Intergeneric hybridization of Trichoderma reesei QM9414 and Saccharomyces cerevisiae NCIM 3288 by protoplast fusion

Kumari, Jyoti; Panda, T.

doi:10.1016/0141-0229(94)90062-0

Cited by 14 publications

(14 citation statements)

References 40 publications

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“…Regeneration is carried out using complete glucose mineral medium [16]. The regeneration frequency is de®ned as the ratio of the number of colonies regenerating from the protoplasts to the total number of plated protoplasts.…”

Section: Regeneration Of Protoplastsmentioning

confidence: 99%

“…The molecular weight of polyethylene glycol used for fusion varied from 4000 [2] to 6000 [17]. Mutant strains were obtained by exposure to UV-rays [16]. They were then screened for the stable strain.…”

Section: Chemofusion Techniquementioning

confidence: 99%

“…Special`k nobs'' were studied in Trichoderma reesei cells by Toyama et al [17]. Colour of the fusant strain was used to differentiate between wild fusants and mutant strains [16]. Fusants can also be characterized by their genotypic expressions.…”

Section: Chemofusion Techniquementioning

confidence: 99%

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Fungal protoplast fusion – a revisit

Muralidhar

Panda

2000

Bioprocess Engineering

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Section: Regeneration Of Protoplastsmentioning

confidence: 99%

Section: Chemofusion Techniquementioning

confidence: 99%

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Fungal protoplast fusion – a revisit

Muralidhar

Panda

2000

Bioprocess Engineering

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“…So far only multistage approach of ethanol production has been taken up, which has its own limitations of being capital intensive, time consuming and low yielding [4]. Hence, in order to achieve a single stage conversion of cellulose to ethanol, Trichoderma reesei QM 9414, a hyper-cellulase producer, was fused with Saccharomyces cerevisiae NCIM 3288, a potent ethanol producer, in the authors' laboratory [5].The resultant fusant has been found to be stable and produce ethanol from cellulosic substances [6]. Production of endoglucanase, one of the enzymes responsible for the conversion of cellulosic materials to fermentable sugars, is a key factor in this case.…”

mentioning

confidence: 99%

“…The intergeneric fusants of Trichoderma reesei QM 9414/Saccharomyces cerevisiae NCIM 3288 was developed in the authors' laboratory[5].T. reesei QM 9414 was maintained on malt-agar slants, having (kg/m 3 ): malt-extract, 30; agar-agar, 25.…”

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confidence: 99%

Comparative analysis of optimal endoglucanase production by Trichoderma reesei and intergeneric fusants of Trichoderma reesei Saccharomyces cerevisiae

Sinha

Panda

1998

Bioprocess Engineering

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Intergeneric fusants of Trichoderma reesei QM 9414/Saccharomyces cerevisiae NCIM 3288 developed in the authors' laboratory can convert cellulosic materials directly to ethanol in a single step process. The production of endoglucanase in this case is a key factor. The production pro®le of this enzyme by the intergeneric fusants is different from Trichoderma reesei QM 9414 (WT). The production of endoglucanase was studied seperately by Trichoderma reesei (WT) using optimal production medium which was designed as per the combined screening approach of Plackett-Burman followed by a central composite experimental plan and the intergeneric fusants using optimal production medium obtained by Box-Behnken optimization procedure. Dried grass was used as the cellulosic substance whose concentration was kept constant during the statistical optimization procedure. The concentration of dried grass was later varied keeping the other optimized medium constituents constant to ®nd the ®nal optimum medium composition for endoglucanase production. List of symbolsvalue of x i at the centre point of the investigated area Dx i step change IntroductionWith the reserves of non-replenishable energy sources slowly getting exhausted, efforts are on for tapping energy from the replenishable ones. Since ethanol can be obtained from renewable resources, it provides a promising alternative fuel source. Besides, ethanol is also used as a solvent, a germicide, an antifreeze and a chemical intermediate [1]. Ethanol is produced by fermentation by the S. cerevisiae-molasses technology. However, scanty and uneven supply of molasses and its higher consumption for more costly chemical and biochemical production renders severe handicap to this technology [2,3]. Ethanol is also produced from cellulose, an abundant biomass. So far only multistage approach of ethanol production has been taken up, which has its own limitations of being capital intensive, time consuming and low yielding [4]. Hence, in order to achieve a single stage conversion of cellulose to ethanol, Trichoderma reesei QM 9414, a hyper-cellulase producer, was fused with Saccharomyces cerevisiae NCIM 3288, a potent ethanol producer, in the authors' laboratory [5].The resultant fusant has been found to be stable and produce ethanol from cellulosic substances [6]. Production of endoglucanase, one of the enzymes responsible for the conversion of cellulosic materials to fermentable sugars, is a key factor in this case. Comparative analysis of endoglucanase production by the intergeneric fusants of T. reesei/S. cerevisiae and T. reesei (WT) under optimal production conditions using response-surface methodology has been presented. 2 Materials and methods 2.1 Microorganisms Trichoderma reesei QM 9414 was obtained from the National Chemical Laboratory, Pune, India. The intergeneric fusants of Trichoderma reesei QM 9414/Saccharomyces cerevisiae NCIM 3288 was developed in the authors' laboratory [5]. 2.2 Culture maintenance T. reesei QM 9414 was maintained on malt-agar slants, having (kg/m 3 ): malt-e...

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Penicillium citrinum protoplasts: Preparation, regeneration and lipolytic activity of regenerants

Maliszewska

Zboińska

1996

Acta Biotechnologica

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The conditions for an optimum formation of protoplasts from mycelium of Pencillium citrinum, which produces extracellular lipase, were examined. The best results were achieved using Novozyme 234 in combination with pglucuronidase. Chitinase or pglucuronidase alone were not able to produce protoplasts under the given conditions. For the regeneration frequency. the type of osmotic stabilizer was important. The highest regeneration frequency (84.6% protoplasts) was achieved by using 0.6 M KCl as an osmotic stabilizer. Some regenerants were more effective in producing lipase than the parent. In particular, strain no. P-8 showed a three times higher lipolytic activity than the parent strain, but this high enzyme activity was unstable after subculture.

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Intergeneric hybridization of Trichoderma reesei QM9414 and Saccharomyces cerevisiae NCIM 3288 by protoplast fusion

Cited by 14 publications

References 40 publications

Fungal protoplast fusion – a revisit

Fungal protoplast fusion – a revisit

Comparative analysis of optimal endoglucanase production by Trichoderma reesei and intergeneric fusants of Trichoderma reesei Saccharomyces cerevisiae

Penicillium citrinum protoplasts: Preparation, regeneration and lipolytic activity of regenerants

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