Interlaboratory collaboration to determine the performance of the Randox food diagnostics biochip array technology for the simultaneous quantitative detection of seven mycotoxins in feed

Abstract

An inter-laboratory collaborative study was performed to evaluate the performance of the Biochip Array Technology (BAT) Myco 7 method. The Myco 7 Array is a method which simultaneously and quantitatively detects 20 mycotoxins including aflatoxins B1, B2, G1 and G2, ochratoxin A, deoxynivalenol, zearalenone, fumonisin B1, B2 and B3 and T-2 and HT-2 toxin. The BAT Myco 7 method was collaboratively evaluated by nine government and private Association of American Feed Control Officials (AAFCO) laboratories. Samples were analysed in a proficiency testing round format. Seventeen blind samples were analysed on the same equipment using Myco 7 kits. 99% of the results fell within an acceptable Z-score range of -2|<Z<|+2. Deoxynivalenol had a 100% Z-score pass rate, while a 99% pass was recorded for aflatoxins, zearalenone, ochratoxin A and fumonisins. T-2 toxin had a 97% Z-score pass rate. HorRat analysis for reproducibility used a range of 0.3<|HorRat|≤2. The target was met for deoxynivalenol, zearalenone, T-2 and HT-2 toxin, and aflatoxins B1, B2, G1 and G2 assays. Fumonisins and ochratoxin A assays had a 93% and 94% pass, respectively. The reproducibility co-efficiency of variation was between 16 and 20% meeting set criterion of <40% and is, therefore, fit-for-purpose for use in the AAFCO control programs for mycotoxins.

T-2 and HT-2 Toxins: Toxicity, Occurrence and Analysis: A Review

“…An alternative commercially available chemiluminescence immunoassay technique known as Biochip Array Technology manufactured by Randox Laboratories Ltd. was developed for the simultaneous detection of seven mycotoxins in animal feed. A single laboratory validation showed ccα and ccβ concentrations of 3.70 µg/kg and 4.84 µg/kg, respectively, for T-2 toxin [ 169 ]. Recently, in an inter-laboratory study, the test kit was found to have a 97% Z-score pass rate [ 170 ].…”

“…Recently, in an inter-laboratory study, the test kit was found to have a 97% Z-score pass rate [ 170 ]. However, the cross-reactivity of the antibody is 100% for T-2 toxin and only 37% against HT-2 toxin [ 169 ]; therefore, the kit is not applicable to the analysis of the sum of T-2 and HT-2 toxin.…”

Developments in mycotoxin analysis: an update for 2021-22

“…The study was designed across nine American Association of Feed Control Officials laboratories, by using 15 reference materials obtained from the laboratories' previous PT rounds. Meeting the set criteria (Z-score interval of -2<Z<+2 and 0.3 |HorRat|≤2) for almost 100% for all of the analytes, and reproducibility co-efficiency of variation as low as 16-20%) made this method fit-for-purpose for its use in the control programs for mycotoxins (Sibanda et al, 2022). Contrary to LC-MS methods, the biochip array technology is definitely less complicated for implementation, cheaper, and easier in terms of data evaluation.…”

Performance Evaluation of Biochip Chemiluminescent Immunoassay for Screening Seven Mycotoxins in Wheat Flour Simultaneously

“…Biochip array technology enhances result throughput and offers significant cost-effectiveness advantages for mycotoxin screening ( 17 ). The testing procedure for the Myco 7 kit is user-friendly, and the generated results are easily interpretable ( 18 ). This method is relatively fast and allows for the simultaneous testing of mycotoxins.…”