Advances in Microfluidics: Technical Innovations and Applications in Diagnostics and Therapeutics

Aubry, Guillaume; Lee, H.; Lu, Hang

doi:10.1021/acs.analchem.2c04562

Cited by 30 publications

(12 citation statements)

References 195 publications

(374 reference statements)

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“…As a result, the reaction can replicate hundreds of times within a short time frame, yielding a large amount of single-stranded DNA molecules up to 10 9 copies. 36 So far, RCA has been widely used and improved, and can be combined with other platforms like lateral flow dipsticks, 37,38 microfluidics 39 or CRISPR/Cas systems 40–42 to detect various pathogenic microorganisms. It can also serve as the central design principle of biosensors.…”

Section: Rolling Circle Amplification (Rca)mentioning

confidence: 99%

Advances in isothermal nucleic acid amplification methods for hepatitis B virus detection

Li,

Song,

et al. 2023

Analyst

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Section: Rolling Circle Amplification (Rca)mentioning

confidence: 99%

Advances in isothermal nucleic acid amplification methods for hepatitis B virus detection

Li,

Song,

et al. 2023

Analyst

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“…The field of microfluidics has advanced greatly over the past few decades largely due to key advantages offered by miniaturized device designs . Microfluidic devices are usually more cost-effective to fabricate, easier to transport, and capable of integrating complex operations (such as micromixing) more effectively than their large-scale counterparts.…”

Section: Introductionmentioning

confidence: 99%

“…The field of microfluidics has advanced greatly over the past few decades largely due to key advantages offered by miniaturized device designs. 23 Microfluidic devices are usually more cost-effective to fabricate, easier to transport, and capable of integrating complex operations (such as micromixing) more effectively than their large-scale counterparts. Though extensive research efforts toward cell size-based sorting have been made, 24 efforts for sorting islets and other cell clusters by size have been relatively more limited.…”

Section: ■ Introductionmentioning

confidence: 99%

On Chip Sorting of Stem Cell-Derived β Cell Clusters Using Traveling Surface Acoustic Waves

Sethia,

Rao,

Khashim

et al. 2024

Langmuir

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There is a critical need for sorting complex materials, such as pancreatic islets of Langerhans, exocrine acinar tissues, and embryoid bodies. These materials are cell clusters, which have highly heterogeneous physical properties (such as size, shape, morphology, and deformability). Selecting such materials on the basis of specific properties can improve clinical outcomes and help advance biomedical research. In this work, we focused on sorting one such complex material, human stem cell-derived β cell clusters (SC-β cell clusters), by size. For this purpose, we developed a microfluidic device in which an image detection system was coupled to an actuation mechanism based on traveling surface acoustic waves (TSAWs). SC-β cell clusters of varying size (∼100−500 μm in diameter) were passed through the sorting device. Inside the device, the size of each cluster was estimated from their bright-field images. After size identification, larger clusters, relative to the cutoff size for separation, were selectively actuated using TSAW pulses. As a result of this selective actuation, smaller and larger clusters exited the device from different outlets. At the current sample dilutions, the experimental sorting efficiency ranged between 78% and 90% for a separation cutoff size of 250 μm, yielding sorting throughputs of up to 0.2 SC-β cell clusters/s using our proof-of-concept design. The biocompatibility of this sorting technique was also established, as no difference in SC-β cell cluster viability due to TSAW pulse usage was found. We conclude the proof-of-concept sorting work by discussing a few ways to optimize sorting of SC-β cell clusters for potentially higher sorting efficiency and throughput. This sorting technique can potentially help in achieving a better distribution of islets for clinical islet transplantation (a potential cure for type 1 diabetes). Additionally, the use of this technique for sorting islets can help in characterizing islet biophysical properties by size and selecting suitable islets for improved islet cryopreservation.

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“…[17][18][19] Epidermal microfluidics, due to its enclosed structure, can prevent sweat contamination and evaporation. [20] Nevertheless, its sweat collection efficiency is comparatively low, requiring microchannels to be filled for the sensing section. [21][22][23][24] The resulting mixing of fresh and old sweat in these channels increases the risk of sample contamination due to prolonged collection times.…”

Section: Introductionmentioning

confidence: 99%

Multi‐Bioinspired Droplet Self‐Actuated Sweat Transport Platform for Continuous Wearable Biochemical Monitoring

Shen,

Pang,

Yuan

et al. 2024

Adv Materials Technologies

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Wearable sweat sensors exhibit significant promise in the realm of smart healthcare. Efficient and non‐contaminating continuous sweat collection, transport, and disposal are crucial for enhancing detection accuracy. In this study, a novel wearable sweat sensor is presented that integrates various biomimetic structures, including cactus‐inspired spine, shorebird‐inspired conical through‐holes, and frog‐foot‐inspired micro‐grooves. These structures facilitate sweat droplets to be automatically transported from the skin to the sensing chamber and discharged without external power. First, theoretical analysis, simulation, and experiments based on self‐driven droplet mechanisms to determine the optimal transport efficiency parameters for biomimetic structures are conducted. Next, a novel sweat transport module is fabricated through ultra‐precision 3D printing according to these optimal parameters. And this module has excellent sweat collection performance contrastively. Finally, the novel biomimetic sweat transport module is integrated with a high‐sensitivity glucose detection electrode, resulting in a wearable sweat glucose detection system. Human experiments monitoring glucose metabolism in sweat verify the correlation between glucose concentrations in blood and sweat, which proves the feasibility of reflecting changes in the body's circulatory system by monitoring changes in sweat composition.

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Advances in Microfluidics: Technical Innovations and Applications in Diagnostics and Therapeutics

Abstract: Figure 2. On-chip fluid control. (A) Domino valve for capillary circuits is a combination of retention valve (RV) and stop valve (SV), built in 3D.

Cited by 30 publications

References 195 publications

Advances in isothermal nucleic acid amplification methods for hepatitis B virus detection

Advances in isothermal nucleic acid amplification methods for hepatitis B virus detection

On Chip Sorting of Stem Cell-Derived β Cell Clusters Using Traveling Surface Acoustic Waves

Multi‐Bioinspired Droplet Self‐Actuated Sweat Transport Platform for Continuous Wearable Biochemical Monitoring

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