Interpretations of Voltammetry in the Striatum Based on Chromatography of Striatal Dialysate

Justice, Joseph B.; Wages, Sherry A.; Michael, Adrian C.; Blakely, Randy D.; Neill, Darryl B.

doi:10.1080/01483918308064897

Cited by 34 publications

(9 citation statements)

References 16 publications

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“…They also show that in vivo dialysis can be used in the same animals to monitor concurrently changes in specific brain regions. The dialysis procedure, like that recently described by Justice et al (1983), involves the insertion of the dialysis probe into a previously implanted guide cannula. Therefore, the animals can be readily studied after allowing them a number of days to recover from operative procedures.…”

Section: Discussionmentioning

confidence: 99%

Monitoring the Effect of a Tryptophan Load on Brain Indole Metabolism in Freely Moving Rats by Simultaneous Cerebrospinal Fluid Sampling and Brain Dialysis

Hutson¹,

Sarna²,

Kantamaneni³

et al. 1985

Journal of Neurochemistry

120

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Rats were given L-tryptophan, 50 mg/kg i.p., and its concentration in the CNS was monitored in individual freely moving animals using repeated sampling of cisternal CSF and concurrent striatal dialysis. The 5-hydroxytryptamine metabolite 5-hydroxyindoleacetic acid (5-HIAA) was also measured. Results were compared with changes of central tryptophan and 5-HIAA concentrations in brains of rats killed at various times after administration of L-tryptophan, 50 mg/kg i.p. Tryptophan changes in CSF were proportionate to those in whole brain and followed essentially identical time courses. Results for the striatal dialysate and whole striatum also paralleled each other. Similarly, results for 5-HIAA showed proportionality between CSF and brain and between dialysate and striatum. The data obtained were used to determine pharmacokinetic data for individual rats, i.e., areas under curves for both tryptophan and 5-HIAA and half-lives for the decline of tryptophan. Kinetic parameters varied considerably from rat to rat. However, mean half-lives for tryptophan in CSF, brain, dialysate, and striatum were all comparable. Results in general show the value of repeated CSF sampling and intracerebral dialysis for concurrent monitoring of changes of indole metabolism in the whole brain and a specific brain region, respectively. The methods should be suitable for the continuous monitoring of changes of central transmitter metabolism in parallel with observation of behavior following environmental or dietary changes or drug administration. They also should be of use in the investigation of drug kinetics in the CNS.

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Section: Discussionmentioning

confidence: 99%

Monitoring the Effect of a Tryptophan Load on Brain Indole Metabolism in Freely Moving Rats by Simultaneous Cerebrospinal Fluid Sampling and Brain Dialysis

Hutson¹,

Sarna²,

Kantamaneni³

et al. 1985

Journal of Neurochemistry

120

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“…Microdialysis probes roughly equivalent to previous concentric-style designs (Justice et al, 1983;Hernandez et al, 1986) were constructed from fused silica tubing, 0.5-in sections of 23-gauge (0.025 in 0.d. X 0.01 3 in i.d.)…”

Section: Microdialysismentioning

confidence: 99%

The Anxiogenic β‐Carboline FG 7142 Selectively Increases Dopamine Release in Rat Prefrontal Cortex as Measured by Microdialysis

Bradberry

Lory

Roth

1991

Journal of Neurochemistry

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The effect of the anxiogenic beta-carboline methyl-beta-carboline-3-carboxyamide (FG 7142) on dopamine release in prefrontal cortex and striatum in the awake freely moving rat was determined using the technique of microdialysis. FG 7142 (25 mg/kg, i.p.) caused a time-dependent increase in dopamine release in prefrontal cortex which was statistically significantly greater than the response to vehicle administration. Dopamine release in striatum was unaltered by FG 7142. Pretreatment of animals with the benzodiazepine antagonist Ro 15-1788 (30 mg/kg, i.p., 15 min prior to FG 7142 administration) completely abolished the increase in dopamine release caused by FG 7142 in prefrontal cortex. These data indicate that the anxiogenic benzodiazepine inverse agonist FG 7142 can selectively increase dopamine release in prefrontal cortex, and that this effect appears to be mediated via the gamma-aminobutyric acid/benzodiazepine receptor complex.

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“…To aid in the interpretation of voltammetric data obtained in vivo, a series of experiments were initiated to obtain extracellular fluid (ECF) from local structures in the brain. The various electroactive species were separated by high-performance liquid chromatography (HP-LC) and quantitated with electrochemical detection (1)(2)(3). It soon became evident that chromatographic analysis of the ECF was a method with utility beyond the original purpose of validation of voltammetry in vivo.…”

mentioning

confidence: 99%

Sampling considerations for on-line microbore liquid chromatography of brain dialysate

Wages¹,

Church²,

Justice³

1986

Anal. Chem.

166

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A push-pull dialysis perfusion system for monitoring neurotránsmitter release In deep structures of the brain of freely moving animals Is characterized. The Internal volume of the system has been reduced so that very slow perfusion rates can be used. These slow rates yield high recoveries and small sample volumes, allowing microbore columns with their Inherent Increase In mass sensitivity to be Incorporated into the chromatographic system. Recovery of sample exceeded 90% at 0.1 qL/mln at 37 °C. The maximum sample recovery per minute occurred at approximately 2 gL/mln. The contribution of the perfusion system to sample dispersion was found to be Independent of perfusion rate above 0.1 gL/mln. The detection limit for the neurotransmitter dopamine was 300 fg with a S/N of 2. The method Is suitable for sampling low-molecular-weight molecules In most brain structures.Chemical analysis inside the living brain has long been a difficult problem. Obtaining chemical information with

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Interpretations of Voltammetry in the Striatum Based on Chromatography of Striatal Dialysate

Abstract: Journal of Liquid ChromatographyPublication details, including instructions for authors and subscription information:

Cited by 34 publications

References 16 publications

Monitoring the Effect of a Tryptophan Load on Brain Indole Metabolism in Freely Moving Rats by Simultaneous Cerebrospinal Fluid Sampling and Brain Dialysis

Monitoring the Effect of a Tryptophan Load on Brain Indole Metabolism in Freely Moving Rats by Simultaneous Cerebrospinal Fluid Sampling and Brain Dialysis

The Anxiogenic β‐Carboline FG 7142 Selectively Increases Dopamine Release in Rat Prefrontal Cortex as Measured by Microdialysis

Sampling considerations for on-line microbore liquid chromatography of brain dialysate

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