Intestinal explant barrier chip: long-term intestinal absorption screening in a novel microphysiological system using tissue explants

Amirabadi, Hossein Eslami; Donkers, Joanne M.; Wierenga, Esmée; Ingenhut, Bastiaan; Pieters, Lisanne; Stevens, Lianne; Donkers, Tim; Westerhout, Joost; Masereeuw, Rosalinde; Bobeldijk, I.; Nooijen, Irene; Steeg, Evita van der

doi:10.1039/d1lc00669j

Cited by 27 publications

(39 citation statements)

References 84 publications

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“…33,65 Optical biosensors Optical biosensors are desirable for monitoring of MPSs without the need for electrical wires. [66][67][68][69] They can quantitatively measure analytes of interest and provide information on molecular interactions. Optical biosensors generally involve monitoring the changes in luminescence intensity, reflective index, and angles of incident/reflected lights.…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Integrated biosensors for monitoring microphysiological systems

et al. 2022

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Section: Electrochemical Biosensorsmentioning

confidence: 99%

Integrated biosensors for monitoring microphysiological systems

et al. 2022

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“…This device includes modules for biopsy removal from the acquisition tool, transport, staining and rinsing, imaging, segmentation, and multiplexed storage. Eslami Amirabadi et al developed a microphysiological system that uses tissue explants with intact intestinal architecture and cell type diversity and creates a dynamic microfluidic microenvironment that prolongs tissue viability . This approach benefits from the complexity of tissue explants and the flow in microfluidic chips to overcome the short viability of tissue explant in static conditions and provide a more accurate model than in vitro ones.…”

Section: Tissue Organ Organoids and Whole Organismsmentioning

confidence: 99%

Advances in Microfluidics: Technical Innovations and Applications in Diagnostics and Therapeutics

Aubry

Lee

2023

Anal. Chem.

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“…However, it is not possible to keep the explant tissue alive for a long period in a static environment. Amirabadi and his colleagues developed an intestinal explant barrier chip (IEBC) to analyze intestinal permeability ex vivo [ 378 ]. The novel platform incorporated human and porcine intestinal colon tissue explants in separate microchannels to study the intestinal absorption of therapeutics in a dynamic microenvironment with small non-specific binding of therapeutic molecules.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Biomedical Applications of Microfluidic Devices: A Review

et al. 2022

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Both passive and active microfluidic chips are used in many biomedical and chemical applications to support fluid mixing, particle manipulations, and signal detection. Passive microfluidic devices are geometry-dependent, and their uses are rather limited. Active microfluidic devices include sensors or detectors that transduce chemical, biological, and physical changes into electrical or optical signals. Also, they are transduction devices that detect biological and chemical changes in biomedical applications, and they are highly versatile microfluidic tools for disease diagnosis and organ modeling. This review provides a comprehensive overview of the significant advances that have been made in the development of microfluidics devices. We will discuss the function of microfluidic devices as micromixers or as sorters of cells and substances (e.g., microfiltration, flow or displacement, and trapping). Microfluidic devices are fabricated using a range of techniques, including molding, etching, three-dimensional printing, and nanofabrication. Their broad utility lies in the detection of diagnostic biomarkers and organ-on-chip approaches that permit disease modeling in cancer, as well as uses in neurological, cardiovascular, hepatic, and pulmonary diseases. Biosensor applications allow for point-of-care testing, using assays based on enzymes, nanozymes, antibodies, or nucleic acids (DNA or RNA). An anticipated development in the field includes the optimization of techniques for the fabrication of microfluidic devices using biocompatible materials. These developments will increase biomedical versatility, reduce diagnostic costs, and accelerate diagnosis time of microfluidics technology.

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Intestinal explant barrier chip: long-term intestinal absorption screening in a novel microphysiological system using tissue explants

Abstract: The majority of intestinal in vitro screening models use cell lines that do not reflect the complexity of the human intestinal tract and hence often fail to accurately predict intestinal...

Cited by 27 publications

References 84 publications

Integrated biosensors for monitoring microphysiological systems

Integrated biosensors for monitoring microphysiological systems

Advances in Microfluidics: Technical Innovations and Applications in Diagnostics and Therapeutics

Biomedical Applications of Microfluidic Devices: A Review

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