Changes in Intestinal Morphology of Rats Fed with Different Levels of Bee Pollen

Toman, R.; Hájková, Zuzana; Hluchý, Svätoslav

doi:10.5530/pc.2015.4.8

Cited by 8 publications

(9 citation statements)

References 28 publications

(31 reference statements)

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“…Moreover, crypts depth was significantly deeper in the Pr group. These results are consistent with another study [ 50 ], and confirm the positive effect of dietary propolis supplementation on the intestinal morphology and mucosal development. Therefore, the overall histomorphometric changes induced by propolis could be remarkably useful, particularly during heat stress, when the intestinal mucosa is damaged and requires a rapid renewal of villus cells [ 51 ].…”

Section: Discussionsupporting

confidence: 93%

The importance of propolis in alleviating the negative physiological effects of heat stress in quail chicks

et al. 2017

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Heat stress is one of the most detrimental confrontations in tropical and subtropical regions of the world, causing considerable economic losses in poultry production. Propolis, a resinous product of worker honeybees, possesses several biological activities that could be used to alleviate the deleterious effects of high environmental temperature on poultry production. The current study was aimed at evaluating the effects of propolis supplementation to Japanese quail (Coturnix coturnix japonica) diets on the production performance, intestinal histomorphology, relative physiological and immunological parameters, and selected gene expression under heat stress conditions. Three hundred one-day-old Japanese quail chicks were randomly distributed into 20 wired-cages. At 28 d of age, the birds were divided into 2 temperature treatment groups; a normal at 24°C (C group) and a heat stress at 35°C (HS group). The birds in each group were further assigned to 2 subgroups; one of them was fed on a basal diet without propolis supplementation (-Pr subgroup) while the other was supplemented with propolis (+Pr subgroup). Production performance including body weight gain, feed intake and feed conversion ratio were measured. The intestinal histomorphological measurements were also performed for all treatment groups. Relative physiological parameters including body temperature, corticosterone hormone level, malondialdehyde (MDA) and free triiodothyronine hormone (fT3), as well as the relative immunological parameters including the total white blood cells count (TWBC’s), heterophil/lymphocyte (H/L) ratio and lymphocyte proliferation index, were also measured. Furthermore, the mRNA expression for toll like receptor 5 (TLR5), cysteine-aspartic protease-6 (CASP6) and heat shock proteins 70 and 90 (Hsp70 and Hsp90) genes was quantified in this study. The quail production performance was significantly (P<0.05) impaired by HS treatment, while Pr treatment significantly improved the quail production performance. The villus width and area were significantly (P<0.05) lower in the HS compared to the C group, while Pr treatment significantly increased crypts depth of quail. A negative impact of HS treatment was observed on the physiological status of quail; however, propolis significantly alleviated this negative effect. Moreover, quail of the HS group expressed lower immunological parameters than C group, while propolis enhanced the immune status of the quail. The relative mRNA expression of TLR5 gene was down-regulated by HS treatment while it was up-regulated by the Pr treatment. Furthermore, the positive effects of propolis in HS-quail were evidenced by normalizing the high expressions of CASP6 and Hsp70 genes when compared to the C group. Based on these results, the addition of propolis to quail diets as a potential nutritional strategy in order to improve their performance, especially under heat stress conditions, is recommended.

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Section: Discussionsupporting

confidence: 93%

The importance of propolis in alleviating the negative physiological effects of heat stress in quail chicks

et al. 2017

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“…The small intestine is an important organ responsible for the digestion and absorption of nutrients from the diet. Any changes in its function affect the function of other organs and systems in the organism [15]. There are only a few studies that have previously evaluated the effect of propolis and bee pollen on the intestinal morphology of broiler chickens, and their results are contradictory [12,16,17,18,19].…”

Section: Introductionmentioning

confidence: 99%

Intestinal Morphology in Broiler Chickens Supplemented with Propolis and Bee Pollen

