Measurement of Xanthine Oxidase Activity in
Some Human Tissues

Mousson, B.; Desjacques, P; Baltassat, P

doi:10.1159/000469601

Cited by 40 publications

(15 citation statements)

References 7 publications

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“…The superoxide anion radicals scavenging effect of TFLA was assessed spectrophotometrically as reported previously with a slight modification [18]. Superoxide anions were generated in a nonenzymic phenazine methosulfate-NADH system by following the reduction of nitroblue tetrazolium.…”

Section: Superoxide Anion Radical Scavenging Activity Assaymentioning

confidence: 99%

Hepatoprotective effect of total flavonoids from Laggera alata against carbon tetrachloride-induced injury in primary cultured neonatal rat hepatocytes and in rats with hepatic damage

Wang²,

Zheng

et al. 2006

J Biomed Sci

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SummaryThe hepatoprotective activities of total flavonoids of Laggera alata (TFLA) were evaluated by carbon tetrachloride (CCl 4 )-induced injury in primary cultured neonatal rat hepatocytes and in rats with hepatic damage. In vitro, TFLA at a concentration range of 1-100 lg/ml improved cell viability and inhibited cellular leakage of two enzymes, hepatocyte aspartate aminotransferase (AST) and alanine aminotransferase (ALT), caused by CCl 4 . In vivo, oral treatment with TFLA at doses of 50, 100, and 200 mg/kg significantly reduced the levels of AST, ALT, total protein, and albumin in serum and the hydroxyproline and sialic acid levels in liver. Histopathological examinations revealed that liver damage were improved when treated with TFLA. Meanwhile, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide radicals scavenging activities of TFLA were also determinated. To understand the exact components of TFLA responsible for the hepatoprotective effect, nine flavonoid compounds were isolated and identified from TFLA. In conclusion, the present investigation was the first to verify the hepatoprotective effect of L. alata in vitro and in vivo. The hepatoprotective action of TFLA is likely related to its potent antioxidative and anti-inflammatory activity. Neutralizing reactive oxygen species by nonenzymatic mechanisms and enhancing the activity of original natural hepatic-antioxidant enzymes may be the main mechanisms of TFLA against CCl 4 -induced injury.

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Section: Superoxide Anion Radical Scavenging Activity Assaymentioning

confidence: 99%

Hepatoprotective effect of total flavonoids from Laggera alata against carbon tetrachloride-induced injury in primary cultured neonatal rat hepatocytes and in rats with hepatic damage

Wang²,

Zheng

et al. 2006

J Biomed Sci

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“…This mechanism has been strongly implicated in the pathogenesis of ischemia-reperfusion injury of the small intestine (1 [2][3][4][5][6][7][8][9][10][11][12][13][14][15].…”

mentioning

confidence: 99%

“…The production xanthine and uric acid from (3,19,20). Possible changes in the enzyme activity during the first 24 h after death were evaluated using samples of rabbit liver, and identical storage conditions.…”

mentioning

confidence: 99%

Xanthine Oxidase during Human Fetal Development

Vettenranta

Raivio

1990

Pediatr Res

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ABSTRACT. Through oxygen free radical production, xanthine oxidase (XOD, E.C.1.2.3.2) has been implicated in the pathogenesis of postischemic and hyperoxic tissue injuries among newborn. We measured the activity and evaluated the kinetic characteristics of XOD in human fetal liver, intestine, brain, and myocardium. Both the fetal liver and intestine contain a high XOD activity through gestation. The activity increases in the liver and decreases in the intestine with advancing gestation. The apparent Km for hypoxanthine is 4.8-5.5 pM in the intestine throughout gestation and in the liver at term but higher than 30 pM in the liver during the first half of pregnancy. The activity is undetectable both in the fetal brain and myocardium throughout gestation. Thus, XOD activity is present at least in the liver and intestine to account for the oxidation of hypoxanthine and xanthine. However, direct evidence for adenine nucleotide catabolism, followed by oxidation of the accumulated hypoxanthine during tissue reoxygenation in the human liver or intestine is not available. (Pediatr Res 27: [286][287][288]1990)

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“…However, although the serum allopurinol concentrations recorded in hyperuricaemic subjects are sufficient to inhibit liver xanthine oxidase, they might not suffice to inhibit the xanthine oxidase activity introduced into the reaction medium, an activity about a hundred times that in human liver (13).…”

Section: Discussionmentioning

confidence: 94%

“…Principle [8][9][10][11][12][13][14] C]Inosine S'-monophosph te is choseri s Substrate; it is hydrolysed by 5'*nucleotidase and non-ŝ pecific alkaline phosphatases to yield [8^1 4 C]inosine and inorganic phosphate. Glycerol 2-phosphate acts s "divefsion Substrate", it is preferehtially hydrolysed by non-specific alkaline phosphatases.…”

Section: Inhibition Of Inosine S'-monophosphate Hydrolysismentioning

confidence: 99%

Continuous Assay of Serum 5'-Nucleotidase Activity with Inosine 5'-Monophosphate as Substrate and Total Automation Using a Transfer-Analyzer (Kem-O-Mat)

Stephant¹,

Vernet-Nyssen²,

Mousson³

1983

Clinical Chemistry and Laboratory Medicine

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Summary:The continuous spectröphotometric assay of 5'-nucleotidase originally described by Heinz et al. ((1980) J. Clin. Chem. Clin. Biochem. 18, 781-788) was modified and fully automated on a Kem-O-Mat transfer analyzer, using inosine S'-monophosphate äs Substrate. The reaction product was hydrogen peroxide and the redüction of NADP was observed for 10 minutes at 340 nm and at a reaction temperature of 30 °C. The different factors involved in the enzyme reaction were checked, including the Substrate concentration, reaction rate, linearity and Substrate preservatiort. Normal values ranged from l to 13 U/1. Between-day reproducibility was estimated with two different commercial control serä, and the coefficient of Variation was 5% for the upper limit of normal activity (23 U/l). There was good agreement between the present method and a semi-automätic colorimetric technique (for 100 sera tested by both methods, the correlation coefficient was 0.974 and the regression line equation, y = 0.85 x-1.5). Despite the lengthy reagent mixture preparation procedure, the method permitted assay of 50 samples per hour. The occurrence of high serum blanks in certain pathological states is discussed. Kontinuierliche Bestimmung der katalytischen Aktivität von 5'±Nucleotidase im Serum mit Inosin-5'-monophosphat als Substrat und völliger Mechanisierung mit einem Transfer-Analyser (Kem-O-Mat) Zusammenfassung

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Measurement of Xanthine Oxidase Activity in Some Human Tissues

Cited by 40 publications

References 7 publications

Hepatoprotective effect of total flavonoids from Laggera alata against carbon tetrachloride-induced injury in primary cultured neonatal rat hepatocytes and in rats with hepatic damage

Hepatoprotective effect of total flavonoids from Laggera alata against carbon tetrachloride-induced injury in primary cultured neonatal rat hepatocytes and in rats with hepatic damage

Xanthine Oxidase during Human Fetal Development

Continuous Assay of Serum 5'-Nucleotidase Activity with Inosine 5'-Monophosphate as Substrate and Total Automation Using a Transfer-Analyzer (Kem-O-Mat)

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