International Committee on Systematic Bacteriology: Announcement of the Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics

Wayne, Lawrence G.

doi:10.1016/s0723-2020(88)80020-1

Cited by 1,073 publications

(1,213 citation statements)

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“…EzTaxon analysis and the phylogenetic tree showed that strain MBRL 353 T formed a distinct lineage most closely related to R. triatomae IMMIB RIV-085 T and R. equi DSM 20307 T (Fig. 1) (Wayne et al, 1987).…”

Section: Rhodococcus Kyotonensis Ds472 T (Ab269261)mentioning

confidence: 99%

Rhodococcus canchipurensis sp. nov., an actinomycete isolated from a limestone deposit site

Salam

Sanasam

Zheng

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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A novel actinobacterial strain, MBRL 353T, was isolated from a sample collected from a limestone quarry at Hundung, Manipur, India. Comparison of 16S rRNA gene sequences of strain MBRL 353T and other members of the genus Rhodococcus showed sequence similarities ranging from 95.5 to 98.2 %, with strain MBRL 353T showing closest sequence similarity to Rhodococcus triatomae IMMIB RIV-085T (98.2 %) and Rhodococcus equi DSM 20307T (97.2 %). DNA–DNA hybridization results, however, revealed that DNA–DNA relatedness values between strain MBRL 353T and R. triatomae DSM 44892T (43.4 %) and R. equi DSM 20307T (33.4 %) were well below the 70 % limit for species identification. Strain MBRL 353T contained meso-diaminopimelic acid as the diagnostic diamino acid and galactose and arabinose in the cell wall. Mycolic acids were present. The major fatty acids were C16 : 0 (45.7 %), C18 : 1ω9c (18.2 %) and 10-methyl C18 : 0 (11.3 %). The only menaquinone detected was MK-8(H2), while the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unknown phospholipid. The G+C content of the genomic DNA was 69.2 mol%. The phenotypic and genotypic data showed that strain MBRL 353T merits recognition as a representative of a novel species of the genus Rhodococcus for which the name Rhodococcus canchipurensis sp. nov. is proposed; the type strain is MBRL 353T ( = KCTC 19851T = JCM 17578T).

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Section: Rhodococcus Kyotonensis Ds472 T (Ab269261)mentioning

confidence: 99%

Rhodococcus canchipurensis sp. nov., an actinomycete isolated from a limestone deposit site

Salam

Sanasam

Zheng

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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“…Levels of DNA-DNA relatedness between strain YIM kkny16 T and the above two type strains were 25.8 and 20.2 %, respectively. These values were far below the threshold value of 70 % recommended by Wayne et al (1987) for assigning strains to the same species. It is therefore evident, based on the phylogenetic analysis and DNA-DNA hybridization data, that strain YIM kkny16 T does not belong to either V. marismortui or V. halodenitrificans.…”

mentioning

confidence: 63%

“…V. olivae DSM 18098 T shared 99.8 % 16S rRNA gene sequence similarity with V. marismortui DSM 10037 T , and these two strains formed a sub-branch in the phylogenetic tree with significant bootstrap support (100 %) (Fig. 2); however, the level of DNA-DNA relatedness (46.5 %) between the two strains was well below the threshold value recommended by Wayne et al (1987). Although the level of 16S rRNA gene sequence similarity of 97.3 % between strain YIM kkny16 T and V. olivae DSM 18098 T was slightly higher than the threshold value of ¢97 % recommended by Stackebrandt & Goebel (1994) for assigning strains to the same species, the unattached status of the branches occupied by strain YIM kkny16 T and V. olivae DSM 18098 T in the phylogenetic tree (Fig.…”

mentioning

confidence: 89%

Virgibacillus kekensis sp. nov., a moderately halophilic bacterium isolated from a salt lake in China

