Hepatoprotective effect of total flavonoids from Laggera alata against carbon tetrachloride-induced injury in primary cultured neonatal rat hepatocytes and in rats with hepatic damage

Abstract

SummaryThe hepatoprotective activities of total flavonoids of Laggera alata (TFLA) were evaluated by carbon tetrachloride (CCl 4 )-induced injury in primary cultured neonatal rat hepatocytes and in rats with hepatic damage. In vitro, TFLA at a concentration range of 1-100 lg/ml improved cell viability and inhibited cellular leakage of two enzymes, hepatocyte aspartate aminotransferase (AST) and alanine aminotransferase (ALT), caused by CCl 4 . In vivo, oral treatment with TFLA at doses of 50, 100, and 200 mg/kg significantly reduced the levels of AST, ALT, total protein, and albumin in serum and the hydroxyproline and sialic acid levels in liver. Histopathological examinations revealed that liver damage were improved when treated with TFLA. Meanwhile, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide radicals scavenging activities of TFLA were also determinated. To understand the exact components of TFLA responsible for the hepatoprotective effect, nine flavonoid compounds were isolated and identified from TFLA. In conclusion, the present investigation was the first to verify the hepatoprotective effect of L. alata in vitro and in vivo. The hepatoprotective action of TFLA is likely related to its potent antioxidative and anti-inflammatory activity. Neutralizing reactive oxygen species by nonenzymatic mechanisms and enhancing the activity of original natural hepatic-antioxidant enzymes may be the main mechanisms of TFLA against CCl 4 -induced injury.

Hexane Extract of Dacryodes Edulis Fruits Possesses Anti-Diabetic and Hypolipidaemic Potentials in Alloxan Diabetes of Rats.

“…At the same dose, D. edulis positively regulated glycaemia, restored the desirable lipids while not adversely affecting centres of metabolism and excretion, the liver and kidneys, as observed by the liver function markers, creatinine and urea results. Despite the diabetic state being associated with hepatocellular damage and leakages to cause a rise in liver marker levels such as AST, ALT and ALP, and also plant phytochemicals especially flavonoids associated with reversing such (Tapas et al 2008; Wu et al 2006), our experimental animal's liver enzymes were largely unchanged by the diabetic state. However, D. edulis extract was observed to reduce the ALT and ALP levels on day 15, similar to glibenclamide.…”

Hepatoprotective Effects of Tribulus terrestris Hydro-Alcholic Extract on Non-Alcoholic Fatty Liver- Induced Rats

“…Flavonoids improved cell viability and inhibited cellular leakage of hepatocyte AST and ALT (Su et al). Further, it is been shown that flavonoids can decrease the insulin resistance and insulin requirements in adipose and muscle tissue (Wu et al, 2006). Besides flavonoids, the plant contains alkaloid, glycosides, steroids and saponin that have hypolipidemic properties (Divya et al, 2014).…”

Chemical composition and biological activities of the essential oil of Laggera aurita Linn (DC.) grown in Pakistan

“…Bactericidal properties of the volatile oil are reported by Geda in 1995 [16]. Other species of Laggera such as L. pterodonta has been employed in herbal medicine in China as anti inflammatory agent for treatment of hepatitis, arthritis, bronchitis and nephritis [17][18]. However, no significant work has been reported on chemical composition and bioactivity related studies of the species found in Pakistan.…”