Internal necrosis in stored white cabbage caused by turnip mosaic virus

Walkey, D. G. A.; Webb, Matthew J.

doi:10.1111/j.1744-7348.1978.tb05971.x

Cited by 24 publications

(18 citation statements)

References 2 publications

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“…Droplets of crude sap at the various dilutions were placed on parafilm. An electron microscope grid, coated with 1/1000 homologous antiserum (diluted in 0-05 M potassium phosphate buffer, pH 7-5) was washed with the same buffer and its coated surface floated on the sap droplet for 1 h. The grid was then washed in the same buffer and double distilled water before staining in uranyl acetate (Walkey, 1991). After drying, it was examined in the electron microscope.…”

Section: Latex Particle Vba Comparison Testsmentioning

confidence: 99%

An evaluation of a virobacterial agglutination test for the detection of plant viruses

Walkey¹,

Lyons²,

Taylor³

1992

Plant Pathology

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Agglutination of Staphylococcus aureus bacteria particles, conjugated with specific virus antibodies, has been used to identify a wide range of plant viruses in crude sap extracts. The test distinguishes between seven different potyviruses in homologous and heterologous reactions, but does not distinguish different strains of bean yellow mosaic virus. The sensitivity of the virobacterial agglutination (VBA) test compares favourably with virus detection in ISEM particle trapping and local lesion assay and is only slightly less sensitive than direct ELISA tests. The test is more sensitive and simpler to user than latex particle agglutination tests.

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Section: Latex Particle Vba Comparison Testsmentioning

confidence: 99%

An evaluation of a virobacterial agglutination test for the detection of plant viruses

Walkey¹,

Lyons²,

Taylor³

1992

Plant Pathology

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“…Plants infected with TuMV produce a variety of symptoms, including mottling, mosaic, leaf distortion and stunting. Black necrotic spots and ringspots are frequently produced in common cabbage, Chinese cabbage, cauliflower and Brussels sprouts (AVRDC, 1985 ;Tomlinson, 1970 ;Walkey & Webb, 1978), thus greatly reducing marketability.…”

Section: Introductionmentioning

confidence: 99%

Inheritance of resistance to turnip mosaic virus in Chinese cabbage

1993

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Turnip mosaic virus (TuMV) is the most important virus of commercially grown cole crops in many Asian countries, affecting both yield and quality. TuMV-infected Chinese cabbage becomes unmarketable because of the presence of black spots and necrosis often induced by the virus . Resistance breeding is complicated by the existence of five strains of the virus, one of which was discovered in 1985 for the first time in Taiwan . Resistance to strains C1 to C3 is readily available among the Chinese cabbage germplasm at AVRDC, whereas resistance to strains C4 and C5 is rarely found . To elucidate the inheritance of resistance to TuMV, P,, P 2 , F,, F2 and BC, generations of crosses between the resistant line`0-2' and three susceptible lines,`E-7', `E-9' and 'FL-9', were inoculated with strains C4 and C5 . Segregation ratios obtained by visual observation and enzyme-linked immunosorbent assay (ELISA) indicate that two recessive genes confer resistance to both TuMV-C4 and TuMV-C5 .

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“…Turnip mosaic virus (TuMV) is a major disease of white cabbage (Brussica oleraceu var. cupitata L.) in the United Kingdom (Walkey & Webb, 1978;Walkey & Neely, 1980), Europe (Glaeser, 1970;Weidemann, 1981 ;Polak, 1983) and elsewhere (Conroy, 1959;Natti, 1960). The virus may cause severe necrotic symptoms (Plate, fig.…”

Section: Introductionmentioning

confidence: 99%

Reactions of white cabbage (Brassica oleracea var. capitata) to four different strains of turnip mosaic virus

Walkey

Pink

1988

Annals of Applied Biology

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TuMV strains isolated in the UK, Germany, Denmark and Greece have been shown to be biologically distinct by host range studies. The effects of the four strains on symptoms, yield and storage performance of four cultivars of white cabbage were assessed in gauze house experiments. Three of the cultivars, Decema Extra, Vitala and Winter White 111, were known to have high levels of resistance to the UK strain and the fourth, cv. Polinius, is known to be highly susceptible. The resistance to the UK strain previously observed in the three 'resistant cultivars' was generally effective against the other three strains; however, the level of resistance was virus strain dependent and variable for the symptoms of external necrosis, internal necrosis and yield depression. The UK strain caused the highest level of external necrosis and the German strain the highest internal necrosis. These two strains generally caused greater yield reductions than the Danish or Greek strains. Virus infection had no effect upon the incidence of the pepper-spot necrosis disorder. Breeding strategies for handling the observed resistance are discussed. 17-20. Pjlanzenschutzdienst (Braunschweig) 33,

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Internal necrosis in stored white cabbage caused by turnip mosaic virus

Cited by 24 publications

References 2 publications

An evaluation of a virobacterial agglutination test for the detection of plant viruses

An evaluation of a virobacterial agglutination test for the detection of plant viruses

Inheritance of resistance to turnip mosaic virus in Chinese cabbage

Reactions of white cabbage (Brassica oleracea var. capitata) to four different strains of turnip mosaic virus

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