Fungal protoplast fusion – a revisit

Muralidhar, R. V.; Panda, T.

doi:10.1007/s004490050755

Cited by 28 publications

(16 citation statements)

References 13 publications

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“…On fusion of parent protoplasts, the progeny may express characteristics of either parent or a hybrid expression. 74,75 Genome shuffling was applied for breeding the native paclitaxel-producing fungus, Nodulisporum sylviforme strain HQD 33 . 76 First, N. sylviforme spores were treated in several mutagenesis rounds (UV, EMS 60 Co and NTG) to obtain the strain NCEU-1.…”

Section: Genome Shufflingmentioning

confidence: 99%

Microbial paclitaxel: advances and perspectives

et al. 2010

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Paclitaxel is a potent and widely used antitumor agent. Considerable worldwide research efforts have been carried out on different production alternatives. Since the description of the first paclitaxel-producing fungi, more than 15 years ago, microorganisms have been investigated as potential alternatives for an environmentally acceptable, relatively simple and inexpensive method to produce paclitaxel. However, in spite of significant research on paclitaxel-producing microorganisms, no commercial fermentation process has been implemented up to now. The aim of this study is to review the present status of research on paclitaxel-producing microorganisms and the ongoing efforts to develop heterologous paclitaxel biosynthesis, and analyze the perspectives of microbial fermentation for paclitaxel production.

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Section: Genome Shufflingmentioning

confidence: 99%

Microbial paclitaxel: advances and perspectives

et al. 2010

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“…Protoplast fusion technique has a great potential for genetic analysis and for strain improvement. It is particularly useful for industrially useful micro‐organisms (Murlidhar and Panda ). These techniques have been widely used for enhanced yield in conversion of cellulose to ethanol (Knowles et al .…”

Section: Introductionmentioning

confidence: 99%

“…Protoplast fusion technique has a great potential for genetic analysis and for strain improvement. It is particularly useful for industrially useful micro-organisms (Murlidhar and Panda 2000). These techniques have been widely used for enhanced yield in conversion of cellulose to ethanol (Knowles et al 1987), strain improvement for alcohol fermentation (Lima et al 1995), citric acid-producing strains of Aspergillus niger (Kirimura et al 1986).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of intergeneric hybrid betweenAspergillus oryzaeandTrichoderma harzianumby protoplast fusion

Patil

Govindwar

et al. 2015

J Appl Microbiol

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“…It breaks down the barriers to genetic exchange and is a relatively new flexible technique to induce or promote genetic recombination in a variety of prokaryotic and eukaryotic cells [11]. By protoplast fusion (Figure 1), interspecific or even intergeneric hybrids can be produced, and it is feasible to transfer useful genes, for attributes (such as disease resistance, enzyme and phytotoxin production, rapid growth rate, nitrogen fixation, protein quality, and drought resistance), from one species to another [12].…”

Section: Use Of Protoplast Fusionmentioning

confidence: 99%

Application of Radiation and Genetic Engineering Techniques to Improve Biocontrol Agent Performance: A Short Review

Mirmajlessi¹,

Mostafavi²,

Loit³

et al. 2019

Use of Gamma Radiation Techniques in Peaceful Applications

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Biological control is a potential nonchemical method to manage plant pathogens by beneficial microorganisms. To improve antagonistic potential of biocontrol agents, mutation by radiations, chemicals, and genetic manipulations has been used. Genetic techniques and ionizing radiation containing direct or indirect emissions play the greatest role for selection of useful microorganisms to enhance the efficiency of biological systems. Indeed, genetic engineering has a main role in increasing antimicrobial metabolites, host colonization ability, and endurance in micro-ecosystem. Genetic improvement can be achieved by protoplast fusion, genetic modification (GM), and chemical (genotoxic agents) and physical mutations. However, ultraviolet light and ionizing radiations can induce modifications in the genome of an organism. Irradiation, particularly gamma rays, is also applied for controlling postharvest diseases. Indeed, irradiation cannot completely eliminate pathogens, but it might result in cell injury and directly damage the chromosomal DNA of a living cell. This technology has been used for many reasons including disinfestation of foods, reducing foodborne pathogens, and extending shelf life many fruits, vegetables, and nuts. In the current review, we discuss advances in the radiation and molecular genetic techniques with the aim to improve antagonistic potential of microorganisms as it is applied to the suppression of plant pathogens.

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Fungal protoplast fusion – a revisit

Cited by 28 publications

References 13 publications

Microbial paclitaxel: advances and perspectives

Microbial paclitaxel: advances and perspectives

Molecular characterization of intergeneric hybrid betweenAspergillus oryzaeandTrichoderma harzianumby protoplast fusion

Application of Radiation and Genetic Engineering Techniques to Improve Biocontrol Agent Performance: A Short Review

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