Chen

Cui

Fritze

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A Gram-positive, moderately halophilic, motile, strictly aerobic, endospore-forming, oxidase-and catalase-positive, rod-shaped bacterium, strain YIM kkny16 T , was isolated from a saline mud sample collected from the Keke salt lake in the Qaidam Basin, north-west China. This isolate grew in the presence of 0-25 % (w/v) NaCl and at pH 6.0-10.0 and 10-50 6C; optimum growth was observed with 10 % (w/v) NaCl and at pH 7.0 and 37 6C. Strain YIM kkny16 T had meso-diaminopimelic acid as the diagnostic diamino acid, MK-7 as the predominant respiratory quinone, with a significant amount of MK-6, and anteiso-C 15 : 0 , iso-C 14 : 0 and C 16 : 1 v7c alcohol as major fatty acids. Major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content was 41.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain YIM kkny16 T was a member of the genus Virgibacillus, exhibiting sequence similarities of 94.9-97.3 % to the type strains of recognized Virgibacillus species. Strain YIM kkny16 T could be differentiated from recognized Virgibacillus species based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA-DNA hybridization data. On the basis of evidence from this polyphasic study, strain YIM kkny16 T is considered to represent a novel species of the genus Virgibacillus, for which the name Virgibacillus kekensis sp. nov. is proposed. The type strain is YIM kkny16 T (5DSM 17056 T 5CGMCC 1.6298 T ).At the time of writing, the genus Virgibacillus comprises 11 recognized species, with Virgibacillus olivae (Quesada et al., 2007) and Virgibacillus halophilus (An et al., 2007) being the most recently described. Virgibacillus pantothenticus (Heyndrickx et al., 1998) is the type species. During a recent study of the microbial diversity of the Keke salt lake in the Qaidam Basin of Qinghai Province, north-west China, a moderately halophilic rod-shaped bacterium, designated strain YIM kkny16 T , was isolated from a saline mud sample. Phenotypic and chemotaxonomic characterization, together with phylogenetic analysis based on 16S rRNA gene sequences, showed that strain YIM kkny16 T was related to members of the genus Virgibacillus, but represented a novel species.Strain YIM kkny16 T was isolated from a saline mud sample by plating 1 : 10 serial dilutions of the sample on Difco marine agar 2216 (MA; pH 7.2) at 28 u C for 2 weeks. The strain was maintained both in Difco marine broth (MB) supplemented with 20 % (v/v) glycerol at 280 u C and as lyophilized cultures at 4 u C. Unless otherwise indicated, morphological and physiological studies were performed with cells grown on MA supplemented with 10 % (w/v) NaCl, and on ISP medium 2 agar (Shirling & Gottlieb, 1966), nutrient agar and trypticase soy agar (BBL) as controls. Cells for chemotaxonomic and molecular systematic studies were grown in MB supplemented with 10 % (w/v) NaCl in shake flasks (with shaking at aboutThe GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM kkny1...

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“…Finally, DNA relatedness values were recorded as mean±SD values for the remaining three scores (Yoon et al, 2007). DNA-DNA relatedness between strain CS5-AC15 T and A. oligospora JCM 10648 T was 37.9±0.7 %, well below the 70 % cut-off value recommended for the delineation of bacterial genospecies (Wayne et al, 1987).…”

Section: Characteristic 1mentioning

confidence: 94%

Actinomadura rupiterrae sp. nov., isolated from cliff soil

Lee

2012

International Journal of Systematic and Evolutionary Microbiology

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A novel actinomycete strain, designated CS5-AC15 T , was isolated from a soil sample collected from a cliff on Mara Island, Jeju, Republic of Korea, and subjected to a polyphasic taxonomic analysis. The isolate produced well-developed, yellow substrate mycelium and white aerial mycelium that differentiated into straight or flexuous chains of smooth-surfaced spores. 16S rRNA gene sequence analyses showed that the organism belonged to the family Thermomonosporaceae and formed a tight cluster with the type strain of Actinomadura oligospora (97.4 % sequence similarity). Chemotaxonomic characteristics were consistent with its assignment to the genus Actinomadura in that the isolate had meso-diaminopimelic acid as the diagnostic diamino acid in the cell wall, madurose as the characteristic sugar, N-acetyl type of murein in the peptidoglycan, MK-9(H 6 ) and MK-9(H 8 ) as major menaquinones and a polar lipid profile containing diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and unknown phospholipids. Mycolic acids were not detected. The predominant fatty acids were C 16 : 0 , C 18 : 1 v9c and iso-C 16 : 0 . The DNA G+C content was 70.9 mol%. DNA relatedness of strain CS5-AC15 T and A. oligospora JCM 10648 T was 37.9±0.7 %. On the basis of the phenotypic, phylogenetic and DNA-DNA hybridization data, strain CS5-AC15 T is assigned to a novel species of the genus Actinomadura, for which the name Actinomadura rupiterrae sp. nov. is proposed (type strain CS5-AC15 T 5KCTC 19559 T 5DSM 45251 T ).

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International Committee on Systematic Bacteriology: Announcement of the Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics

Cited by 1,073 publications

References 1 publication

Rhodococcus canchipurensis sp. nov., an actinomycete isolated from a limestone deposit site

Rhodococcus canchipurensis sp. nov., an actinomycete isolated from a limestone deposit site

Virgibacillus kekensis sp. nov., a moderately halophilic bacterium isolated from a salt lake in China

Actinomadura rupiterrae sp. nov., isolated from cliff soil

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