionmentioning

confidence: 98%

Effects of raw propolis or water and ethanol extracts of propolis on performance, immune system, and some blood parameters of broiler breeders

Şahin

Öztürk

2018

R. Bras. Zootec.

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The objective of this study was to determine the effects of raw bee propolis and water or ethanol extract of propolis on growth performance, some blood parameters, and immunoglobulins in 15-20-week-old Ross-308 broiler breeders. The birds in the control were fed a diet without propolis, whereas the birds in the treatment groups were fed diets with raw propolis (RP), water (WEP), and ethanol (EEP) extract of propolis at the level of 1200, 400, and 400 ppm, respectively. Raw propolis and propolis extracts did not affect body weight gain, feed intake, feed conversion ratio, and some blood parameters, such as aspartate aminotransferase, alanine aminotransferase, total antioxidant status, triglyceride, and phosphorus. Birds fed EEP and those in control group had a lower IgA value compared with birds fed RP. Birds fed RP had higher IgM level than those of the other groups, and birds fed EEP had lower IgM level than those of control and RP-fed group. The IgY value of breeders fed EEP was higher than those of the other treatment birds, whereas that of WEP-fed birds was higher than those of control and RP treatment. The antibody levels of Anti-Newcastle disease virus and anti-infectious bursal disease virus were higher in EEP and RP-fed groups than those in the control and WEP-fed groups. The WEP decreased total oxidant status value compared with the control and RP treatments, whereas EEP and WEP increased plasma total protein and calcium contents compared with the control. The EEP increased plasma albumin content compared with RP. The addition of propolis extracts, especially WEP and EEP, to diet improves immunity and antioxidant activity, as well as enhances Ca absorption of broiler breeders.

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“…It is interesting that in broilers fed crude propolis, only crypts in ileum were affected and authors did not recommend the crude propolis supplementation in broiler diets from 1 to 21 days of age. 31 Quantitative morphometric analysis showed also significant increase in the intestinal epithelium relative volume in group with medium (group L) and high (group H) concentrations of bee pollen. The relative volume of lamina propria decreased in both groups when compared to the control.…”

Section: Discussionmentioning

confidence: 99%

Changes in Intestinal Morphology of Rats Fed with Different Levels of Bee Pollen

Toman¹,

Hájková²,

Hluchý³

2015

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Context: Bee pollen has been related to therapeutic properties including antibiotic, antineoplasic, antidiarrhoeic and antioxidant activities. However, little is known about the effect of pollen feeding on the intestinal structures and functions. Aims: The objective of this study was to evaluate the small intestine morphology in rats fed with three different pollen levels. Materials and Methods: Forty rats were randomly separated into 4 groups of 10 rats each. Control group (C) was fed with a basic diet, group L received a diet supplemented with 0.2% (w/w), group M with 0.5% (w/w) and group H with 0.75% (w/w) bee pollen for 90 days. The relative volume of intestinal mucosa structures, length of villi and Lieberkühn crypts formation were evaluated. Results: Quantitative morphometry and histology methods revealed significant increase in the relative volume of epithelium (P<0.0001) and decrease in the connective tissue volume (P<0.0001) of jejunum in groups M and H as compared to the control. The intestinal villi length significantly (P<0.0001) increased in all experimental groups. On the other hand, the Lieberkühn crypts depth significantly (P<0.0001) increased only in groups L and M but decreased (P<0.0001) in the highest pollen-exposed group H. Conclusion: The most significant results were recorded in the medium pollen exposed group. We conclude that the bee pollen concentration of 0.5% is the most effective in the increase of relative volume of the intestinal epithelium and villi length. The results demonstrated that bee pollen affects the small intestine development in a concentration-dependent manner and could be beneficial for intestinal function.Key words: Bee pollen, Histology, morphometry, Rat, Small intestine. PICTORIAL ABSTRACT SUMMARY• Bee pollen enhances the develop ment of the small intestine in rats from 4 weeks to 4 months of age • Intestinal epithelium volume, villi length and crypts depth increase.• Bee pollen acts in a concentration-dependent manner.Abbreviations used: w/w: Percent Concentration Weight/Weight.

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Intestinal morphology and activity of digestive enzymes in broilers fed crude propolis

Cited by 10 publications

References 48 publications

Caecal microbiota of chickens fed diets containing propolis

Caecal microbiota of chickens fed diets containing propolis

Effects of raw propolis or water and ethanol extracts of propolis on performance, immune system, and some blood parameters of broiler breeders

Changes in Intestinal Morphology of Rats Fed with Different Levels of Bee Pollen

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