Intermediate dehydrogenase-oxidase form of xanthine oxidoreductase in rat liver

Kamiński, Zbigniew; Jeżewska, Maria

doi:10.1042/bj1810177

Cited by 56 publications

(17 citation statements)

References 13 publications

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“…Blood was collected and ileum tissues were removed for biochemical and histological investigations. XO and XDH were determined according to Kaminski and Jewezska (1979). SOD and CAT activities were determined according to Minami and Yoshikawa (1979) and Aebi (1984), respectively.…”

Section: Methodsmentioning

confidence: 99%

Lycopene protects the structure of the small intestine against gamma‐radiation‐induced oxidative stress

Saada

Rezk

Eltahawy

2010

Phytotherapy Research

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The small intestine displays numerous morphological and functional alterations after exposure to ionizing radiations. Oxidative stress and changes in monoamines levels may contribute toward some of these alterations. The objective of the current work is to evaluate the efficacy of lycopene on radiation-induced damage in the small intestine. Lycopene (5 mg/kg BW) was given to male albino rats, via gavages for 7 days before whole body exposure to gamma ray (6 Gy). Irradiated animals, sacrificed 7 days after irradiation, showed sloughing villi, ulcers, and ruptured goblet cells, shrinkage of submucosa layers, more fibers and fibroblasts. Histopathological changes were associated with a significant increase in thiobarbituric acid reactive substances (TBARS) and alteration in xanthine oxidoreductase system (XOR). In parallel, significant decreases in reduced glutathione (GSH) content, superoxide dismutase (SOD) and catalase (CAT) activities were recorded. Gamma irradiation has also induced a significant decrease in the level of monoamines: serotonin (5-HT), dopamine (DA), norepinephrine (NE), and epinephrine (EPI) associated with an increase in monoamine-oxidase (MAO) activity. Lycopene pretreatment has significantly improved the oxidant/antioxidant status, which was associated with significant regeneration of the small intestine, and improved monoamines levels. Based on these results, it is concluded that lycopene may protect the small intestine against radiation-induced damage.

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Section: Methodsmentioning

confidence: 99%

Lycopene protects the structure of the small intestine against gamma‐radiation‐induced oxidative stress

Saada

Rezk

Eltahawy

2010

Phytotherapy Research

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“…and the supernatants were used to estimate the level of malondialdehyde (MDA) (Yoshioka et al, 1979). The activity of xanthine oxidase (XO) and xanthine dehydrogenase (XDH) (Kaminski and Jewezska, 1979), glutathione content (GSH) (Gross et al, 1967) and the activity of superoxide dismutase (SOD) as (Minami and Yoshikawa, 1979) and catalase (CAT) (Aebi, 1984). Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) were assayed in the liver homogenate by the method of Agarwal and Goedde (1990).…”

Section: Biochemical Analysismentioning

confidence: 99%

Identification of the Therapeutic Effects of Gamma-Irradiated Chamomile Aqueous Extract Against Alcohol Induced Hepato-nephrotoxicity in Rats

el-Megid

Azeem²,

EL-Shahat

et al. 2017

Egypt. J. Rad.Sci.

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“…due to sedimentation of the particular sub fraction of XO [11]; in these conditions, it is preferable to centrifuge at 12,000 g, which is contrary to that which is often recommended [2][3][4]9], Crude nondialyzed homogenates contain large varieties of potential XO activity inhib itors: UA, xanthine, NADH, etc. [5,6]. The increase in enzymatic activity after dialysis confirms the presence of these inhibitors without stating what they are (table II).…”

Section: Preparation Of the Homogenatementioning

confidence: 55%

Measurement of Xanthine Oxidase Activity in Some Human Tissues

Mousson

Desjacques

Baltassat

1983

Enzyme

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Xanthine oxidase activity in human liver or jejunum homogenates was measured by using 8-^14 C-hypoxanthine (HX) as a substrate. Products of oxidation were separated by thin-layer chromatography on MN Polygram cellulose and located by autoradiography. The percentage of oxidation was measured by liquid scintillation. Main parameters of the enzyme reaction have been optimized. Repeatability and reproducibility of the overall procedure give a variation coefficient of 6.3 and 7.7%, respectively. Minimal detectable enzymatic activity corresponds to an oxidation percentage of 1 % (HX) for liver and jejunum. The reliability and practicability of this method allow a rapid diagnosis of xanthine oxidase deficiency. Normal values obtained by this optimized method range between 25 and 42 μkat • kg^-1 protein for liver and 18 and 40 μkat -kg-1 protein for jejunum.

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Intermediate dehydrogenase-oxidase form of xanthine oxidoreductase in rat liver

Abstract: A spectrophotometric method for the determination of three forms of xanthine oxidoreductase, namely dehydrogenase (D), dehydrogenase-oxidase (D/0) and oxidase (0)

Cited by 56 publications

References 13 publications

Lycopene protects the structure of the small intestine against gamma‐radiation‐induced oxidative stress

Lycopene protects the structure of the small intestine against gamma‐radiation‐induced oxidative stress

Identification of the Therapeutic Effects of Gamma-Irradiated Chamomile Aqueous Extract Against Alcohol Induced Hepato-nephrotoxicity in Rats

Measurement of Xanthine Oxidase Activity in Some Human Tissues

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