Prakatur

Miškulin

Pavić

et al. 2019

Animals

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The aim of this study was to determine the influence of dietary supplementation with propolis and bee pollen on the intestinal morphology and absorptive surface areas of chickens. Two hundred day-old Ross 308 chickens (100 male and 100 female) were equally allocated into five groups. Throughout the whole study, the control group of chickens was fed with a basal diet, while the experimental groups of chickens were fed with the same diet supplemented with propolis and bee pollen: P1 = 0.25 g of propolis/kg + 20 g of bee pollen/kg; P2 = 0.5 g of propolis/kg; P3 = 1.0 g of propolis/kg; P4 = 20 g of bee pollen/kg. The duodenal villi of chickens from all experimental groups were significantly higher and wider (p < 0.001), while their duodenal villi crypts were significantly deeper (p < 0.001) in comparison with these parameters in chickens from the control group. The villus height to crypt depth ratio, as well as the absorptive surface areas of broiler chickens, were significantly increased (p < 0.001) in experimental groups of chickens in comparison with the control group. These findings suggest that dietary supplementation with propolis and bee pollen has a beneficial effect on broilers chickens’ intestinal morphophysiology.

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“…One reason could be the stimulation of digestive enzymes by HBPE, which are essential factors in the digestion and absorption of nutrients. Stimulation of digestive enzymes and the modulating digestive organ by HBPE have been reported in other animals (Abdelnour et al, 2019; Fazayeli‐Rad, Afzali, Farhangfar, & Asghari, 2015; Toman, Hajkova, & Hluchy, 2015; Wang, Li, Wang, Xin, & Wang, 2007). In our previous studies, we observed that garlic ( Allium sativum ) (Esmaeili et al., 2017a) and barberry root extract (Ramezanzadeh, Abedian Kenari, Esmaeili, & Rombenso, 2020) could stimulate digestive enzymes.…”

Section: Discussionmentioning

confidence: 69%

Effects of hydroalcoholic extract of honeybee pollen on growth performance, flesh quality, and immune and stress response response of rainbow trout (Oncorhynchus mykiss)

et al. 2020

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The effects of dietary hydroalcoholic extract of honeybee pollen (HBPE) on growth performance, flesh quality, immunity and stress response were investigated. Two hundred fifty‐five rainbow trout (11.14 ± 1.06 g) were fed five diets containing increasing levels of HBPE: control, 0.25HBPE (0.25 g HBPE/kg), 0.5HBPE (0.5 g HBPE/kg), 1HBPE (1 g HBPE/kg) and 1.5HBPE (1.5 g HBPE/kg). After 56 days, rainbow trout fed the 0.5HBPE and 1HBPE diets displayed significantly higher weight gain (49.58 and 53.25 g) and protein efficiency (2.88 and 2.83) compared to those fed the control diet (4.89 and 2.05), respectively (p < .05). For flesh quality, higher protein content in the whole body of fish fed the 1HBPE (633.3 g/kg) and 1.5HBPE (640.9 g/kg) diets was observed when compared to other groups (p < .05). There were no significant differences in the saturated and monounsaturated fatty acid content. Individuals fed the 1HBPE (234.5 g/kg) and 1.5HBPE (234.7 g/kg) diets exhibited higher levels of n‐3 long‐chain polyunsaturated fatty acids in the muscle than the other groups (p < .05). In terms of the immune system, the serum lysozyme and alternative complement pathway haemolytic activity levels in the 0.5HBPE (47.66 and 148.00 U/ml) and 1HBPE (46.00 and 146.33 U/ml) treatments were higher than the other treatments (p < .05). When Aeromonas hydrophila was exposed to different dosages of HBPE, a higher inhibitory zone resulted from 1,000 and 1,500 mg/ml dosage. The lower levels of plasma cortisol were observed in the 1HBPE and 1.5HBPE groups after the complement stress test. Collectively, the present findings suggest that the 1HBPE diet supported superior growth, flesh quality, immunity and stress response of rainbow trout.

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Changes in Intestinal Morphology of Rats Fed with Different Levels of Bee Pollen

Cited by 8 publications

References 28 publications

The importance of propolis in alleviating the negative physiological effects of heat stress in quail chicks

The importance of propolis in alleviating the negative physiological effects of heat stress in quail chicks

Intestinal Morphology in Broiler Chickens Supplemented with Propolis and Bee Pollen

Effects of hydroalcoholic extract of honeybee pollen on growth performance, flesh quality, and immune and stress response response of rainbow trout (Oncorhynchus mykiss)

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